Construct: sgRNA BRDN0001149518
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCATCACCAAGTTCGCATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ATM (472)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77529
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
108272828 |
+ |
ATM |
NNNTCACCAAGTTCGCATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
3537987 |
+ |
SDK1 |
NNNTCACCTAGTTTGCATGT |
NGG |
2 |
0.44 |
Tier II |
| 3 |
NC_000018.10 |
18 |
59692497 |
- |
CCBE1 |
NNNTCACCAAGTTCCCATGG |
NGG |
2 |
0.0481 |
Tier II |
| 4 |
NC_000007.14 |
7 |
93310641 |
- |
VPS50 |
NNNTCACCACGTTCGCATGT |
NGA |
2 |
0.0231 |
Tier II |
| 5 |
NC_000004.12 |
4 |
168278638 |
- |
DDX60 |
NNNTCACCATGTTGGCATGT |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000011.10 |
11 |
77802752 |
+ |
RSF1 |
NNNTCACCATGTTGGCATGT |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000012.12 |
12 |
91617255 |
- |
LOC105369896 |
NNNTCACCATGTTGGCATGT |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
53498882 |
- |
Atm |
NNNTCATCAAGTTCGGATGT |
NGG |
2 |
0.125 |
Tier I |
| 2 |
NC_000080.6 |
14 |
23984682 |
+ |
Kcnma1 |
NNNCCACCATGTTCGCATGT |
NGG |
2 |
0.5709 |
Tier II |
| 3 |
NC_000072.6 |
6 |
127972423 |
- |
Tspan9 |
NNNTTATCAAGTTCGCATGT |
NGG |
2 |
0.517 |
Tier II |
| 4 |
NC_000074.6 |
8 |
106938073 |
- |
Sntb2 |
NNNTCACCAGGTACGCATGT |
NGG |
2 |
0.3846 |
Tier II |
| 5 |
NC_000069.6 |
3 |
155172729 |
+ |
Lrriq3 |
NNNTCCCCAAGTTCCCATGT |
NGG |
2 |
0.124 |
Tier II |
| 6 |
NC_000069.6 |
3 |
57518296 |
- |
Wwtr1 |
NNNTCACCAACTTCCCATGT |
NGG |
2 |
0.1169 |
Tier II |
| 7 |
NC_000078.6 |
12 |
105363588 |
- |
Tunar |
NNNTCACCAAATCCGCATGT |
NGG |
2 |
0.7895 |
Tier III |
| 8 |
NC_000074.6 |
8 |
122836671 |
+ |
Gm35466 |
NNNTCACCAAGTTCGTACGT |
NGG |
2 |
0.1319 |
Tier III |
Other clones with same target sequence:
(none)
