Construct: sgRNA BRDN0001149508
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGTGTTCAACTAGTGCACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SMG1 (23049)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75772
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
18870679 |
- |
SMG1 |
NNNGTTCAACTAGTGCACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
71878194 |
+ |
MYO9A |
NNNGTTCAAATAGTGCATAG |
NGG |
2 |
0.5571 |
Tier I |
| 3 |
NC_000016.10 |
16 |
29438011 |
- |
SMG1P6 |
NNNGTTCAACTAGTGCACTG |
NGG |
1 |
0.5385 |
Tier III |
| 4 |
NC_000016.10 |
16 |
21890081 |
- |
SMG1P4 |
NNNGTTCAACTAGTGCACCG |
NGG |
1 |
0.2069 |
Tier III |
| 5 |
NC_000016.10 |
16 |
70233493 |
- |
SMG1P7 |
NNNGTTCAACTAGTGCACCG |
NGG |
1 |
0.2069 |
Tier III |
| 6 |
NC_000016.10 |
16 |
21457840 |
- |
SMG1P3 |
NNNGTCCAACTAGTGCACCG |
NGG |
2 |
0.1881 |
Tier III |
| 7 |
NC_000016.10 |
16 |
22481064 |
+ |
SMG1P1 |
NNNGTCCAACTAGTGCACCG |
NGG |
2 |
0.1881 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
8244474 |
- |
Adam22 |
NNNGTTCAAATAGTGCAAAG |
NGG |
2 |
0.4667 |
Tier II |
| 2 |
NC_000080.6 |
14 |
27995582 |
- |
Erc2 |
NNNGTTCAACTAGGCCACAG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
