Construct: sgRNA BRDN0001149536
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTTCCTTATCTCCTACGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP4K2C (79837)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77683
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
57595954 |
+ |
PIP4K2C |
NNNTCCTTATCTCCTACGAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
5245754 |
+ |
ADAMTS16 |
NNNTCCTTATCTCTTACAAT |
NGG |
2 |
0.5077 |
Tier II |
| 3 |
NC_000001.11 |
1 |
52158672 |
- |
ZFYVE9 |
NNNTCCTTATCACCTACGAA |
NGG |
2 |
0.45 |
Tier II |
| 4 |
NC_000001.11 |
1 |
52158672 |
- |
LOC107984956 |
NNNTCCTTATCACCTACGAA |
NGG |
2 |
0.45 |
Tier II |
| 5 |
NC_000003.12 |
3 |
36869331 |
- |
TRANK1 |
NNNTCCTTATCTCTTAGGAT |
NGG |
2 |
0.0431 |
Tier II |
| 6 |
NC_000008.11 |
8 |
120409852 |
+ |
MRPL13 |
NNNTCCTTATCTCCTACCAT |
NGC |
2 |
0.0106 |
Tier II |
| 7 |
NC_000002.12 |
2 |
30234696 |
+ |
LBH |
NNNTCCTTATCTCCTAAGAT |
NGT |
2 |
0.0075 |
Tier II |
| 8 |
NC_000014.9 |
14 |
48234023 |
+ |
LOC105370482 |
NNNTAGTTATCTCCTACGAT |
NGG |
2 |
0.2857 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
96197640 |
- |
Gm38469 |
NNNTCCTTCTCTCTTACGAT |
NGG |
2 |
0.419 |
Tier II |
| 2 |
NC_000071.6 |
5 |
143468091 |
- |
Daglb |
NNNTTCTTATCTCCTACGAT |
NTG |
2 |
0.0248 |
Tier II |
| 3 |
NC_000086.7 |
X |
51645013 |
+ |
Hs6st2 |
NNNTCCTTATCTCCTACCAT |
NTG |
2 |
0.0186 |
Tier II |
| 4 |
NC_000081.6 |
15 |
96197640 |
- |
2610037D02Rik |
NNNTCCTTCTCTCTTACGAT |
NGG |
2 |
0.419 |
Tier III |
| 5 |
NC_000081.6 |
15 |
96197640 |
- |
Gm4371 |
NNNTCCTTCTCTCTTACGAT |
NGG |
2 |
0.419 |
Tier III |
| 6 |
NC_000079.6 |
13 |
51362399 |
- |
Gm46414 |
NNNTCCTCATCTCCTACGCT |
NGG |
2 |
0.2069 |
Tier III |
Other clones with same target sequence:
(none)
