Construct: sgRNA BRDN0001149521
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTCTAGGGGGAAATTTGCAG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- STK17A (9263)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000007.14 | 7 | 43595912 | + | STK17A | NNNTAGGGGGAAATTTGCAG | NGG | 0 | 1.0 | Tier I |
2 | NC_000022.11 | 22 | 26292912 | - | SEZ6L | NNNAAGGGGGAGATTTGCAG | NGG | 2 | 0.3439 | Tier I |
3 | NC_000007.14 | 7 | 152358653 | - | KMT2C | NNNTAGGGGGAAAACTGCAG | NGG | 2 | 0.1688 | Tier I |
4 | NC_000005.10 | 5 | 154362466 | + | GALNT10 | NNNTAGGGGGAAAATTACAG | NGG | 2 | 0.5778 | Tier II |
5 | NC_000012.12 | 12 | 99666536 | + | ANKS1B | NNNTAGGGGTAAATTTGTAG | NGG | 2 | 0.5223 | Tier II |
6 | NC_000002.12 | 2 | 239930351 | - | NDUFA10 | NNNAAGGAGGAAATTTGCAG | NGG | 2 | 0.4762 | Tier II |
7 | NC_000003.12 | 3 | 183154522 | + | LAMP3 | NNNTTGGGGGAAATTTGCAA | NGG | 2 | 0.3409 | Tier II |
8 | NC_000017.11 | 17 | 33024765 | - | ASIC2 | NNNTAGGGGGAAATCTGCAA | NGG | 2 | 0.2557 | Tier II |
9 | NC_000001.11 | 1 | 206453605 | - | SRGAP2 | NNNTAGGGGGTGATTTGCAG | NGG | 2 | 0.2222 | Tier II |
10 | NC_000011.10 | 11 | 126999927 | - | KIRREL3 | NNNTGGGGGGTAATTTGCAG | NGG | 2 | 0.2215 | Tier II |
11 | NC_000009.12 | 9 | 120842101 | - | PSMD5 | NNNTAGGGGGAAACCTGCAG | NGG | 2 | 0.0779 | Tier II |
12 | NC_000005.10 | 5 | 79696630 | - | CMYA5 | NNNTAGGAGGAAATTTGCAG | NGA | 2 | 0.0694 | Tier II |
13 | NC_000014.9 | 14 | 59254132 | + | DAAM1 | NNNTAGGGGGACATTTTCAG | NGG | 2 | 0.0658 | Tier II |
14 | NC_000021.9 | 21 | 25955084 | - | APP | NNNTAGGGGGAAATTTGCAA | NGA | 2 | 0.0651 | Tier II |
15 | NC_000005.10 | 5 | 73865495 | + | ARHGEF28 | NNNCAGGGGGAAATTTGCAG | NTG | 2 | 0.0252 | Tier II |
16 | NC_000001.11 | 1 | 222535704 | - | HHIPL2 | NNNTAGGGGGAAATTTGAAG | NTG | 2 | 0.021 | Tier II |
17 | NC_000010.11 | 10 | 98896318 | + | HPSE2 | NNNTAGGGGGAAATTTGGAG | NGA | 2 | 0.0093 | Tier II |
18 | NC_000005.10 | 5 | 103668712 | - | LOC105379107 | NNNTAAAGGGAAATTTGCAG | NGG | 2 | 1.0 | Tier III |
19 | NC_000006.12 | 6 | 87936475 | + | LOC105377882 | NNNGAGGGAGAAATTTGCAG | NGG | 2 | 0.4018 | Tier III |
20 | NC_000015.10 | 15 | 97421017 | + | LINC02254 | NNNTAGGGGGCAATTTGTAG | NGG | 2 | 0.2571 | Tier III |
21 | NC_000015.10 | 15 | 97421017 | + | LINC02253 | NNNTAGGGGGCAATTTGTAG | NGG | 2 | 0.2571 | Tier III |
22 | NC_000011.10 | 11 | 64419794 | - | LOC105369341 | NNNTAGGGGGAGATTTTCAG | NGG | 2 | 0.1806 | Tier III |
23 | NC_000011.10 | 11 | 64419794 | - | LOC124902689 | NNNTAGGGGGAGATTTTCAG | NGG | 2 | 0.1806 | Tier III |
24 | NC_000008.11 | 8 | 48574649 | + | LOC101929268 | NNNTAGGGAGACATTTGCAG | NGG | 2 | 0.1692 | Tier III |
25 | NC_000005.10 | 5 | 136023478 | + | LOC105379189 | NNNAAGGGGGAATTTTGCAG | NGG | 2 | 0.1429 | Tier III |
26 | NC_000001.11 | 1 | 193662012 | - | LOC124904475 | NNNTAGGGGAAAATTTGCAG | NTG | 2 | 0.0364 | Tier III |
27 | NC_000005.10 | 5 | 163306022 | - | LOC105377700 | NNNTAGGGGGAAATTTGCAG | NGC | 1 | 0.0222 | Tier III |
28 | NC_000004.12 | 4 | 77402519 | + | LOC105377295 | NNNTAGGGGGAACTTTGCAG | NGA | 2 | 0.0146 | Tier III |
29 | NC_000010.11 | 10 | 7018446 | + | LOC105376387 | NNNTAGGGGGAAATCTGCAG | NTG | 2 | 0.0106 | Tier III |
30 | NC_000012.12 | 12 | 18758395 | + | LOC102724227 | NNNTAGGGGGAAAGTTGCAG | NGG | 1 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000078.6 | 12 | 104791645 | - | Clmn | NNNTAGGGGAAGATTTGCAG | NGG | 2 | 0.6741 | Tier II |
2 | NC_000083.6 | 17 | 81519527 | + | Slc8a1 | NNNTAGAGGGAAGTTTGCAG | NGG | 2 | 0.6522 | Tier II |
3 | NC_000086.7 | X | 79445995 | + | Cfap47 | NNNTAGGGGAAAATATGCAG | NGG | 2 | 0.5404 | Tier II |
4 | NC_000073.6 | 7 | 37517929 | - | Zfp536 | NNNTAGGGGGAAATTAGAAG | NGG | 2 | 0.4895 | Tier II |
5 | NC_000070.6 | 4 | 36315886 | + | Lingo2 | NNNGAGGGGGAATTTTGCAG | NGG | 2 | 0.1875 | Tier II |
6 | NC_000068.7 | 2 | 130814600 | - | 4930402H24Rik | NNNTAGGGGGAGCTTTGCAG | NGG | 2 | 0.152 | Tier II |
7 | NC_000077.6 | 11 | 9544831 | + | Abca13 | NNNTAGGGGGAAATATGCAG | NAG | 2 | 0.1501 | Tier II |
8 | NC_000077.6 | 11 | 67045720 | + | Adprm | NNNTAGGGGGAAATTAGCAG | NGA | 2 | 0.0631 | Tier II |
9 | NC_000085.6 | 19 | 28872138 | - | Slc1a1 | NNNTAGGAGGAAATGTGCAG | NGG | 2 | 0.05 | Tier II |
10 | NC_000081.6 | 15 | 25409722 | - | Basp1 | NNNTGGGGGGAAATTTGCAG | NGA | 2 | 0.05 | Tier II |
11 | NC_000068.7 | 2 | 145195953 | + | Slc24a3 | NNNTATGGGGAAATGTGCAG | NGG | 2 | 0.0333 | Tier II |
12 | NC_000076.6 | 10 | 32190675 | - | Nkain2 | NNNTAGGGGGAAAGTTGCAT | NGG | 2 | 0.0 | Tier II |
13 | NC_000084.6 | 18 | 64243465 | - | St8sia3os | NNNTAGAGAGAAATTTGCAG | NGG | 2 | 0.6429 | Tier III |
14 | NC_000067.6 | 1 | 73300743 | + | Gm29185 | NNNGAGGGGGAAGTTTGCAG | NGG | 2 | 0.4076 | Tier III |
15 | NC_000070.6 | 4 | 14227485 | - | Gm2560 | NNNTAGGGGGAAATATGCAG | NAG | 2 | 0.1501 | Tier III |