Construct: sgRNA BRDN0001149510
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATTGTCCTTTGAACCAACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ACVR1C (130399)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76524
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
157550382 |
+ |
ACVR1C |
NNNGTCCTTTGAACCAACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
21862958 |
- |
ABCC9 |
NNNGTCCTTTTAACAAACAG |
NGG |
2 |
0.0855 |
Tier I |
| 3 |
NC_000008.11 |
8 |
32648223 |
+ |
NRG1 |
NNNCTCCTTTGAACCATCAG |
NGG |
2 |
0.0706 |
Tier I |
| 4 |
NC_000014.9 |
14 |
94043968 |
- |
OTUB2 |
NNNGTTCTTTGAACCTACAG |
NGG |
2 |
0.0 |
Tier I |
| 5 |
NC_000006.12 |
6 |
168011567 |
- |
KIF25 |
NNNGTCCATTAAACCAACAG |
NGG |
2 |
0.8 |
Tier II |
| 6 |
NC_000011.10 |
11 |
46942948 |
- |
CSTPP1 |
NNNGCCCTTTGAACCAATAG |
NGG |
2 |
0.6429 |
Tier II |
| 7 |
NC_000004.12 |
4 |
38986488 |
- |
TMEM156 |
NNNATCCTTTGAACCAACAT |
NGG |
2 |
0.63 |
Tier II |
| 8 |
NC_000001.11 |
1 |
214446079 |
+ |
PTPN14 |
NNNGTCATTTTAACCAACAG |
NGG |
2 |
0.2885 |
Tier II |
| 9 |
NC_000019.10 |
19 |
54045112 |
+ |
VSTM1 |
NNNGTCCTCTGCACCAACAG |
NGG |
2 |
0.2429 |
Tier II |
| 10 |
NC_000017.11 |
17 |
10505649 |
- |
MYH1 |
NNNGTCCTTTGAATAAACAG |
NGG |
2 |
0.163 |
Tier II |
| 11 |
NC_000013.11 |
13 |
46036518 |
+ |
ZC3H13 |
NNNGTCCTTTGAAAAAACAG |
NGG |
2 |
0.0778 |
Tier II |
| 12 |
NC_000020.11 |
20 |
47076014 |
- |
EYA2 |
NNNGTCCTTTGAACCAACAT |
NGA |
2 |
0.0486 |
Tier II |
| 13 |
NC_000013.11 |
13 |
92491883 |
- |
GPC5 |
NNNCTCCTTTGAACCAACAG |
NGA |
2 |
0.0368 |
Tier II |
| 14 |
NC_000002.12 |
2 |
189374115 |
- |
COL5A2 |
NNNGTCCTTTGAACCAACAC |
NGT |
2 |
0.0069 |
Tier II |
| 15 |
NC_000002.12 |
2 |
166102902 |
+ |
SCN1A |
NNNGTCCTTTGAAGCAACAT |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000003.12 |
3 |
97427772 |
+ |
EPHA6 |
NNNGTCCTTTGCAGCAACAG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000017.11 |
17 |
32753127 |
+ |
MYO1D |
NNNGTCCTTTGCAGCAACAG |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000001.11 |
1 |
42272108 |
+ |
FOXJ3 |
NNNGTCCTTTTAAGCAACAG |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000021.9 |
21 |
38164821 |
+ |
LOC124905020 |
NNNGTCCTTTGCACCAACAT |
NGG |
2 |
0.1842 |
Tier III |
| 20 |
NC_000017.11 |
17 |
10505649 |
- |
MYHAS |
NNNGTCCTTTGAATAAACAG |
NGG |
2 |
0.163 |
Tier III |
| 21 |
NC_000008.11 |
8 |
6669434 |
+ |
MCPH1-AS1 |
NNNGTCCTTTGAGCAAACAG |
NGG |
2 |
0.1449 |
Tier III |
| 22 |
NC_000013.11 |
13 |
92491883 |
- |
GPC5-IT1 |
NNNCTCCTTTGAACCAACAG |
NGA |
2 |
0.0368 |
Tier III |
| 23 |
NC_000002.12 |
2 |
166102902 |
+ |
SCN1A-AS1 |
NNNGTCCTTTGAAGCAACAT |
NGG |
2 |
0.0 |
Tier III |
| 24 |
NC_000011.10 |
11 |
19250972 |
+ |
CSRP3-AS1 |
NNNGTCCTTTGAAGCAACAT |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
58287901 |
+ |
Acvr1c |
NNNGTTCTTTGAACCAAGAG |
NGG |
2 |
0.1238 |
Tier I |
| 2 |
NC_000077.6 |
11 |
116769254 |
+ |
St6galnac1 |
NNNGTCCTTTGAACTGACAG |
NGG |
2 |
0.0128 |
Tier I |
| 3 |
NC_000067.6 |
1 |
160604014 |
+ |
Rabgap1l |
NNNGTCCTCTAAACCAACAG |
NGG |
2 |
0.9231 |
Tier II |
| 4 |
NC_000074.6 |
8 |
67861050 |
+ |
Psd3 |
NNNGTGCTTTGAGCCAACAG |
NGG |
2 |
0.3261 |
Tier II |
| 5 |
NC_000074.6 |
8 |
115014208 |
- |
Wwox |
NNNGTCCTCTGAAACAACAG |
NGG |
2 |
0.3231 |
Tier II |
| 6 |
NC_000084.6 |
18 |
44623579 |
+ |
Mcc |
NNNGTCCTTTGAATCAACAC |
NGG |
2 |
0.3143 |
Tier II |
| 7 |
NC_000069.6 |
3 |
109598841 |
- |
Vav3 |
NNNGTCTTTTGATCCAACAG |
NGG |
2 |
0.2438 |
Tier II |
| 8 |
NC_000068.7 |
2 |
163393418 |
+ |
Jph2 |
NNNGTCCTTTCAACCAAAAG |
NGG |
2 |
0.2308 |
Tier II |
| 9 |
NC_000075.6 |
9 |
36913620 |
- |
Pknox2 |
NNNCTCCTTTGAACCAACAC |
NGG |
2 |
0.2269 |
Tier II |
| 10 |
NC_000075.6 |
9 |
9947589 |
- |
Cntn5 |
NNNGTTCTTTGAACCAAGAG |
NGG |
2 |
0.1238 |
Tier II |
| 11 |
NC_000077.6 |
11 |
98506318 |
+ |
Ikzf3 |
NNNGTGCTTTGAACAAACAG |
NGG |
2 |
0.1111 |
Tier II |
| 12 |
NC_000083.6 |
17 |
3485352 |
- |
Tiam2 |
NNNGACCTTTGACCCAACAG |
NGG |
2 |
0.1053 |
Tier II |
| 13 |
NC_000078.6 |
12 |
55760312 |
- |
Ralgapa1 |
NNNGTCCTTTGAACCAGGAG |
NGG |
2 |
0.0235 |
Tier II |
| 14 |
NC_000071.6 |
5 |
25378236 |
- |
Kmt2c |
NNNGTTCTTTGAACCAACAG |
NGC |
2 |
0.0206 |
Tier II |
| 15 |
NC_000071.6 |
5 |
26239337 |
+ |
Gm7361 |
NNNGACCTTTGAAGCAACAG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000078.6 |
12 |
33358886 |
- |
Atxn7l1 |
NNNGGCCTTTGAAGCAACAG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000077.6 |
11 |
120036993 |
- |
Aatk |
NNNGTCCTTGGAACCCACAG |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000074.6 |
8 |
67861050 |
+ |
Gm32888 |
NNNGTGCTTTGAGCCAACAG |
NGG |
2 |
0.3261 |
Tier III |
| 19 |
NC_000070.6 |
4 |
120242814 |
+ |
Gm36134 |
NNNGTCCTTGGAACCAATAG |
NGG |
2 |
0.3214 |
Tier III |
| 20 |
NC_000067.6 |
1 |
71680187 |
+ |
Gm46094 |
NNNGTCATTTGAAACAACAG |
NGG |
2 |
0.2625 |
Tier III |
| 21 |
NC_000080.6 |
14 |
25257161 |
+ |
Zmiz1os1 |
NNNGTCATTTGAACAAACAG |
NGG |
2 |
0.1667 |
Tier III |
| 22 |
NC_000078.6 |
12 |
33358886 |
- |
Atxn7l1os2 |
NNNGGCCTTTGAAGCAACAG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
