Construct: sgRNA BRDN0001149461
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCATGCGGCTGCCATAAAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RIPK1 (8737)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76532
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
3105633 |
- |
RIPK1 |
NNNTGCGGCTGCCATAAAGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
165678736 |
+ |
CSRNP3 |
NNNTGCGGCTGCACTAAAGA |
NGG |
2 |
0.0824 |
Tier I |
| 3 |
NC_000022.11 |
22 |
44938667 |
- |
PHF21B |
NNNTGCAGCTGCCATAATGA |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000014.9 |
14 |
94589931 |
+ |
SERPINA5 |
NNNTGGGGCTGCCATAAAGA |
NGG |
1 |
0.5 |
Tier II |
| 5 |
NC_000012.12 |
12 |
112814389 |
- |
RPH3A |
NNNTGGGGCTGTCATAAAGA |
NGG |
2 |
0.2692 |
Tier II |
| 6 |
NC_000007.14 |
7 |
26345213 |
- |
SNX10 |
NNNTGTGGCTGCCATAAAGA |
NAG |
2 |
0.2407 |
Tier II |
| 7 |
NC_000008.11 |
8 |
27288679 |
- |
TRIM35 |
NNNTGTGGCTGCCATAACGA |
NGG |
2 |
0.1769 |
Tier II |
| 8 |
NC_000011.10 |
11 |
58579923 |
- |
ZFP91 |
NNNTGCGGATGCCAGAAAGA |
NGG |
2 |
0.0429 |
Tier II |
| 9 |
NC_000006.12 |
6 |
3105633 |
- |
LOC107986556 |
NNNTGCGGCTGCCATAAAGA |
NGG |
0 |
1.0 |
Tier III |
| 10 |
NC_000007.14 |
7 |
142359874 |
+ |
TRB |
NNNTGAGGCTGCCATAAAGG |
NGG |
2 |
0.7101 |
Tier III |
| 11 |
NC_000011.10 |
11 |
125690928 |
+ |
LOC105369551 |
NNNTGCGGCAGCCATAGAGA |
NGG |
2 |
0.1513 |
Tier III |
| 12 |
NC_000011.10 |
11 |
58579923 |
- |
ZFP91-CNTF |
NNNTGCGGATGCCAGAAAGA |
NGG |
2 |
0.0429 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
64887399 |
- |
Dennd4a |
NNNTGTGTCTGCCATAAAGA |
NGG |
2 |
0.5804 |
Tier II |
| 2 |
NC_000076.6 |
10 |
10688737 |
- |
Grm1 |
NNNTGCGGCTGCCTTAAAGA |
NGG |
1 |
0.5333 |
Tier II |
| 3 |
NC_000070.6 |
4 |
152059555 |
- |
Nol9 |
NNNTGAGGCTGCCACAAAGA |
NGG |
2 |
0.2532 |
Tier II |
| 4 |
NC_000071.6 |
5 |
88586307 |
+ |
Rufy3 |
NNNTGAGGCTGCCATAAAGA |
NAG |
2 |
0.2407 |
Tier II |
| 5 |
NC_000077.6 |
11 |
84527495 |
+ |
Lhx1os |
NNNAGCGGCTGCCAAAAAGA |
NGG |
2 |
0.2757 |
Tier III |
| 6 |
NC_000067.6 |
1 |
43306769 |
+ |
LOC115487086 |
NNNTGTGGCTGCCATAAAGA |
NTG |
2 |
0.0362 |
Tier III |
Other clones with same target sequence:
(none)
