Construct: sgRNA BRDN0001149504
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAGCCTTATGATCGAGCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SGK2 (10110)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76208
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
43574952 |
+ |
SGK2 |
NNNGCCTTATGATCGAGCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
88173490 |
- |
NTRK3 |
NNNGCCTGATGATCGAGCAG |
NTG |
2 |
0.0286 |
Tier II |
| 3 |
NC_000001.11 |
1 |
24311186 |
- |
GRHL3-AS1 |
NNNGCCTTATGGTCCAGCAG |
NGG |
2 |
0.197 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
163003315 |
+ |
Sgk2 |
NNNGCCTTACGATCGAGCAG |
NGG |
1 |
0.5333 |
Tier I |
| 2 |
NC_000071.6 |
5 |
36090803 |
- |
Sorcs2 |
NNNGCCTCATGCTCGAGCAG |
NGG |
2 |
0.2632 |
Tier II |
| 3 |
NC_000073.6 |
7 |
29852233 |
- |
Zfp940 |
NNNGCCTCATGCTCGAGCAG |
NGG |
2 |
0.2632 |
Tier II |
| 4 |
NC_000070.6 |
4 |
49121133 |
- |
Plppr1 |
NNNGCCTTATGAGTGAGCAG |
NGG |
2 |
0.1913 |
Tier II |
| 5 |
NC_000078.6 |
12 |
102486909 |
- |
Golga5 |
NNNGCCTTATGATTGAGCAG |
NAG |
2 |
0.1901 |
Tier II |
| 6 |
NC_000071.6 |
5 |
120496552 |
+ |
Plbd2 |
NNNGCCTCATGATCTAGCAG |
NGG |
2 |
0.1429 |
Tier II |
| 7 |
NC_000074.6 |
8 |
22670252 |
+ |
Ikbkb |
NNNGCCTCATGATCTAGCAG |
NGG |
2 |
0.1429 |
Tier II |
| 8 |
NC_000074.6 |
8 |
94460920 |
- |
Nlrc5 |
NNNGCCTCATGATCTAGCAG |
NGG |
2 |
0.1429 |
Tier II |
| 9 |
NC_000076.6 |
10 |
81665904 |
+ |
Gm10778 |
NNNGCCTTATGGTCTAGCAG |
NGG |
2 |
0.1032 |
Tier II |
| 10 |
NC_000071.6 |
5 |
116074966 |
+ |
Tmem233 |
NNNGCCTTGTGATCTAGCAG |
NGG |
2 |
0.0952 |
Tier II |
| 11 |
NC_000068.7 |
2 |
18966581 |
- |
Pip4k2a |
NNNGCCTTATGATCGATCAG |
NAG |
2 |
0.0648 |
Tier II |
| 12 |
NC_000074.6 |
8 |
72710058 |
+ |
Nwd1 |
NNNGCCTTATGCTCTAGCAG |
NGG |
2 |
0.0376 |
Tier II |
| 13 |
NC_000077.6 |
11 |
3778654 |
- |
Osbp2 |
NNNGCCTTATGCTCTAGCAG |
NGG |
2 |
0.0376 |
Tier II |
| 14 |
NC_000072.6 |
6 |
31675162 |
- |
Gm13847 |
NNNGCCTTGTGATCTAGCAG |
NGG |
2 |
0.0952 |
Tier III |
| 15 |
NC_000072.6 |
6 |
31675162 |
- |
Gm13846 |
NNNGCCTTGTGATCTAGCAG |
NGG |
2 |
0.0952 |
Tier III |
| 16 |
NC_000074.6 |
8 |
80336007 |
+ |
Gm30605 |
NNNGCCTTATGCTCTAGCAG |
NGG |
2 |
0.0376 |
Tier III |
| 17 |
NC_000077.6 |
11 |
3778654 |
- |
Gm51882 |
NNNGCCTTATGCTCTAGCAG |
NGG |
2 |
0.0376 |
Tier III |
Other clones with same target sequence:
(none)
