Construct: sgRNA BRDN0001149465
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGTAGCTGGACCCTCCAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MYLK (4638)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77020
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
123732905 |
- |
MYLK |
NNNTAGCTGGACCCTCCAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
53261253 |
- |
TSHZ2 |
NNNTAGCTGGAGCCTCCAAA |
NGG |
2 |
0.4167 |
Tier II |
| 3 |
NC_000012.12 |
12 |
119850905 |
+ |
CIT |
NNNTATCTGGACCCACCAAG |
NGG |
2 |
0.386 |
Tier II |
| 4 |
NC_000020.11 |
20 |
61993091 |
+ |
TAF4 |
NNNGAGCTGGACCCACCAAG |
NGG |
2 |
0.3618 |
Tier II |
| 5 |
NC_000018.10 |
18 |
36872419 |
+ |
KIAA1328 |
NNNTAGATGGACCCTCTAAG |
NGG |
2 |
0.35 |
Tier II |
| 6 |
NC_000001.11 |
1 |
48773427 |
+ |
BEND5 |
NNNTATGTGGACCCTCCAAG |
NGG |
2 |
0.3137 |
Tier II |
| 7 |
NC_000001.11 |
1 |
48773427 |
+ |
AGBL4 |
NNNTATGTGGACCCTCCAAG |
NGG |
2 |
0.3137 |
Tier II |
| 8 |
NC_000012.12 |
12 |
62894042 |
- |
PPM1H |
NNNTGGCTGGACCATCCAAG |
NGG |
2 |
0.252 |
Tier II |
| 9 |
NC_000005.10 |
5 |
10609058 |
+ |
ANKRD33B |
NNNTAGCTGGTACCTCCAAG |
NGG |
2 |
0.2198 |
Tier II |
| 10 |
NC_000015.10 |
15 |
66745119 |
+ |
SMAD6 |
NNNTAGCAGGACCCTCCAAG |
NTG |
2 |
0.0312 |
Tier II |
| 11 |
NC_000016.10 |
16 |
88488414 |
- |
ZFPM1 |
NNNTCGCTGGACCCGCCAAG |
NGG |
2 |
0.025 |
Tier II |
| 12 |
NC_000022.11 |
22 |
49538943 |
+ |
MIR3667HG |
NNNTAGCTGGACCCACCAAA |
NGG |
2 |
0.5428 |
Tier III |
| 13 |
NC_000009.12 |
9 |
99300103 |
+ |
LOC124902231 |
NNNTGGCTGGGCCCTCCAAG |
NGG |
2 |
0.468 |
Tier III |
| 14 |
NC_000012.12 |
12 |
115452945 |
+ |
LOC105370003 |
NNNTAGCTGGACCCCACAAG |
NGG |
2 |
0.2727 |
Tier III |
| 15 |
NC_000012.12 |
12 |
62894042 |
- |
LOC105369795 |
NNNTGGCTGGACCATCCAAG |
NGG |
2 |
0.252 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
97831775 |
- |
Mitf |
NNNCAGCTGGACCCTCCAAG |
NGG |
1 |
0.6471 |
Tier II |
| 2 |
NC_000070.6 |
4 |
127026483 |
+ |
Sfpq |
NNNTAGTTGGACCCTCCAAC |
NGG |
2 |
0.3482 |
Tier II |
| 3 |
NC_000079.6 |
13 |
115014112 |
+ |
Itga1 |
NNNTAGCTGGCCCCTCCTAG |
NGG |
2 |
0.2 |
Tier II |
| 4 |
NC_000072.6 |
6 |
5407817 |
- |
Asb4 |
NNNTAGCTGGCCCCTCCAGG |
NGG |
2 |
0.15 |
Tier II |
| 5 |
NC_000070.6 |
4 |
133220820 |
+ |
Cd164l2 |
NNNTACCTGGACCCTCCCAG |
NGG |
2 |
0.1299 |
Tier II |
| 6 |
NC_000073.6 |
7 |
27162940 |
+ |
Egln2 |
NNNTAGCTGGACCCTCCCTG |
NGG |
2 |
0.1026 |
Tier II |
| 7 |
NC_000067.6 |
1 |
182872169 |
+ |
Susd4 |
NNNTAGCTGGCCCCTGCAAG |
NGG |
2 |
0.0615 |
Tier II |
| 8 |
NC_000075.6 |
9 |
13611754 |
+ |
Maml2 |
NNNGAGCTGGACCCTCCAAG |
NGA |
2 |
0.0434 |
Tier II |
| 9 |
NC_000077.6 |
11 |
67490134 |
- |
Gas7 |
NNNTAGCTGGACCCTCCATG |
NGC |
2 |
0.012 |
Tier II |
| 10 |
NC_000067.6 |
1 |
32582418 |
+ |
Khdrbs2 |
NNNTAGCTGGACCCTTCAAG |
NTG |
2 |
0.012 |
Tier II |
| 11 |
NC_000076.6 |
10 |
91413648 |
- |
Gm40736 |
NNNTAGTTGGACCCTCAAAG |
NGG |
2 |
0.3792 |
Tier III |
| 12 |
NC_000074.6 |
8 |
114041080 |
+ |
Gm30107 |
NNNTTTCTGGACCCTCCAAG |
NGG |
2 |
0.2424 |
Tier III |
| 13 |
NC_000070.6 |
4 |
118987349 |
- |
Gm12864 |
NNNTGGCTGGACCCTTCAAG |
NGG |
2 |
0.2215 |
Tier III |
| 14 |
NC_000070.6 |
4 |
118987349 |
- |
Gm12865 |
NNNTGGCTGGACCCTTCAAG |
NGG |
2 |
0.2215 |
Tier III |
Other clones with same target sequence:
(none)
