Construct: sgRNA BRDN0001149528
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTTAAGGAATACTGCCCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP4K2A (5305)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76575
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
22607983 |
- |
PIP4K2A |
NNNTAAGGAATACTGCCCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
53377237 |
+ |
TSHZ2 |
NNNCAAGGAATACTGCCTGA |
NGG |
2 |
0.416 |
Tier II |
| 3 |
NC_000016.10 |
16 |
10760118 |
- |
NUBP1 |
NNNTAAGGAATACTGCCCAA |
NGT |
2 |
0.0115 |
Tier II |
| 4 |
NC_000016.10 |
16 |
10760118 |
- |
TVP23A |
NNNTAAGGAATACTGCCCAA |
NGT |
2 |
0.0115 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
18906285 |
- |
Pip4k2a |
NNNTAAGGAATACTGCCCAA |
NGG |
1 |
0.7143 |
Tier I |
| 2 |
NC_000070.6 |
4 |
127118200 |
+ |
Zmym6 |
NNNTATAGAATACTGCCCGA |
NGG |
2 |
0.7143 |
Tier II |
| 3 |
NC_000071.6 |
5 |
128273783 |
- |
Tmem132d |
NNNTAAGGAATATTGCCCAA |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000067.6 |
1 |
181217745 |
- |
Wdr26 |
NNNTAAGGAAGACTGCCCAA |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000067.6 |
1 |
181217745 |
- |
Gm38664 |
NNNTAAGGAAGACTGCCCAA |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000079.6 |
13 |
53243153 |
+ |
Ror2 |
NNNCAAGGAAGACTGCCCGA |
NGG |
2 |
0.2588 |
Tier II |
| 7 |
NC_000075.6 |
9 |
104024024 |
- |
Nphp3 |
NNNTAAGGAATACTGCCTGA |
NAG |
2 |
0.1667 |
Tier II |
| 8 |
NC_000072.6 |
6 |
39678845 |
- |
Braf |
NNNTAAGGAATACTGCCAGA |
NAG |
2 |
0.1396 |
Tier II |
| 9 |
NC_000080.6 |
14 |
22769368 |
- |
Lrmda |
NNNTAAGGAATACTTCCTGA |
NGG |
2 |
0.0918 |
Tier II |
| 10 |
NC_000078.6 |
12 |
56266497 |
+ |
Gm46351 |
NNNGAAGGAATACTGCCCAA |
NGG |
2 |
0.4464 |
Tier III |
| 11 |
NC_000080.6 |
14 |
22769368 |
- |
Gm30926 |
NNNTAAGGAATACTTCCTGA |
NGG |
2 |
0.0918 |
Tier III |
Other clones with same target sequence:
(none)
