Construct: sgRNA BRDN0001149450
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATAGATGCGATCCACCAGTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK38L (23012)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76630
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
27314604 |
+ |
STK38L |
NNNGATGCGATCCACCAGTT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
27129335 |
- |
HOXA4 |
NNNGATGCGATCCTCCAATT |
NGG |
2 |
0.3692 |
Tier I |
| 3 |
NC_000004.12 |
4 |
4550019 |
+ |
LOC124900165 |
NNNGAAGCGATCTACCAGTT |
NGG |
2 |
0.6067 |
Tier II |
| 4 |
NC_000007.14 |
7 |
27129335 |
- |
HOXA3 |
NNNGATGCGATCCTCCAATT |
NGG |
2 |
0.3692 |
Tier II |
| 5 |
NC_000003.12 |
3 |
156306418 |
+ |
KCNAB1 |
NNNGATGGGATCCTCCAGTT |
NGG |
2 |
0.3429 |
Tier II |
| 6 |
NC_000002.12 |
2 |
69754583 |
- |
ANXA4 |
NNNGATGGGATCCAGCAGTT |
NGG |
2 |
0.0321 |
Tier II |
| 7 |
NC_000004.12 |
4 |
4550019 |
+ |
STX18-AS1 |
NNNGAAGCGATCTACCAGTT |
NGG |
2 |
0.6067 |
Tier III |
| 8 |
NC_000018.10 |
18 |
42674965 |
- |
LINC00907 |
NNNGATTCGATCCACCAGTT |
NGT |
2 |
0.0092 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
76747855 |
- |
Ttn |
NNNCATGCGATCCACCAGGT |
NGG |
2 |
0.1324 |
Tier I |
| 2 |
NC_000076.6 |
10 |
80022240 |
+ |
Arhgap45 |
NNNGATGCCACCCACCAGTT |
NGG |
2 |
0.359 |
Tier II |
Other clones with same target sequence:
(none)
