Construct: sgRNA BRDN0001149457

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GACCTGGAACGGTACCACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRC (6714)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75652

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000020.11 20 37400133 + SRC NNNCTGGAACGGTACCACCA NGG 0 1.0 Tier I
2 NC_000019.10 19 49744258 - TSKS NNNCTGGATCGGTCCCACCA NGG 2 0.1286 Tier I
3 NC_000001.11 1 112515050 + WNT2B NNNCTGGAACTGTACCACCC NGG 2 0.0874 Tier I
4 NC_000002.12 2 231807781 - COPS7B NNNCTGGAACAGTACCAACA NGG 2 0.5385 Tier II
5 NC_000001.11 1 17624518 - ARHGEF10L NNNCAGGCACGGTACCACCA NGG 2 0.2143 Tier II
6 NC_000007.14 7 128242280 - LEP NNNCTGGAACGGTACCACCA NGT 1 0.0161 Tier II
7 NC_000001.11 1 2651841 + TTC34 NNNCTGGAACGGTACCACCA NTT 2 0.0 Tier II
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 157467449 + Src NNNCTGGAACGGCACCACGA NGG 2 0.0987 Tier I
2 NC_000075.6 9 119193704 - Slc22a13 NNNCTGGAACAGTACCACCA NGC 2 0.0222 Tier I
3 NC_000078.6 12 17348822 - Nol10 NNNCAGGAACGGCACCACCA NGG 2 0.3947 Tier II
4 NC_000074.6 8 118849669 + Cdh13 NNNCTGGACCCGTACCACCA NGG 2 0.2449 Tier II
5 NC_000076.6 10 93536979 + Amdhd1 NNNCTGGAACAGTATCACCA NGG 2 0.0667 Tier II
6 NC_000077.6 11 103902039 - Nsf NNNCTGGTACGGTACCAGCA NGG 2 0.0571 Tier II
7 NC_000072.6 6 128135858 + Tspan9 NNNCTGGAACGGCAGCACCA NGG 2 0.0395 Tier II
8 NC_000072.6 6 41145716 + Tcrb NNNCTGGAACGGTTCCCCCA NGG 2 0.0941 Tier III
9 NC_000075.6 9 119221253 - Slc22a13b NNNCTGGAACAGTACCACCA NGC 2 0.0222 Tier III
Download CSV

Other clones with same target sequence:

(none)