Construct: sgRNA BRDN0001149457
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GACCTGGAACGGTACCACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SRC (6714)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75652
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
37400133 |
+ |
SRC |
NNNCTGGAACGGTACCACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
49744258 |
- |
TSKS |
NNNCTGGATCGGTCCCACCA |
NGG |
2 |
0.1286 |
Tier I |
| 3 |
NC_000001.11 |
1 |
112515050 |
+ |
WNT2B |
NNNCTGGAACTGTACCACCC |
NGG |
2 |
0.0874 |
Tier I |
| 4 |
NC_000002.12 |
2 |
231807781 |
- |
COPS7B |
NNNCTGGAACAGTACCAACA |
NGG |
2 |
0.5385 |
Tier II |
| 5 |
NC_000001.11 |
1 |
17624518 |
- |
ARHGEF10L |
NNNCAGGCACGGTACCACCA |
NGG |
2 |
0.2143 |
Tier II |
| 6 |
NC_000007.14 |
7 |
128242280 |
- |
LEP |
NNNCTGGAACGGTACCACCA |
NGT |
1 |
0.0161 |
Tier II |
| 7 |
NC_000001.11 |
1 |
2651841 |
+ |
TTC34 |
NNNCTGGAACGGTACCACCA |
NTT |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
157467449 |
+ |
Src |
NNNCTGGAACGGCACCACGA |
NGG |
2 |
0.0987 |
Tier I |
| 2 |
NC_000075.6 |
9 |
119193704 |
- |
Slc22a13 |
NNNCTGGAACAGTACCACCA |
NGC |
2 |
0.0222 |
Tier I |
| 3 |
NC_000078.6 |
12 |
17348822 |
- |
Nol10 |
NNNCAGGAACGGCACCACCA |
NGG |
2 |
0.3947 |
Tier II |
| 4 |
NC_000074.6 |
8 |
118849669 |
+ |
Cdh13 |
NNNCTGGACCCGTACCACCA |
NGG |
2 |
0.2449 |
Tier II |
| 5 |
NC_000076.6 |
10 |
93536979 |
+ |
Amdhd1 |
NNNCTGGAACAGTATCACCA |
NGG |
2 |
0.0667 |
Tier II |
| 6 |
NC_000077.6 |
11 |
103902039 |
- |
Nsf |
NNNCTGGTACGGTACCAGCA |
NGG |
2 |
0.0571 |
Tier II |
| 7 |
NC_000072.6 |
6 |
128135858 |
+ |
Tspan9 |
NNNCTGGAACGGCAGCACCA |
NGG |
2 |
0.0395 |
Tier II |
| 8 |
NC_000072.6 |
6 |
41145716 |
+ |
Tcrb |
NNNCTGGAACGGTTCCCCCA |
NGG |
2 |
0.0941 |
Tier III |
| 9 |
NC_000075.6 |
9 |
119221253 |
- |
Slc22a13b |
NNNCTGGAACAGTACCACCA |
NGC |
2 |
0.0222 |
Tier III |
Other clones with same target sequence:
(none)
