Construct: sgRNA BRDN0001149495
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCATGAGGTAATATACGATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PFKFB4 (5210)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77123
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
48536383 |
+ |
PFKFB4 |
NNNTGAGGTAATATACGATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
206593479 |
+ |
ADAM23 |
NNNTGAGGTAATATAAGATT |
NGG |
2 |
0.7 |
Tier II |
| 3 |
NC_000010.11 |
10 |
126279088 |
+ |
ADAM12 |
NNNTGAGGGAATAAACGATG |
NGG |
2 |
0.3832 |
Tier II |
| 4 |
NC_000013.11 |
13 |
93422557 |
+ |
GPC6 |
NNNTGAGGTAATATATGGTG |
NGG |
2 |
0.1231 |
Tier II |
| 5 |
NC_000015.10 |
15 |
54318443 |
+ |
UNC13C |
NNNTGAGGTAATATCTGATG |
NGG |
2 |
0.0839 |
Tier II |
| 6 |
NC_000004.12 |
4 |
13424482 |
- |
RAB28 |
NNNTGAGGTAATATTTGATG |
NGG |
2 |
0.0615 |
Tier II |
| 7 |
NC_000003.12 |
3 |
71109725 |
+ |
FOXP1 |
NNNTGAGGTAATATACCATG |
NGA |
2 |
0.0163 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
5027413 |
- |
Slc29a2 |
NNNTGAGGTAACATACGATG |
NAG |
2 |
0.2456 |
Tier I |
| 2 |
NC_000075.6 |
9 |
109010530 |
- |
Pfkfb4 |
NNNTGAGGTAATAAACGATG |
NGA |
2 |
0.043 |
Tier I |
| 3 |
NC_000079.6 |
13 |
37389439 |
- |
Ly86 |
NNNTGAGGTAAGATAAGATG |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000082.6 |
16 |
56534670 |
- |
Abi3bp |
NNNTAAGGTAATATATGATG |
NGG |
2 |
0.2667 |
Tier II |
| 5 |
NC_000078.6 |
12 |
40084783 |
+ |
Scin |
NNNTGAGGTAAAATATGATG |
NGG |
2 |
0.2462 |
Tier II |
| 6 |
NC_000084.6 |
18 |
58927167 |
+ |
Adamts19 |
NNNTGAGGGAATATATGATG |
NGG |
2 |
0.1905 |
Tier II |
Other clones with same target sequence:
(none)
