Construct: sgRNA BRDN0001149479
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAAAGACTTACCAGTATCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKG (1608)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77454
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
186297427 |
+ |
DGKG |
NNNAGACTTACCAGTATCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
17079615 |
+ |
MPRIP |
NNNTGACTTAACAGTATCAG |
NGG |
2 |
0.4773 |
Tier II |
| 3 |
NC_000001.11 |
1 |
179782504 |
- |
FAM163A |
NNNAGCCTTACCAGTACCAG |
NGG |
2 |
0.3209 |
Tier II |
| 4 |
NC_000003.12 |
3 |
196941717 |
- |
NCBP2 |
NNNAGACTTTTCAGTATCAG |
NGG |
2 |
0.2715 |
Tier II |
| 5 |
NC_000005.10 |
5 |
153615993 |
+ |
GRIA1 |
NNNAGACTTTGCAGTATCAG |
NGG |
2 |
0.2206 |
Tier II |
| 6 |
NC_000023.11 |
X |
69958811 |
- |
EDA |
NNNAGACTTACATGTATCAG |
NGG |
2 |
0.2143 |
Tier II |
| 7 |
NC_000018.10 |
18 |
47897738 |
- |
SMAD2 |
NNNGGACTTACCAGTATCAG |
NAG |
2 |
0.162 |
Tier II |
| 8 |
NC_000003.12 |
3 |
38751492 |
+ |
SCN10A |
NNNAGACTTATCTGTATCAG |
NGG |
2 |
0.0923 |
Tier II |
| 9 |
NC_000005.10 |
5 |
111014122 |
- |
LOC105379121 |
NNNACACTTACCAATATCAG |
NGG |
2 |
0.5893 |
Tier III |
| 10 |
NC_000003.12 |
3 |
196941717 |
- |
NCBP2-AS1 |
NNNAGACTTTTCAGTATCAG |
NGG |
2 |
0.2715 |
Tier III |
| 11 |
NC_000010.11 |
10 |
64602423 |
+ |
LOC105378335 |
NNNAGACTTACCAGTATCAG |
NTT |
2 |
0.0 |
Tier III |
| 12 |
NC_000010.11 |
10 |
64602423 |
+ |
LOC124902439 |
NNNAGACTTACCAGTATCAG |
NTT |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
138308324 |
- |
Ddost |
NNNAGACTTACCAATATCAG |
NGC |
2 |
0.0167 |
Tier I |
| 2 |
NC_000071.6 |
5 |
138192673 |
- |
Cnpy4 |
NNNACACTTACCAGTTTCAG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000073.6 |
7 |
119958369 |
+ |
Dnah3 |
NNNAGACCTACCAGAATCAG |
NGG |
2 |
0.5789 |
Tier II |
| 4 |
NC_000069.6 |
3 |
156796087 |
+ |
Negr1 |
NNNAAACTTACCATTATCAG |
NGG |
2 |
0.2311 |
Tier II |
| 5 |
NC_000079.6 |
13 |
36962089 |
+ |
F13a1 |
NNNAGATTTACCAGTATCAG |
NAG |
2 |
0.2106 |
Tier II |
| 6 |
NC_000076.6 |
10 |
31428418 |
- |
Tpd52l1 |
NNNAGACTTCCCAGTTTCAG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000079.6 |
13 |
36962089 |
+ |
Gm30489 |
NNNAGATTTACCAGTATCAG |
NAG |
2 |
0.2106 |
Tier III |
| 8 |
NC_000075.6 |
9 |
61805408 |
- |
Gm34828 |
NNNAGACTTACCAGCATCAC |
NGG |
2 |
0.1169 |
Tier III |
| 9 |
NC_000082.6 |
16 |
78070472 |
- |
Gm31431 |
NNNAGTCTTACCAGTATCAG |
NGA |
2 |
0.0496 |
Tier III |
Other clones with same target sequence:
(none)
