Construct: sgRNA BRDN0001149539

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAAATACCTATTGAAAACCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3C2G (5288)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75741

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 18325094 + PIK3C2G NNNATACCTATTGAAAACCC NGG 0 1.0 Tier I
2 NC_000007.14 7 74999470 + CASTOR2 NNNAGACCCATTGAAAACCC NGG 2 0.5908 Tier II
3 NC_000005.10 5 155999195 - SGCD NNNATATCTACTGAAAACCC NGG 2 0.5417 Tier II
4 NC_000012.12 12 39962681 - SLC2A13 NNNATATCTATTGAAAAACC NGG 2 0.4375 Tier II
5 NC_000019.10 19 45532159 + OPA3 NNNATCCCTATTAAAAACCC NGG 2 0.4196 Tier II
6 NC_000003.12 3 128243595 + EEFSEC NNNATACAGATTGAAAACCC NGG 2 0.4024 Tier II
7 NC_000019.10 19 31221281 - TSHZ3 NNNATATCTATTGGAAACCC NGG 2 0.3792 Tier II
8 NC_000005.10 5 94411185 + KIAA0825 NNNATACATATTGTAAACCC NGG 2 0.3467 Tier II
9 NC_000017.11 17 55125465 + STXBP4 NNNAAACCTATTGTAAACCC NGG 2 0.2667 Tier II
10 NC_000002.12 2 227260382 + COL4A3 NNNCTACATATTGAAAACCC NGG 2 0.2294 Tier II
11 NC_000001.11 1 34909473 - DLGAP3 NNNATACCTATTGAAATCCT NGG 2 0.04 Tier II
12 NC_000017.11 17 30135036 - NSRP1 NNNATACCCATTGAAAACCC NTG 2 0.036 Tier II
13 NC_000001.11 1 202426338 - PPP1R12B NNNATACCTATTAAAAACCC NGC 2 0.0205 Tier II
14 NC_000016.10 16 53172550 - CHD9 NNNATACCTATTAAAAACCC NGC 2 0.0205 Tier II
15 NC_000018.10 18 9344732 + TWSG1 NNNATACCTATTGAAAACCA NTG 2 0.0195 Tier II
16 NC_000005.10 5 176393490 + CLTB NNNATACCTATGGAAAACCC NGC 2 0.0111 Tier II
17 NC_000005.10 5 176393490 + ARL10 NNNATACCTATGGAAAACCC NGC 2 0.0111 Tier II
18 NC_000005.10 5 148129335 + SPINK5 NNNATACCTATTGAAAACAC NGC 2 0.0095 Tier II
19 NC_000004.12 4 84886691 - WDFY3 NNNATACCTATTGAAAAACC NGT 2 0.0087 Tier II
20 NC_000012.12 12 89496772 - POC1B NNNATCCCTATTGAAAACCC NGT 2 0.0073 Tier II
21 NC_000011.10 11 12842304 + TEAD1 NNNATACCTATTGAAAACCC NTA 2 0.0 Tier II
22 NC_000007.14 7 100242193 - CASTOR3P NNNAGACCCATTGAAAACCC NGG 2 0.5908 Tier III
23 NC_000017.11 17 73786788 + LINC00469 NNNATACATAATGAAAACCC NGG 2 0.4875 Tier III
24 NC_000002.12 2 144933723 - TEX41 NNNATACATATTGTAAACCC NGG 2 0.3467 Tier III
25 NC_000001.11 1 221065785 - LOC101929750 NNNAGACCTATTGAAAACAC NGG 2 0.2743 Tier III
26 NC_000002.12 2 227260382 + MFF-DT NNNCTACATATTGAAAACCC NGG 2 0.2294 Tier III
27 NC_000008.11 8 25669499 + LOC107986933 NNNATACCTATTGAACACCC NGG 1 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 113523366 - Emc3 NNNATAACTTTTGAAAACCC NGG 2 0.6618 Tier II
2 NC_000082.6 16 37801647 + Fstl1 NNNCTACCTATTAAAAACCC NGG 2 0.3258 Tier II
3 NC_000075.6 9 88807335 - Gm10634 NNNATACCTAGAGAAAACCC NGG 2 0.32 Tier II
4 NC_000075.6 9 88876397 + Gm2396 NNNATACCTAGAGAAAACCC NGG 2 0.32 Tier II
5 NC_000067.6 1 188690012 - Ush2a NNNATACCTCATGAAAACCC NGG 2 0.25 Tier II
6 NC_000069.6 3 89776905 + Ube2q1 NNNATAGCTATTGGAAACCC NGG 2 0.2196 Tier II
7 NC_000072.6 6 40447181 - Wee2 NNNATACCTAGTGAAAACTC NGG 2 0.1846 Tier II
8 NC_000077.6 11 95072642 - Itga3 NNNATACCTACTGAAAAGCC NGG 2 0.0889 Tier II
9 NC_000075.6 9 88619337 + Gm26567 NNNATACCTAGAGAAAACCC NGG 2 0.32 Tier III
10 NC_000075.6 9 88619337 + Gm29415 NNNATACCTAGAGAAAACCC NGG 2 0.32 Tier III
11 NC_000075.6 9 88876397 + Gm26611 NNNATACCTAGAGAAAACCC NGG 2 0.32 Tier III
12 NC_000075.6 9 89123154 + 4930579C12Rik NNNATACCTAGAGAAAACCC NGG 2 0.32 Tier III
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Other clones with same target sequence:

(none)