Construct: sgRNA BRDN0001149519
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTGCTTCCAGACGACAAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCZ (5590)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76632
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
2156088 |
+ |
PRKCZ |
NNNGCTTCCAGACGACAAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
50482627 |
- |
ADM2 |
NNNGCTTCCAGACCACAGGT |
NGG |
2 |
0.1714 |
Tier I |
| 3 |
NC_000010.11 |
10 |
73817410 |
- |
CAMK2G |
NNNGCTTCCAGACAACAATT |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000010.11 |
10 |
20059863 |
- |
PLXDC2 |
NNNGCTTCTAGACCACAAGT |
NGG |
2 |
0.375 |
Tier II |
| 5 |
NC_000010.11 |
10 |
113903292 |
+ |
NHLRC2 |
NNNGCTTCCAGAAGACAAGA |
NGG |
2 |
0.2163 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
18948574 |
+ |
Xkr5 |
NNNGCTTCCAGACGCCAAGT |
NGC |
2 |
0.0061 |
Tier I |
| 2 |
NC_000070.6 |
4 |
119467312 |
+ |
Rimkla |
NNNGCTTCCAGATGACAAGT |
NGG |
1 |
0.7 |
Tier II |
| 3 |
NC_000075.6 |
9 |
29950642 |
- |
Ntm |
NNNACTTCCAGACCACAAGT |
NGG |
2 |
0.3857 |
Tier II |
| 4 |
NC_000077.6 |
11 |
44974784 |
- |
Ebf1 |
NNNGCTTCCACACGACAAAT |
NGG |
2 |
0.3061 |
Tier II |
| 5 |
NC_000076.6 |
10 |
18410352 |
+ |
Nhsl1 |
NNNGCTTACAGAAGACAAGT |
NGG |
2 |
0.25 |
Tier II |
| 6 |
NC_000078.6 |
12 |
81950767 |
+ |
Pcnx |
NNNGCTTCCAGTCAACAAGT |
NGG |
2 |
0.25 |
Tier II |
| 7 |
NC_000068.7 |
2 |
26373573 |
+ |
Snapc4 |
NNNGCTTCCAGAAGACAGGT |
NGG |
2 |
0.1538 |
Tier II |
Other clones with same target sequence:
(none)
