Construct: sgRNA BRDN0001149512
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGCCCCAGGCGTAATCCTC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80223
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
2726280 |
+ |
CD99 |
NNNCCCCAGGCGTGATCCCC |
NGG |
2 |
0.1287 |
Tier I |
| 2 |
NC_000024.10 |
Y |
2726280 |
+ |
CD99 |
NNNCCCCAGGCGTGATCCCC |
NGG |
2 |
0.1287 |
Tier I |
| 3 |
NC_000006.12 |
6 |
167034201 |
- |
CEP43 |
NNNCCCCAGGAATAATCCTC |
NGG |
2 |
0.7 |
Tier II |
| 4 |
NC_000014.9 |
14 |
47072660 |
+ |
MDGA2 |
NNNCCCCAGGCCCAATCCTC |
NGG |
2 |
0.418 |
Tier II |
| 5 |
NC_000009.12 |
9 |
133401723 |
+ |
STKLD1 |
NNNCCCCAGGCGTCTTCCTC |
NGG |
2 |
0.0429 |
Tier II |
| 6 |
NC_000002.12 |
2 |
222302568 |
+ |
CCDC140 |
NNNCCCCAGGCGTGATTCTC |
NGG |
2 |
0.2178 |
Tier III |
| 7 |
NC_000010.11 |
10 |
9709717 |
+ |
LINC02663 |
NNNCCCCAGGCCTAATCCTC |
NGT |
2 |
0.0085 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
120476914 |
- |
Capn3 |
NNNGCCCAGGCCTAATCCTC |
NGG |
2 |
0.2647 |
Tier II |
| 2 |
NC_000078.6 |
12 |
89090491 |
- |
Nrxn3 |
NNNCCCCAGGCATAATCCTC |
NGT |
2 |
0.0151 |
Tier II |
Other clones with same target sequence:
(none)
