Construct: sgRNA BRDN0001149471
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGCGAAGCTAAAACACTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ERN1 (2081)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76410
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
64054392 |
+ |
ERN1 |
NNNCGAAGCTAAAACACTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
129022029 |
- |
SH3GLB2 |
NNNGGAAGCTAAAACACACG |
NGG |
2 |
0.3333 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
106408202 |
+ |
Ern1 |
NNNCAAAGCTAAAACACTCA |
NGG |
2 |
0.8125 |
Tier I |
| 2 |
NC_000082.6 |
16 |
85899681 |
+ |
Adamts5 |
NNNCGAAGCTAAAGCCCTCG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000073.6 |
7 |
35381848 |
- |
Rhpn2 |
NNNTGAGGCTAAAACACTCG |
NGG |
2 |
0.5647 |
Tier II |
| 4 |
NC_000067.6 |
1 |
60482465 |
+ |
Raph1 |
NNNCTAAACTAAAACACTCG |
NGG |
2 |
0.3 |
Tier II |
| 5 |
NC_000081.6 |
15 |
55041904 |
- |
Taf2 |
NNNCAAAGCTAAAACAGTCG |
NGG |
2 |
0.051 |
Tier II |
| 6 |
NC_000079.6 |
13 |
53395222 |
- |
Gm2762 |
NNNCGAAGCTAAAACACTCT |
NGG |
1 |
0.7 |
Tier III |
Other clones with same target sequence:
(none)
