Construct: sgRNA BRDN0001149439
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGGCTTCGAGACAGCCAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TESK2 (10420)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76435
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
45345469 |
+ |
TESK2 |
NNNGCTTCGAGACAGCCAGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
70673418 |
- |
SHANK2 |
NNNGCTTTGAGACAGACAGA |
NGG |
2 |
0.875 |
Tier II |
| 3 |
NC_000019.10 |
19 |
48297846 |
- |
ODAD1 |
NNNGCTTCGAGGCAGCCAGA |
NGG |
1 |
0.7222 |
Tier II |
| 4 |
NC_000001.11 |
1 |
156162932 |
- |
SEMA4A |
NNNGCCTGGAGACAGCCAGA |
NGG |
2 |
0.5844 |
Tier II |
| 5 |
NC_000002.12 |
2 |
101036629 |
+ |
TBC1D8 |
NNNGCTTCCTGACAGCCAGA |
NGG |
2 |
0.4751 |
Tier II |
| 6 |
NC_000023.11 |
X |
107634577 |
+ |
PRPS1 |
NNNGCTTCCTGACAGCCAGA |
NGG |
2 |
0.4751 |
Tier II |
| 7 |
NC_000007.14 |
7 |
6701768 |
- |
ZNF12 |
NNNGCCTCGAGTCAGCCAGA |
NGG |
2 |
0.303 |
Tier II |
| 8 |
NC_000022.11 |
22 |
44160467 |
- |
PARVB |
NNNGCTTCGAGCCAGCCACA |
NGG |
2 |
0.118 |
Tier II |
| 9 |
NC_000001.11 |
1 |
19717673 |
- |
TMCO4 |
NNNGCTTCGAGGCAGGCAGA |
NGG |
2 |
0.1111 |
Tier II |
| 10 |
NC_000003.12 |
3 |
10023764 |
+ |
CIDECP1 |
NNNGCATCGAGACAACCAGA |
NGG |
2 |
0.8157 |
Tier III |
| 11 |
NC_000008.11 |
8 |
34199962 |
- |
LOC105379364 |
NNNGCTGCAAGACAGCCAGA |
NGG |
2 |
0.3782 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
116802687 |
- |
Tesk2 |
NNNGCTTCGAGACAGCCAGA |
NAG |
1 |
0.2593 |
Tier I |
| 2 |
NC_000071.6 |
5 |
51792778 |
- |
Ppargc1a |
NNNGCTTTGAGACAGACAGA |
NGG |
2 |
0.875 |
Tier II |
| 3 |
NC_000084.6 |
18 |
42861846 |
+ |
Ppp2r2b |
NNNGCTTTGAGACAGCCAGA |
NGG |
1 |
0.875 |
Tier II |
| 4 |
NC_000081.6 |
15 |
64921433 |
- |
Adcy8 |
NNNGCTTAGAGACAGCCAGG |
NGG |
2 |
0.4971 |
Tier II |
| 5 |
NC_000077.6 |
11 |
88499164 |
- |
Msi2 |
NNNGCTTCGAGACTGCCAGG |
NGG |
2 |
0.4078 |
Tier II |
| 6 |
NC_000075.6 |
9 |
121690664 |
- |
Sec22c |
NNNGCTTAGAGACAGCCTGA |
NGG |
2 |
0.325 |
Tier II |
| 7 |
NC_000068.7 |
2 |
102177257 |
+ |
Ldlrad3 |
NNNGATTCCAGACAGCCAGA |
NGG |
2 |
0.3077 |
Tier II |
| 8 |
NC_000073.6 |
7 |
125765616 |
+ |
D430042O09Rik |
NNNGCTTCCAGACAGCTAGA |
NGG |
2 |
0.2513 |
Tier II |
| 9 |
NC_000069.6 |
3 |
37410548 |
- |
Nudt6 |
NNNGCTTCCAGAAAGCCAGA |
NGG |
2 |
0.2071 |
Tier II |
| 10 |
NC_000072.6 |
6 |
38853455 |
+ |
Hipk2 |
NNNGCTTAGAGACAGGCAGA |
NGG |
2 |
0.1 |
Tier II |
| 11 |
NC_000067.6 |
1 |
39303950 |
+ |
Npas2 |
NNNGCTTCAAGACAGCCAGA |
NGT |
2 |
0.0104 |
Tier II |
| 12 |
NC_000071.6 |
5 |
119666394 |
+ |
Tbx3os1 |
NNNGCTTAGAAACAGCCAGA |
NGG |
2 |
0.65 |
Tier III |
| 13 |
NC_000078.6 |
12 |
32735969 |
+ |
Gm40376 |
NNNGCTTCCAGACAGCTAGA |
NGG |
2 |
0.2513 |
Tier III |
| 14 |
NC_000085.6 |
19 |
25853232 |
- |
Gm34516 |
NNNGCTTCTAGACAGCTAGA |
NGG |
2 |
0.2489 |
Tier III |
Other clones with same target sequence:
(none)
