Construct: sgRNA BRDN0001149526
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTGATACGTCCCTGCAGGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKAG1 (5571)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77746
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
49005744 |
- |
PRKAG1 |
NNNGATACGTCCCTGCAGGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
1667055 |
- |
PXDN |
NNNGCCACGTCCCTGCAGGT |
NGG |
2 |
0.4545 |
Tier II |
| 3 |
NC_000005.10 |
5 |
138333638 |
+ |
CDC25C |
NNNTATACTTCCCTGCAGGT |
NGG |
2 |
0.1939 |
Tier II |
| 4 |
NC_000001.11 |
1 |
33870424 |
+ |
CSMD2 |
NNNGATACGACCCTTCAGGT |
NGG |
2 |
0.1224 |
Tier II |
| 5 |
NC_000021.9 |
21 |
44919272 |
- |
ITGB2 |
NNNGCTACGTCCCTGCAGGG |
NGG |
2 |
0.0882 |
Tier II |
| 6 |
NC_000012.12 |
12 |
49005744 |
- |
DDN-AS1 |
NNNGATACGTCCCTGCAGGT |
NGG |
0 |
1.0 |
Tier III |
| 7 |
NC_000002.12 |
2 |
867445 |
- |
LINC01115 |
NNNGAGAGGTCCCTGCAGGT |
NGG |
2 |
0.3673 |
Tier III |
| 8 |
NC_000005.10 |
5 |
138333638 |
+ |
LOC100128966 |
NNNTATACTTCCCTGCAGGT |
NGG |
2 |
0.1939 |
Tier III |
| 9 |
NC_000001.11 |
1 |
33870424 |
+ |
CSMD2-AS1 |
NNNGATACGACCCTTCAGGT |
NGG |
2 |
0.1224 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
142428902 |
- |
Foxk1 |
NNNGACACGTCCCAGCAGGT |
NGG |
2 |
0.5628 |
Tier II |
| 2 |
NC_000068.7 |
2 |
177363788 |
- |
Gm14412 |
NNNGATAGGTCCCTTCAGGT |
NGG |
2 |
0.0918 |
Tier III |
Other clones with same target sequence:
(none)
