Construct: sgRNA BRDN0001149511
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCACCCGTGTGATTCGCGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAK6 (56924)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76922
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
40264956 |
- |
PAK6 |
NNNCCCGTGTGATTCGCGAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
40264956 |
- |
BUB1B-PAK6 |
NNNCCCGTGTGATTCGCGAA |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000008.11 |
8 |
68521847 |
+ |
C8orf34 |
NNNCCCGTGTGATTGGAGAA |
NGG |
2 |
0.0233 |
Tier I |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
118688972 |
- |
Pak6 |
NNNCCCGTGTGATTCGAGAA |
NGA |
2 |
0.0324 |
Tier I |
| 2 |
NC_000074.6 |
8 |
89064654 |
+ |
Gm3134 |
NNNCCCGTGTGATTTGCTAA |
NGG |
2 |
0.0444 |
Tier III |
Other clones with same target sequence:
(none)
