Construct: sgRNA BRDN0001149521

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTCTAGGGGGAAATTTGCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK17A (9263)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77917

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 43595912 + STK17A NNNTAGGGGGAAATTTGCAG NGG 0 1.0 Tier I
2 NC_000022.11 22 26292912 - SEZ6L NNNAAGGGGGAGATTTGCAG NGG 2 0.3439 Tier I
3 NC_000007.14 7 152358653 - KMT2C NNNTAGGGGGAAAACTGCAG NGG 2 0.1688 Tier I
4 NC_000005.10 5 154362466 + GALNT10 NNNTAGGGGGAAAATTACAG NGG 2 0.5778 Tier II
5 NC_000012.12 12 99666536 + ANKS1B NNNTAGGGGTAAATTTGTAG NGG 2 0.5223 Tier II
6 NC_000002.12 2 239930351 - NDUFA10 NNNAAGGAGGAAATTTGCAG NGG 2 0.4762 Tier II
7 NC_000003.12 3 183154522 + LAMP3 NNNTTGGGGGAAATTTGCAA NGG 2 0.3409 Tier II
8 NC_000017.11 17 33024765 - ASIC2 NNNTAGGGGGAAATCTGCAA NGG 2 0.2557 Tier II
9 NC_000001.11 1 206453605 - SRGAP2 NNNTAGGGGGTGATTTGCAG NGG 2 0.2222 Tier II
10 NC_000011.10 11 126999927 - KIRREL3 NNNTGGGGGGTAATTTGCAG NGG 2 0.2215 Tier II
11 NC_000009.12 9 120842101 - PSMD5 NNNTAGGGGGAAACCTGCAG NGG 2 0.0779 Tier II
12 NC_000005.10 5 79696630 - CMYA5 NNNTAGGAGGAAATTTGCAG NGA 2 0.0694 Tier II
13 NC_000014.9 14 59254132 + DAAM1 NNNTAGGGGGACATTTTCAG NGG 2 0.0658 Tier II
14 NC_000021.9 21 25955084 - APP NNNTAGGGGGAAATTTGCAA NGA 2 0.0651 Tier II
15 NC_000005.10 5 73865495 + ARHGEF28 NNNCAGGGGGAAATTTGCAG NTG 2 0.0252 Tier II
16 NC_000001.11 1 222535704 - HHIPL2 NNNTAGGGGGAAATTTGAAG NTG 2 0.021 Tier II
17 NC_000010.11 10 98896318 + HPSE2 NNNTAGGGGGAAATTTGGAG NGA 2 0.0093 Tier II
18 NC_000005.10 5 103668712 - LOC105379107 NNNTAAAGGGAAATTTGCAG NGG 2 1.0 Tier III
19 NC_000006.12 6 87936475 + LOC105377882 NNNGAGGGAGAAATTTGCAG NGG 2 0.4018 Tier III
20 NC_000015.10 15 97421017 + LINC02254 NNNTAGGGGGCAATTTGTAG NGG 2 0.2571 Tier III
21 NC_000015.10 15 97421017 + LINC02253 NNNTAGGGGGCAATTTGTAG NGG 2 0.2571 Tier III
22 NC_000011.10 11 64419794 - LOC105369341 NNNTAGGGGGAGATTTTCAG NGG 2 0.1806 Tier III
23 NC_000011.10 11 64419794 - LOC124902689 NNNTAGGGGGAGATTTTCAG NGG 2 0.1806 Tier III
24 NC_000008.11 8 48574649 + LOC101929268 NNNTAGGGAGACATTTGCAG NGG 2 0.1692 Tier III
25 NC_000005.10 5 136023478 + LOC105379189 NNNAAGGGGGAATTTTGCAG NGG 2 0.1429 Tier III
26 NC_000001.11 1 193662012 - LOC124904475 NNNTAGGGGAAAATTTGCAG NTG 2 0.0364 Tier III
27 NC_000005.10 5 163306022 - LOC105377700 NNNTAGGGGGAAATTTGCAG NGC 1 0.0222 Tier III
28 NC_000004.12 4 77402519 + LOC105377295 NNNTAGGGGGAACTTTGCAG NGA 2 0.0146 Tier III
29 NC_000010.11 10 7018446 + LOC105376387 NNNTAGGGGGAAATCTGCAG NTG 2 0.0106 Tier III
30 NC_000012.12 12 18758395 + LOC102724227 NNNTAGGGGGAAAGTTGCAG NGG 1 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 104791645 - Clmn NNNTAGGGGAAGATTTGCAG NGG 2 0.6741 Tier II
2 NC_000083.6 17 81519527 + Slc8a1 NNNTAGAGGGAAGTTTGCAG NGG 2 0.6522 Tier II
3 NC_000086.7 X 79445995 + Cfap47 NNNTAGGGGAAAATATGCAG NGG 2 0.5404 Tier II
4 NC_000073.6 7 37517929 - Zfp536 NNNTAGGGGGAAATTAGAAG NGG 2 0.4895 Tier II
5 NC_000070.6 4 36315886 + Lingo2 NNNGAGGGGGAATTTTGCAG NGG 2 0.1875 Tier II
6 NC_000068.7 2 130814600 - 4930402H24Rik NNNTAGGGGGAGCTTTGCAG NGG 2 0.152 Tier II
7 NC_000077.6 11 9544831 + Abca13 NNNTAGGGGGAAATATGCAG NAG 2 0.1501 Tier II
8 NC_000077.6 11 67045720 + Adprm NNNTAGGGGGAAATTAGCAG NGA 2 0.0631 Tier II
9 NC_000085.6 19 28872138 - Slc1a1 NNNTAGGAGGAAATGTGCAG NGG 2 0.05 Tier II
10 NC_000081.6 15 25409722 - Basp1 NNNTGGGGGGAAATTTGCAG NGA 2 0.05 Tier II
11 NC_000068.7 2 145195953 + Slc24a3 NNNTATGGGGAAATGTGCAG NGG 2 0.0333 Tier II
12 NC_000076.6 10 32190675 - Nkain2 NNNTAGGGGGAAAGTTGCAT NGG 2 0.0 Tier II
13 NC_000084.6 18 64243465 - St8sia3os NNNTAGAGAGAAATTTGCAG NGG 2 0.6429 Tier III
14 NC_000067.6 1 73300743 + Gm29185 NNNGAGGGGGAAGTTTGCAG NGG 2 0.4076 Tier III
15 NC_000070.6 4 14227485 - Gm2560 NNNTAGGGGGAAATATGCAG NAG 2 0.1501 Tier III
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Other clones with same target sequence:

(none)