Construct: sgRNA BRDN0001149487
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCTGAGGGTTTACCCCGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CHKB (1120)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76864
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
50582563 |
- |
CHKB |
NNNTGAGGGTTTACCCCGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
139802650 |
+ |
PSD2 |
NNNTGAGGGTTCACCCCGTA |
NGG |
2 |
0.8882 |
Tier II |
| 3 |
NC_000011.10 |
11 |
62836310 |
+ |
WDR74 |
NNNTGAGGGTTTACCAAGTG |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000023.11 |
X |
150981227 |
+ |
HMGB3 |
NNNTGAGGGATTACCCTGTG |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000009.12 |
9 |
70588567 |
- |
TRPM3 |
NNNTGTGGGTGTACCCCGTG |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000006.12 |
6 |
63883459 |
- |
EYS |
NNNTGAGAGTTTCCCCCGTG |
NGG |
2 |
0.2105 |
Tier II |
| 7 |
NC_000022.11 |
22 |
50582563 |
- |
CHKB-CPT1B |
NNNTGAGGGTTTACCCCGTG |
NGG |
0 |
1.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
144170925 |
- |
Sox5 |
NNNTGAGGGTTTACCCCATT |
NGG |
2 |
0.4846 |
Tier II |
| 2 |
NC_000071.6 |
5 |
135315468 |
- |
Fkbp6 |
NNNTGAGGGCTTACCCCATG |
NGG |
2 |
0.3692 |
Tier II |
| 3 |
NC_000079.6 |
13 |
71775540 |
- |
Gm40999 |
NNNTGAGGGTTTCCCCCGAG |
NGG |
2 |
0.0602 |
Tier III |
Other clones with same target sequence:
(none)
