Construct: sgRNA BRDN0001149529
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAAGTCAGAGAGCTCGTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SH3BP4 (23677)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78087
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
235042515 |
- |
SH3BP4 |
NNNAGTCAGAGAGCTCGTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
102613453 |
+ |
NFKB1 |
NNNAGTCAGAGAGCTGGTGG |
NGG |
2 |
0.0385 |
Tier I |
| 3 |
NC_000011.10 |
11 |
13313022 |
+ |
BMAL1 |
NNNAGACAGTGAGCTCGTTG |
NGG |
2 |
0.7647 |
Tier II |
| 4 |
NC_000002.12 |
2 |
29250349 |
+ |
ALK |
NNNAGTCATAGAGCTCGTTT |
NGG |
2 |
0.3733 |
Tier II |
| 5 |
NC_000022.11 |
22 |
29335096 |
+ |
AP1B1 |
NNNACTCAGAGAGCTCGCTG |
NGG |
2 |
0.3367 |
Tier II |
| 6 |
NC_000011.10 |
11 |
119153542 |
- |
ABCG4 |
NNNAGTCAGTGAGCTCCTTG |
NGG |
2 |
0.2076 |
Tier II |
| 7 |
NC_000022.11 |
22 |
36285036 |
+ |
MYH9 |
NNNAGACAGAGAGCTGGTTG |
NGG |
2 |
0.1333 |
Tier II |
| 8 |
NC_000012.12 |
12 |
31008347 |
+ |
TSPAN11 |
NNNAGTCACAGAGCTCCTTG |
NGG |
2 |
0.1267 |
Tier II |
| 9 |
NC_000014.9 |
14 |
31584904 |
+ |
NUBPL |
NNNAGTGAGAGAGCTCTTTG |
NGG |
2 |
0.1176 |
Tier II |
| 10 |
NC_000012.12 |
12 |
80994 |
+ |
IQSEC3 |
NNNAGTCAGAGAGATTGTTG |
NGG |
2 |
0.1077 |
Tier II |
| 11 |
NC_000023.11 |
X |
132064022 |
- |
STK26 |
NNNAGTCAGAGAGCTCTCTG |
NGG |
2 |
0.1071 |
Tier II |
| 12 |
NC_000020.11 |
20 |
17643229 |
- |
RRBP1 |
NNNAGTCAGAGAGCTCTTCG |
NGG |
2 |
0.069 |
Tier II |
| 13 |
NC_000002.12 |
2 |
216179740 |
+ |
XRCC5 |
NNNAGTCAGAGAGCTGGCTG |
NGG |
2 |
0.0659 |
Tier II |
| 14 |
NC_000005.10 |
5 |
20016626 |
+ |
CDH18 |
NNNAGTCAGAGTGCTGGTTG |
NGG |
2 |
0.0513 |
Tier II |
| 15 |
NC_000001.11 |
1 |
210025089 |
+ |
SYT14 |
NNNAGTCAGAGAGGTAGTTG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000010.11 |
10 |
114757885 |
- |
ABLIM1 |
NNNAGTCAGAGAGGTAGTTG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000003.12 |
3 |
113777937 |
+ |
ATP6V1A |
NNNAGTCAGAGAGGTCATTG |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000003.12 |
3 |
169868149 |
- |
LRRC31 |
NNNAGTCAGAGAGGTCGCTG |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000003.12 |
3 |
31811837 |
+ |
OSBPL10 |
NNNAGTCAGAGAGGTCTTTG |
NGG |
2 |
0.0 |
Tier II |
| 20 |
NC_000004.12 |
4 |
102613453 |
+ |
LOC105377347 |
NNNAGTCAGAGAGCTGGTGG |
NGG |
2 |
0.0385 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
75170837 |
+ |
Pdgfra |
NNNAGTCAGAGAGTTCGTGG |
NGG |
2 |
0.1833 |
Tier I |
| 2 |
NC_000076.6 |
10 |
76257151 |
- |
S100b |
NNNAGTGAGAGAGCTCGTTG |
NTG |
2 |
0.0183 |
Tier I |
| 3 |
NC_000074.6 |
8 |
47502062 |
- |
Trappc11 |
NNNAGTCAGAGAACTCATTG |
NGG |
2 |
0.8615 |
Tier II |
| 4 |
NC_000082.6 |
16 |
34428741 |
+ |
Kalrn |
NNNAGTCAGAGAGCTAGATG |
NGG |
2 |
0.6667 |
Tier II |
| 5 |
NC_000075.6 |
9 |
22628397 |
+ |
Bbs9 |
NNNAATCAGAGAGCTCCTTG |
NGG |
2 |
0.2039 |
Tier II |
| 6 |
NC_000067.6 |
1 |
66687387 |
+ |
Unc80 |
NNNAGTCAGAGAGCTCGTCT |
NGG |
2 |
0.1931 |
Tier II |
| 7 |
NC_000071.6 |
5 |
91559927 |
+ |
Parm1 |
NNNATGCAGAGAGCTCGTTG |
NGG |
2 |
0.1714 |
Tier II |
| 8 |
NC_000078.6 |
12 |
116844840 |
+ |
Ptprn2 |
NNNAGGCAGAGAGCTCCTTG |
NGG |
2 |
0.1345 |
Tier II |
| 9 |
NC_000071.6 |
5 |
105704311 |
+ |
Lrrc8d |
NNNAGTCAGAGAGCTTGCTG |
NGG |
2 |
0.1319 |
Tier II |
| 10 |
NC_000071.6 |
5 |
105704311 |
+ |
Gm42166 |
NNNAGTCAGAGAGCTTGCTG |
NGG |
2 |
0.1319 |
Tier II |
| 11 |
NC_000076.6 |
10 |
118153026 |
- |
Mdm1 |
NNNAGTCAGAGAGCCCCTTG |
NGG |
2 |
0.0642 |
Tier II |
| 12 |
NC_000077.6 |
11 |
117332792 |
- |
Septin9 |
NNNAGTCAGAGAGCCCCTTG |
NGG |
2 |
0.0642 |
Tier II |
| 13 |
NC_000067.6 |
1 |
89872417 |
+ |
Agap1 |
NNNAGTCAGAGAGCACGTTG |
NTG |
2 |
0.0226 |
Tier II |
| 14 |
NC_000077.6 |
11 |
14073211 |
+ |
4930554G24Rik |
NNNAGTCAGAGAACTTGTTG |
NGG |
2 |
0.284 |
Tier III |
| 15 |
NC_000077.6 |
11 |
117332792 |
- |
Gm51932 |
NNNAGTCAGAGAGCCCCTTG |
NGG |
2 |
0.0642 |
Tier III |
Other clones with same target sequence:
(none)
