Construct: sgRNA BRDN0001149537
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGTTGCTAGTAGAACAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PKN2 (5586)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77927
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
88760263 |
- |
PKN2 |
NNNTTGCTAGTAGAACAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
16513243 |
+ |
LOC101929028 |
NNNATGCCAGTAGAACAACG |
NGG |
2 |
0.4762 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
142839322 |
+ |
Pkn2 |
NNNTTGCTAGTAGAACTACG |
NGA |
2 |
0.0093 |
Tier I |
| 2 |
NC_000086.7 |
X |
83814353 |
+ |
Dmd |
NNNTTGGTAGTAGAACAACA |
NGG |
2 |
0.4412 |
Tier II |
| 3 |
NC_000072.6 |
6 |
47101526 |
- |
Cntnap2 |
NNNTAGCTATTAGAACAACG |
NGG |
2 |
0.4063 |
Tier II |
| 4 |
NC_000081.6 |
15 |
44581650 |
- |
Pkhd1l1 |
NNNTTTCTAGTAGAACAAAG |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000082.6 |
16 |
21452824 |
+ |
Vps8 |
NNNTTGCTAGTAGAACAAGT |
NGG |
2 |
0.0875 |
Tier II |
Other clones with same target sequence:
(none)
