Construct: sgRNA BRDN0001149531

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGCTGCGGAAGGACCCGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK2A (815)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76928

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 150252014 - CAMK2A NNNCTGCGGAAGGACCCGTA NGG 0 1.0 Tier I
2 NC_000001.11 1 236007704 - NID1 NNNCTGCTGAAGGACCCGCA NGG 2 0.1724 Tier II
3 NC_000007.14 7 5361264 - TNRC18 NNNCTGCGGAAGGACCCGCT NGG 2 0.1655 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 60957156 + Camk2a NNNCTGAGGAAGGACCCGTA NGG 1 0.75 Tier I
2 NC_000067.6 1 55715224 + Plcl1 NNNCTGGGGAAGGACACGTA NGG 2 0.4706 Tier II
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Other clones with same target sequence:

(none)