Construct: sgRNA BRDN0001149496
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTCAACCAAAATACACCCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DYRK1A (1859)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76753
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000021.9 |
21 |
37493108 |
- |
DYRK1A |
NNNAACCAAAATACACCCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
28426147 |
+ |
EIF3CL |
NNNAACCAAAAAACACCCAA |
NGG |
2 |
0.5714 |
Tier II |
| 3 |
NC_000016.10 |
16 |
28688866 |
- |
EIF3C |
NNNAACCAAAAAACACCCAA |
NGG |
2 |
0.5714 |
Tier II |
| 4 |
NC_000001.11 |
1 |
13782891 |
- |
PRDM2 |
NNNAACCAAAAAACACCCGT |
NGG |
2 |
0.48 |
Tier II |
| 5 |
NC_000001.11 |
1 |
215232829 |
+ |
KCNK2 |
NNNAACCAAAATAAACCTGA |
NGG |
2 |
0.225 |
Tier II |
| 6 |
NC_000009.12 |
9 |
135898134 |
+ |
CAMSAP1 |
NNNGACCAAAATAAACCCGA |
NGG |
2 |
0.2188 |
Tier II |
| 7 |
NC_000018.10 |
18 |
3451343 |
+ |
TGIF1 |
NNNAAACAAAATACACCGGA |
NGG |
2 |
0.1238 |
Tier II |
| 8 |
NC_000001.11 |
1 |
203086244 |
- |
MYOPARR |
NNNAATCAAATTACACCCGA |
NGG |
2 |
0.2857 |
Tier III |
| 9 |
NC_000002.12 |
2 |
122886961 |
- |
LOC105373595 |
NNNAACCAAAGTACTCCCGA |
NGG |
2 |
0.13 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
28853505 |
- |
Ddx31 |
NNNAACTAAAACACACCCGA |
NGG |
2 |
0.7697 |
Tier II |
| 2 |
NC_000072.6 |
6 |
137274258 |
- |
Ptpro |
NNNAACTAAAACACACCCGA |
NGG |
2 |
0.7697 |
Tier II |
| 3 |
NC_000082.6 |
16 |
30262012 |
- |
Cpn2 |
NNNAAACAAAATGCACCCGA |
NGG |
2 |
0.6056 |
Tier II |
| 4 |
NC_000078.6 |
12 |
59016991 |
- |
Gemin2 |
NNNAACAGAAATACACCCGA |
NGG |
2 |
0.55 |
Tier II |
| 5 |
NC_000072.6 |
6 |
67298598 |
+ |
Il12rb2 |
NNNAACCAAAAAACACCCTA |
NGG |
2 |
0.5333 |
Tier II |
| 6 |
NC_000086.7 |
X |
153379315 |
+ |
Klf8 |
NNNAACCAAAAAACACCCTA |
NGG |
2 |
0.5333 |
Tier II |
| 7 |
NC_000068.7 |
2 |
7373101 |
+ |
Celf2 |
NNNAACCAAAAAACACCTGA |
NGG |
2 |
0.5143 |
Tier II |
| 8 |
NC_000071.6 |
5 |
141852695 |
+ |
Sdk1 |
NNNAACCAAAACACACCCCA |
NGG |
2 |
0.4247 |
Tier II |
| 9 |
NC_000076.6 |
10 |
58474764 |
- |
Ranbp2 |
NNNAACCAAAATGCACCTGA |
NGG |
2 |
0.4193 |
Tier II |
| 10 |
NC_000083.6 |
17 |
50139195 |
- |
Rftn1 |
NNNGACCAAAATAAACCCGA |
NGG |
2 |
0.2188 |
Tier II |
| 11 |
NC_000072.6 |
6 |
93972864 |
+ |
Magi1 |
NNNAACCAAACTACACCCCA |
NGG |
2 |
0.1793 |
Tier II |
| 12 |
NC_000084.6 |
18 |
49738125 |
- |
Dtwd2 |
NNNAACCAAAATACAGCAGA |
NGG |
2 |
0.0828 |
Tier II |
| 13 |
NC_000073.6 |
7 |
81923175 |
+ |
Hdgfl3 |
NNNAACCAAAATACACCCTA |
NGT |
2 |
0.0108 |
Tier II |
| 14 |
NC_000072.6 |
6 |
137274258 |
- |
Gm52924 |
NNNAACTAAAACACACCCGA |
NGG |
2 |
0.7697 |
Tier III |
| 15 |
NC_000070.6 |
4 |
120216256 |
+ |
Gm36134 |
NNNAACCACAAGACACCCGA |
NGG |
2 |
0.2857 |
Tier III |
| 16 |
NC_000077.6 |
11 |
4290839 |
- |
Gm11958 |
NNNAGCCAAAATACACCCGC |
NGG |
2 |
0.1636 |
Tier III |
Other clones with same target sequence:
(none)
