Construct: sgRNA BRDN0001149493
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CACACTTACATCATCGGCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MPP7 (143098)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75834
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
28149990 |
+ |
MPP7 |
NNNACTTACATCATCGGCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
634492 |
- |
DIP2C |
NNNACTGACATCATCAGCCA |
NGG |
2 |
0.5882 |
Tier II |
| 3 |
NC_000010.11 |
10 |
634492 |
- |
LOC124902361 |
NNNACTGACATCATCAGCCA |
NGG |
2 |
0.5882 |
Tier II |
| 4 |
NC_000019.10 |
19 |
17110431 |
+ |
MYO9B |
NNNCCTTACAGCATCGGCCA |
NGG |
2 |
0.1412 |
Tier II |
| 5 |
NC_000012.12 |
12 |
1454104 |
- |
ERC1 |
NNNAATTACATCATTGGCCA |
NGG |
2 |
0.0381 |
Tier II |
| 6 |
NC_000003.12 |
3 |
196883539 |
+ |
SENP5 |
NNNACTTTCATCATCTGCCA |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000003.12 |
3 |
184508284 |
+ |
LINC01839 |
NNNACCTACATCAACGGCCA |
NGG |
2 |
0.5628 |
Tier III |
| 8 |
NC_000012.12 |
12 |
1454104 |
- |
LOC124902856 |
NNNAATTACATCATTGGCCA |
NGG |
2 |
0.0381 |
Tier III |
| 9 |
NC_000002.12 |
2 |
220843124 |
+ |
LOC107985990 |
NNNACTTACATCATCTGCCA |
NAG |
2 |
0.0 |
Tier III |
| 10 |
NC_000023.11 |
X |
23062918 |
- |
PTCHD1-AS |
NNNACTTACCTCATCTGCCA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
8231826 |
+ |
Acox2 |
NNNACTCACATCATCAGCCA |
NGG |
2 |
0.6875 |
Tier II |
| 2 |
NC_000068.7 |
2 |
127340816 |
- |
Astl |
NNNACTGACATGATCGGCCA |
NGG |
2 |
0.2614 |
Tier II |
| 3 |
NC_000079.6 |
13 |
36174850 |
- |
Fars2 |
NNNAGTTACATCATCGGCCC |
NGG |
2 |
0.1364 |
Tier II |
| 4 |
NC_000081.6 |
15 |
39629594 |
- |
Rims2 |
NNNACTTACGTCATTGGCCA |
NGG |
2 |
0.037 |
Tier II |
| 5 |
NC_000080.6 |
14 |
62892421 |
- |
Wdfy2 |
NNNACTTACATCAGTGGCCA |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
