Construct: sgRNA BRDN0001149444
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAAGTCCATATCCTTAAGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP4K2C (79837)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77682
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
57600343 |
- |
PIP4K2C |
NNNGTCCATATCCTTAAGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
24725878 |
- |
GSDME |
NNNGTCCACTTCCTTAAGGG |
NGG |
2 |
0.8145 |
Tier II |
| 3 |
NC_000024.10 |
Y |
20757183 |
- |
RPS4Y2 |
NNNGGACATATCCTTAAGGG |
NGG |
2 |
0.5943 |
Tier II |
| 4 |
NC_000010.11 |
10 |
99618153 |
- |
SLC25A28 |
NNNGTGCATGTCCTTAAGGG |
NGG |
2 |
0.2778 |
Tier II |
| 5 |
NC_000016.10 |
16 |
53970237 |
- |
FTO |
NNNGTCCATATGATTAAGGG |
NGG |
2 |
0.1709 |
Tier II |
| 6 |
NC_000009.12 |
9 |
134155961 |
+ |
WDR5 |
NNNGTCCATCTCCTTAACGG |
NGG |
2 |
0.1587 |
Tier II |
| 7 |
NC_000005.10 |
5 |
53928654 |
- |
ARL15 |
NNNGTCCATATACTTGAGGG |
NGG |
2 |
0.1374 |
Tier II |
| 8 |
NC_000009.12 |
9 |
124597458 |
- |
NR6A1 |
NNNATCCATATCCTTAAGGG |
NGA |
2 |
0.0625 |
Tier II |
| 9 |
NC_000001.11 |
1 |
28536177 |
+ |
RCC1 |
NNNGTCCATATCCTGATGGG |
NGG |
2 |
0.0067 |
Tier II |
| 10 |
NC_000003.12 |
3 |
8853952 |
- |
LOC107984112 |
NNNGTCCACATCCCTAAGGG |
NGG |
2 |
0.2637 |
Tier III |
| 11 |
NC_000003.12 |
3 |
16129394 |
+ |
LOC124906217 |
NNNGTCCATATTCTTACGGG |
NGG |
2 |
0.095 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
74149083 |
- |
Pcdh15 |
NNNGTACATATCCTTAAGTG |
NGG |
2 |
0.619 |
Tier II |
| 2 |
NC_000078.6 |
12 |
109587908 |
- |
Rtl1 |
NNNGTCCATAACCTTAAGGT |
NGG |
2 |
0.525 |
Tier II |
| 3 |
NC_000071.6 |
5 |
20079845 |
+ |
Magi2 |
NNNGTCCATATCCCTAAAGG |
NGG |
2 |
0.1978 |
Tier II |
| 4 |
NC_000078.6 |
12 |
83482555 |
- |
Dpf3 |
NNNGTCCATATCCATAAGGG |
NAG |
2 |
0.1605 |
Tier II |
| 5 |
NC_000071.6 |
5 |
52543496 |
+ |
Lgi2 |
NNNGTCCCTATCCCTAAGGG |
NGG |
2 |
0.1224 |
Tier II |
| 6 |
NC_000082.6 |
16 |
16783455 |
- |
Spag6l |
NNNGTCCATATCATTAAGGG |
NAG |
2 |
0.0997 |
Tier II |
| 7 |
NC_000082.6 |
16 |
22071136 |
+ |
Igf2bp2 |
NNNGTCCATATTCTTATGGG |
NGG |
2 |
0.0718 |
Tier II |
| 8 |
NC_000072.6 |
6 |
32842090 |
+ |
Chchd3 |
NNNGTCCATTTCCTTAAGGG |
NTG |
2 |
0.0344 |
Tier II |
| 9 |
NC_000078.6 |
12 |
56970285 |
+ |
Slc25a21 |
NNNTTCCATATCCTTAAGGG |
NTG |
2 |
0.0142 |
Tier II |
| 10 |
NC_000073.6 |
7 |
25293577 |
- |
Cic |
NNNGTCCATATCCTGAGGGG |
NGG |
2 |
0.0088 |
Tier II |
| 11 |
NC_000067.6 |
1 |
93540692 |
+ |
Farp2 |
NNNGTCCATGTCCTTTAGGG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000077.6 |
11 |
69256803 |
- |
Gm40039 |
NNNGTCCATACCATTAAGGG |
NGG |
2 |
0.2564 |
Tier III |
| 13 |
NC_000067.6 |
1 |
93540692 |
+ |
Gm41920 |
NNNGTCCATGTCCTTTAGGG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
