Construct: sgRNA BRDN0001149502
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGAAGAAACTCGACGATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SH3BP4 (23677)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78088
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
235041553 |
- |
SH3BP4 |
NNNAAGAAACTCGACGATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
42407154 |
- |
LYZL4 |
NNNAAGAAACTCCACGATGG |
NGG |
2 |
0.0743 |
Tier I |
| 3 |
NC_000001.11 |
1 |
117883503 |
- |
GDAP2 |
NNNAAGAAACTCTACGATGT |
NGT |
2 |
0.0048 |
Tier I |
| 4 |
NC_000015.10 |
15 |
65869903 |
- |
RAB11A |
NNNAAGGAATTCGACGATGT |
NGG |
2 |
0.6644 |
Tier II |
| 5 |
NC_000011.10 |
11 |
16306928 |
- |
SOX6 |
NNNAAGAAATTCGAGGATGT |
NGG |
2 |
0.0471 |
Tier II |
| 6 |
NC_000002.12 |
2 |
137631793 |
+ |
THSD7B |
NNNAAGAAACTCGACGATCT |
NTG |
2 |
0.0175 |
Tier II |
| 7 |
NC_000011.10 |
11 |
112461588 |
- |
RPL23AP62 |
NNNAAGAAACTCTACGACGT |
NGG |
2 |
0.1286 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
100194615 |
+ |
Gdap2 |
NNNAAGAAACTCTACGATGT |
NGT |
2 |
0.0048 |
Tier I |
| 2 |
NC_000082.6 |
16 |
15908771 |
- |
Spidr |
NNNAAGAAACTAGAAGATGT |
NGG |
2 |
0.1587 |
Tier II |
| 3 |
NC_000069.6 |
3 |
107727083 |
- |
Gm40121 |
NNNTAGAAACTCTACGATGT |
NGG |
2 |
0.1909 |
Tier III |
Other clones with same target sequence:
(none)
