Construct: sgRNA BRDN0001149453
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCTGCACAGCTATAAGAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK17 (5128)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76786
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
96298874 |
- |
CDK17 |
NNNTGCACAGCTATAAGAGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
14371192 |
- |
NFIB |
NNNTGCATAGCTATAAGAAA |
NGG |
2 |
0.625 |
Tier II |
| 3 |
NC_000011.10 |
11 |
79159141 |
+ |
TENM4 |
NNNTGCAGAGCTATAAGAGG |
NGG |
2 |
0.4916 |
Tier II |
| 4 |
NC_000008.11 |
8 |
86122677 |
+ |
ATP6V0D2 |
NNNGCCACAGCTATAAGAGA |
NGG |
2 |
0.4911 |
Tier II |
| 5 |
NC_000012.12 |
12 |
18629265 |
+ |
PIK3C2G |
NNNTGCACAGATATGAGAGA |
NGG |
2 |
0.4875 |
Tier II |
| 6 |
NC_000001.11 |
1 |
58418160 |
- |
DAB1 |
NNNAGCAAAGCTATAAGAGA |
NGG |
2 |
0.3095 |
Tier II |
| 7 |
NC_000006.12 |
6 |
56362795 |
- |
COL21A1 |
NNNTGCACAGCAACAAGAGA |
NGG |
2 |
0.2286 |
Tier II |
| 8 |
NC_000011.10 |
11 |
24740231 |
+ |
LUZP2 |
NNNTGCACAGCTATAAGCTA |
NGG |
2 |
0.127 |
Tier II |
| 9 |
NC_000008.11 |
8 |
56960233 |
+ |
BPNT2 |
NNNTGCTCAGCTATCAGAGA |
NGG |
2 |
0.1193 |
Tier II |
| 10 |
NC_000004.12 |
4 |
8078971 |
+ |
ABLIM2 |
NNNTGCACAGCTACTAGAGA |
NGG |
2 |
0.0571 |
Tier II |
| 11 |
NC_000011.10 |
11 |
106693047 |
+ |
GUCY1A2 |
NNNTGCAGAGCTATAAGAGA |
NTG |
2 |
0.025 |
Tier II |
| 12 |
NC_000011.10 |
11 |
111909778 |
+ |
CRYAB |
NNNTGCACAGCTAAAAGAGA |
NGT |
2 |
0.01 |
Tier II |
| 13 |
NC_000005.10 |
5 |
155785445 |
- |
SGCD |
NNNTGCACAGCTAGAAGAGA |
NGA |
2 |
0.0 |
Tier II |
| 14 |
NC_000008.11 |
8 |
7786840 |
+ |
LOC124901865 |
NNNTGCACAGCTATAAAAAA |
NGG |
2 |
0.6667 |
Tier III |
| 15 |
NC_000012.12 |
12 |
91583066 |
- |
LOC105369896 |
NNNTACACAGCTCTAAGAGA |
NGG |
2 |
0.1825 |
Tier III |
| 16 |
NC_000008.11 |
8 |
103246058 |
+ |
LOC105369147 |
NNNTGCAGAGCTATCAGAGA |
NGG |
2 |
0.1753 |
Tier III |
| 17 |
NC_000010.11 |
10 |
112892956 |
- |
LINC02935 |
NNNTGCACAGCTATCAGAGT |
NGG |
2 |
0.1636 |
Tier III |
| 18 |
NC_000005.10 |
5 |
2064349 |
- |
LOC105374618 |
NNNAGCACAGCTATACGAGA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
93225046 |
+ |
Cdk17 |
NNNTGTACAGCTATAAGAGA |
NGG |
1 |
0.9286 |
Tier I |
| 2 |
NC_000071.6 |
5 |
121817802 |
+ |
Sh2b3 |
NNNTGCACAGCTGTGAGAGA |
NGG |
2 |
0.4239 |
Tier I |
| 3 |
NC_000075.6 |
9 |
64133105 |
+ |
Lctl |
NNNTTCACAGCTATAAAAGA |
NGG |
2 |
0.28 |
Tier I |
| 4 |
NC_000080.6 |
14 |
61212638 |
+ |
Sacs |
NNNTGCAAAGCTCTAAGAGA |
NGG |
2 |
0.1368 |
Tier I |
| 5 |
NC_000076.6 |
10 |
24775257 |
- |
Enpp3 |
NNNTACACAGCCATAAGAGA |
NGG |
2 |
0.8211 |
Tier II |
| 6 |
NC_000067.6 |
1 |
155377164 |
- |
Xpr1 |
NNNTACACAGCTATAAAAGA |
NGG |
2 |
0.8089 |
Tier II |
| 7 |
NC_000086.7 |
X |
138994606 |
+ |
Nrk |
NNNTGCAGAGATATAAGAGA |
NGG |
2 |
0.4821 |
Tier II |
| 8 |
NC_000076.6 |
10 |
3754367 |
- |
Plekhg1 |
NNNTGCTCAGCTATAAGAGA |
NGG |
1 |
0.4375 |
Tier II |
| 9 |
NC_000069.6 |
3 |
79828063 |
+ |
Tmem144 |
NNNTGGACAGCTGTAAGAGA |
NGG |
2 |
0.3261 |
Tier II |
| 10 |
NC_000083.6 |
17 |
25310682 |
- |
Prss28 |
NNNTGCAGAGCTATAAGTGA |
NGG |
2 |
0.3214 |
Tier II |
| 11 |
NC_000081.6 |
15 |
81628964 |
- |
Ep300 |
NNNTGCTCAGCTATAAGACA |
NGG |
2 |
0.1961 |
Tier II |
| 12 |
NC_000072.6 |
6 |
40141469 |
- |
Tmem178b |
NNNTGAACAGCTCTAAGAGA |
NGG |
2 |
0.1955 |
Tier II |
| 13 |
NC_000070.6 |
4 |
141052397 |
- |
Crocc |
NNNGGCACAGCTTTAAGAGA |
NGG |
2 |
0.1875 |
Tier II |
| 14 |
NC_000080.6 |
14 |
70261349 |
- |
Ppp3cc |
NNNTGCTCACCTATAAGAGA |
NGG |
2 |
0.175 |
Tier II |
| 15 |
NC_000068.7 |
2 |
153517705 |
- |
Nol4l |
NNNTGCACAGCTGTAAGAGA |
NAG |
2 |
0.1691 |
Tier II |
| 16 |
NC_000077.6 |
11 |
82939395 |
+ |
Unc45b |
NNNGGCACAGGTATAAGAGA |
NGG |
2 |
0.1563 |
Tier II |
| 17 |
NC_000073.6 |
7 |
37676408 |
- |
Zfp536 |
NNNTGCACAGCTATAGGAGG |
NGG |
2 |
0.1471 |
Tier II |
| 18 |
NC_000069.6 |
3 |
138443976 |
+ |
Adh5 |
NNNAGCACAGTTATAAGAGA |
NGG |
2 |
0.1465 |
Tier II |
| 19 |
NC_000071.6 |
5 |
136088391 |
+ |
Rasa4 |
NNNTGCTCAGCTACAAGAGA |
NGG |
2 |
0.125 |
Tier II |
| 20 |
NC_000086.7 |
X |
77555243 |
- |
Tbl1x |
NNNTTCACAGTTATAAGAGA |
NGG |
2 |
0.0923 |
Tier II |
| 21 |
NC_000072.6 |
6 |
56740109 |
- |
Avl9 |
NNNTTCACAGCTATCAGAGA |
NGG |
2 |
0.0818 |
Tier II |
| 22 |
NC_000071.6 |
5 |
86716776 |
+ |
Tmprss11e |
NNNTGCTCAGCTATAAGAGA |
NTG |
2 |
0.017 |
Tier II |
| 23 |
NC_000067.6 |
1 |
52788516 |
+ |
Inpp1 |
NNNTGCACAGCTCTAAGAGA |
NGT |
2 |
0.0034 |
Tier II |
| 24 |
NC_000075.6 |
9 |
28605194 |
- |
Opcml |
NNNTGCACAGCTAGAAGAGA |
NGT |
2 |
0.0 |
Tier II |
| 25 |
NC_000082.6 |
16 |
30523705 |
- |
Tmem44 |
NNNTGCACAGCTAGAAGAGG |
NGG |
2 |
0.0 |
Tier II |
| 26 |
NC_000083.6 |
17 |
36510631 |
- |
Gm18809 |
NNNTCCACATCTATAAGAGA |
NGG |
2 |
0.6384 |
Tier III |
| 27 |
NC_000076.6 |
10 |
3754367 |
- |
Gm30164 |
NNNTGCTCAGCTATAAGAGA |
NGG |
1 |
0.4375 |
Tier III |
| 28 |
NC_000081.6 |
15 |
10766072 |
+ |
4930556M19Rik |
NNNTGCACAGCTATAACAGA |
NTG |
2 |
0.0092 |
Tier III |
| 29 |
NC_000082.6 |
16 |
30523705 |
- |
Gm52252 |
NNNTGCACAGCTAGAAGAGG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
