Construct: sgRNA BRDN0001149474

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAATGCTACGTATCGATCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARK1 (4139)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77793

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 220650684 - MARK1 NNNTGCTACGTATCGATCTG NGG 0 1.0 Tier I
2 NC_000004.12 4 25027028 + LGI2 NNNTGCTACGTTTTGATCTG NGG 2 0.2444 Tier II
3 NC_000002.12 2 219529283 - ASIC4 NNNTGCTATGTATCCATCTG NGG 2 0.2386 Tier II
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 34863740 + Mylk NNNTTCTACATATCGATCTG NGG 2 0.28 Tier II
2 NC_000075.6 9 92348890 + 1700057G04Rik NNNTGCTACGTATCCATCTG NAG 2 0.0707 Tier II
Download CSV

Other clones with same target sequence:

(none)