Construct: sgRNA BRDN0001149475
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTGATGTGATCGTGACACT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TPK1 (27010)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77427
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
144591553 |
- |
TPK1 |
NNNGATGTGATCGTGACACT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
59035000 |
- |
PRELID3B |
NNNGAAGTGATCGTGACAGT |
NGG |
2 |
0.1083 |
Tier I |
| 3 |
NC_000016.10 |
16 |
6942695 |
+ |
RBFOX1 |
NNNGATGTGAACTTGACACT |
NGG |
2 |
0.225 |
Tier II |
| 4 |
NC_000007.14 |
7 |
154798640 |
+ |
DPP6 |
NNNGTTGTGATTGTGACACT |
NGG |
2 |
0.1958 |
Tier II |
| 5 |
NC_000007.14 |
7 |
155678379 |
+ |
RBM33 |
NNNAATGTGATCGTTACACT |
NGG |
2 |
0.1286 |
Tier II |
| 6 |
NC_000002.12 |
2 |
149331575 |
- |
LYPD6 |
NNNGATGTGATTGTGACACT |
NGA |
2 |
0.0374 |
Tier II |
| 7 |
NC_000012.12 |
12 |
123896455 |
+ |
DNAH10 |
NNNGATGTGATCTTGACACT |
NGA |
2 |
0.0208 |
Tier II |
| 8 |
NC_000011.10 |
11 |
64141768 |
+ |
MACROD1 |
NNNGATGTGCTCGGGACACT |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000014.9 |
14 |
101800209 |
+ |
PPP2R5C |
NNNGCTGTGATCGTGCCACT |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000016.10 |
16 |
69612835 |
+ |
NFAT5 |
NNNGCTGTGATCGTGTCACT |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000020.11 |
20 |
59035000 |
- |
SLMO2-ATP5E |
NNNGAAGTGATCGTGACAGT |
NGG |
2 |
0.1083 |
Tier III |
| 12 |
NC_000006.12 |
6 |
84497237 |
+ |
LOC107986620 |
NNNGATGTGATGGTGACACG |
NGG |
2 |
0.0784 |
Tier III |
| 13 |
NC_000018.10 |
18 |
71777911 |
- |
LINC01899 |
NNNGCTGTGATCGTGACACT |
NTG |
2 |
0.0195 |
Tier III |
| 14 |
NC_000018.10 |
18 |
71777911 |
- |
LOC105376873 |
NNNGCTGTGATCGTGACACT |
NTG |
2 |
0.0195 |
Tier III |
| 15 |
NC_000014.9 |
14 |
101800209 |
+ |
LOC124903388 |
NNNGCTGTGATCGTGCCACT |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
43469132 |
- |
Tpk1 |
NNNGACGTGATTGTGACACT |
NGG |
2 |
0.4895 |
Tier I |
| 2 |
NC_000071.6 |
5 |
89121664 |
- |
Slc4a4 |
NNNGATGTGATTGTGACATT |
NGG |
2 |
0.2485 |
Tier II |
| 3 |
NC_000072.6 |
6 |
43469132 |
- |
9430018G01Rik |
NNNGACGTGATTGTGACACT |
NGG |
2 |
0.4895 |
Tier III |
| 4 |
NC_000069.6 |
3 |
148340652 |
- |
Gm29836 |
NNNGATGTCATCGTTACACT |
NGG |
2 |
0.0769 |
Tier III |
| 5 |
NC_000083.6 |
17 |
6446011 |
- |
Tmem181b-ps |
NNNTATGTGATCGTGACACG |
NGG |
2 |
0.0642 |
Tier III |
Other clones with same target sequence:
(none)
