Construct: sgRNA BRDN0001149536

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCTTCCTTATCTCCTACGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIP4K2C (79837)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77683

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 57595954 + PIP4K2C NNNTCCTTATCTCCTACGAT NGG 0 1.0 Tier I
2 NC_000005.10 5 5245754 + ADAMTS16 NNNTCCTTATCTCTTACAAT NGG 2 0.5077 Tier II
3 NC_000001.11 1 52158672 - ZFYVE9 NNNTCCTTATCACCTACGAA NGG 2 0.45 Tier II
4 NC_000001.11 1 52158672 - LOC107984956 NNNTCCTTATCACCTACGAA NGG 2 0.45 Tier II
5 NC_000003.12 3 36869331 - TRANK1 NNNTCCTTATCTCTTAGGAT NGG 2 0.0431 Tier II
6 NC_000008.11 8 120409852 + MRPL13 NNNTCCTTATCTCCTACCAT NGC 2 0.0106 Tier II
7 NC_000002.12 2 30234696 + LBH NNNTCCTTATCTCCTAAGAT NGT 2 0.0075 Tier II
8 NC_000014.9 14 48234023 + LOC105370482 NNNTAGTTATCTCCTACGAT NGG 2 0.2857 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 96197640 - Gm38469 NNNTCCTTCTCTCTTACGAT NGG 2 0.419 Tier II
2 NC_000071.6 5 143468091 - Daglb NNNTTCTTATCTCCTACGAT NTG 2 0.0248 Tier II
3 NC_000086.7 X 51645013 + Hs6st2 NNNTCCTTATCTCCTACCAT NTG 2 0.0186 Tier II
4 NC_000081.6 15 96197640 - 2610037D02Rik NNNTCCTTCTCTCTTACGAT NGG 2 0.419 Tier III
5 NC_000081.6 15 96197640 - Gm4371 NNNTCCTTCTCTCTTACGAT NGG 2 0.419 Tier III
6 NC_000079.6 13 51362399 - Gm46414 NNNTCCTCATCTCCTACGCT NGG 2 0.2069 Tier III
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Other clones with same target sequence:

(none)