Construct: sgRNA BRDN0001149473

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTGTGTTTGCTAAATCCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BTK (695)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77074

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 101358622 - BTK NNNTGTTTGCTAAATCCACA NGG 0 1.0 Tier I
2 NC_000003.12 3 185930994 - TRA2B NNNTATTTGCTAAATACACA NGG 2 0.8667 Tier II
3 NC_000002.12 2 20671665 - GDF7 NNNTGTTTGCAAAATACACA NGG 2 0.75 Tier II
4 NC_000009.12 9 614762 - KANK1 NNNTGTTCACTAAATCCACA NGG 2 0.6429 Tier II
5 NC_000005.10 5 34001901 - AMACR NNNTGTTTGCTAATTACACA NGG 2 0.5333 Tier II
6 NC_000013.11 13 26267911 + CDK8 NNNCCTTTGCTAAATCCACA NGG 2 0.5084 Tier II
7 NC_000023.11 X 28625484 - IL1RAPL1 NNNTGTTTGCTAAATACAAA NGG 2 0.4286 Tier II
8 NC_000003.12 3 9303573 - SRGAP3 NNNTGTTAGCTAAATCAACA NGG 2 0.3733 Tier II
9 NC_000002.12 2 9304400 + ASAP2 NNNTGTTTGCCAAATCTACA NGG 2 0.3111 Tier II
10 NC_000005.10 5 115812125 + CDO1 NNNTGTTTCCTAAATCCACA NAG 2 0.1396 Tier II
11 NC_000006.12 6 87578580 + RARS2 NNNTGTTTACTAACTCCACA NGG 2 0.1378 Tier II
12 NC_000016.10 16 5505061 - RBFOX1 NNNTGTTTGGTATATCCACA NGG 2 0.1167 Tier II
13 NC_000012.12 12 65954700 + HMGA2 NNNTGTTTGCGAAATCCACC NGG 2 0.0909 Tier II
14 NC_000002.12 2 25829506 - ASXL2 NNNTGTTTGCTAAATCAACA NGA 2 0.0324 Tier II
15 NC_000002.12 2 102198061 + IL1RL2 NNNTGTTTGCTAAATCCAGA NAG 2 0.0324 Tier II
16 NC_000018.10 18 63965148 - HMSD NNNTGTTTGCTAGATCCACA NTG 2 0.0254 Tier II
17 NC_000008.11 8 140439617 - TRAPPC9 NNNTGTTTGCTAAAGCCAAA NGG 2 0.0214 Tier II
18 NC_000013.11 13 108921062 - MYO16 NNNTGTTTGCTACAGCCACA NGG 2 0.0105 Tier II
19 NC_000001.11 1 42155309 - GUCA2B NNNTGTTTGCTAAATGGACA NGG 2 0.009 Tier II
20 NC_000013.11 13 110901481 - ANKRD10 NNNTGTTTGCTAAATCCAAA NGT 2 0.0069 Tier II
21 NC_000023.11 X 132730370 + HS6ST2 NNNTGTTTGCTAAAGCGACA NGG 2 0.0029 Tier II
22 NC_000005.10 5 34001901 - C1QTNF3-AMACR NNNTGTTTGCTAATTACACA NGG 2 0.5333 Tier III
23 NC_000006.12 6 26479332 - LOC285819 NNNGGGTTGCTAAATCCACA NGG 2 0.3571 Tier III
24 NC_000009.12 9 12771384 + LURAP1L-AS1 NNNTGTCTGCTAAATCCTCA NGG 2 0.3438 Tier III
25 NC_000008.11 8 56642544 + LOC105375851 NNNTGTTTGTTTAATCCACA NGG 2 0.3137 Tier III
26 NC_000008.11 8 129227608 + LINC00977 NNNTGTTTCCTATATCCACA NGG 2 0.1615 Tier III
27 NC_000012.12 12 93075475 - LOC643339 NNNTGTTTGCTAAATCAACA NGA 2 0.0324 Tier III
28 NC_000006.12 6 22659153 + LINC03005 NNNTGTTGGCTAAATCCACA NGC 2 0.0163 Tier III
29 NC_000006.12 6 22659153 + LOC105374973 NNNTGTTGGCTAAATCCACA NGC 2 0.0163 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 134554694 - Btk NNNTGTTTGCTAAATCTACT NGG 2 0.28 Tier I
2 NC_000074.6 8 19185762 + Defb14 NNNTATTTGATAAATCCACA NGG 2 0.7511 Tier II
3 NC_000067.6 1 180449940 - Stum NNNTGTTAGCTGAATCCACA NGG 2 0.5778 Tier II
4 NC_000071.6 5 127279338 - Tmem132c NNNTGTTGGCTAAAACCACA NGG 2 0.4246 Tier II
5 NC_000079.6 13 64077172 + Hsd17b3 NNNTGTTTGCAAATTCCACA NGG 2 0.4 Tier II
6 NC_000082.6 16 25411539 - Tprg NNNTGTTTGCTGAATCTACA NGG 2 0.337 Tier II
7 NC_000070.6 4 73736758 + Rasef NNNTCTTTGCTTAATCCACA NGG 2 0.2619 Tier II
8 NC_000079.6 13 103118110 + Mast4 NNNTGTTTGGTAAATCCTCA NGG 2 0.1944 Tier II
9 NC_000070.6 4 139266680 - Capzb NNNTGTGTGCTAAATCCCCA NGG 2 0.112 Tier II
10 NC_000086.7 X 36752885 + Slc25a43 NNNTGTTTGCCAAATCCAGA NGG 2 0.0833 Tier II
11 NC_000076.6 10 121454966 - Rassf3 NNNTGTTTGCTTCATCCACA NGG 2 0.0702 Tier II
12 NC_000076.6 10 108171726 + Ppp1r12a NNNTGTTTGCTAAATCCACC NAG 2 0.0589 Tier II
13 NC_000069.6 3 152193992 - Dnajb4 NNNGGTTTGCTAAATCCACA NGA 2 0.0434 Tier II
14 NC_000071.6 5 96626601 - Fras1 NNNTGTGTGCTAAAGCCACA NGG 2 0.0294 Tier II
15 NC_000079.6 13 8402124 + Adarb2 NNNTGTTTGCTGAATCCACA NTG 2 0.0281 Tier II
16 NC_000074.6 8 99412377 + Cdh8 NNNTGTTTGCGAAATCCACA NGA 2 0.0278 Tier II
17 NC_000074.6 8 88660121 + Nod2 NNNTGTTTGCTAAATGCAGA NGG 2 0.0192 Tier II
18 NC_000071.6 5 49068882 + Kcnip4 NNNTGTTTGCTACATCCACA NGA 2 0.0146 Tier II
19 NC_000070.6 4 116555314 - Tmem69 NNNTGTTTGCTAAATCCAAA NGT 2 0.0069 Tier II
20 NC_000084.6 18 80318444 - Kcng2 NNNTGTTTGCTAAATCCCCA NGT 2 0.0031 Tier II
21 NC_000067.6 1 126156997 + Nckap5 NNNTGTTTGCTAAAGCCACA NGT 2 0.0008 Tier II
22 NC_000078.6 12 21183493 + Asap2 NNNTGTTTGCTAAATCCACA NTA 2 0.0 Tier II
23 NC_000071.6 5 127279338 - Tmem132cos NNNTGTTGGCTAAAACCACA NGG 2 0.4246 Tier III
24 NC_000068.7 2 50789931 + Gm32232 NNNTGTATGCTTAATCCACA NGG 2 0.2917 Tier III
25 NC_000081.6 15 63354025 + Gm41335 NNNGGTTTGCTAAATCCACA NAG 2 0.162 Tier III
26 NC_000079.6 13 51258347 - Gm46414 NNNTGTTTGCTACATCAACA NGG 2 0.0982 Tier III
27 NC_000071.6 5 146812527 + 1700041I07Rik NNNTGTTTGCTAAGTGCACA NGG 2 0.0718 Tier III
28 NC_000074.6 8 128205079 + A930035F09Rik NNNTGTTTGCTAACTCCCCA NGG 2 0.0408 Tier III
29 NC_000084.6 18 56697621 + Gm41738 NNNTGTTTGCTAAATTCAGA NGG 2 0.0385 Tier III
30 NC_000074.6 8 80577386 - Gm46044 NNNTGTTTGCTAAATCCACC NGA 2 0.0158 Tier III
31 NC_000071.6 5 41609028 - Gm35380 NNNTGTTTGCTATATCCACA NTG 2 0.0117 Tier III
32 NC_000084.6 18 80318444 - Gm29913 NNNTGTTTGCTAAATCCCCA NGT 2 0.0031 Tier III
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Other clones with same target sequence:

(none)