Construct: sgRNA BRDN0001149544
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCGGCCCTCCAAGTACACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PKD1 (5310)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76841
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
2106855 |
+ |
PKD1 |
NNNGCCCTCCAAGTACACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
171456756 |
+ |
FGF18 |
NNNGCCCTTCAAGTACACGA |
NGG |
2 |
0.4922 |
Tier I |
| 3 |
NC_000016.10 |
16 |
15134252 |
+ |
PDXDC1 |
NNNGCCCTCCAGGTACACGT |
NGG |
1 |
0.7222 |
Tier II |
| 4 |
NC_000006.12 |
6 |
118310182 |
+ |
SLC35F1 |
NNNGACCTCCAAGTACACTT |
NGG |
2 |
0.381 |
Tier II |
| 5 |
NC_000016.10 |
16 |
53124865 |
+ |
CHD9 |
NNNGCCCTCCCACTACACGT |
NGG |
2 |
0.1684 |
Tier II |
| 6 |
NC_000012.12 |
12 |
53974893 |
- |
HOXC11 |
NNNGCCCTCCAGGTACACGT |
NCG |
2 |
0.0774 |
Tier II |
| 7 |
NC_000014.9 |
14 |
23544558 |
+ |
THTPA |
NNNGCCCTCCATGTACACGT |
NTG |
2 |
0.013 |
Tier II |
| 8 |
NC_000014.9 |
14 |
23544558 |
+ |
ZFHX2 |
NNNGCCCTCCATGTACACGT |
NTG |
2 |
0.013 |
Tier II |
| 9 |
NC_000016.10 |
16 |
14925827 |
- |
PKD1P3 |
NNNGCCCTCCAGGTACACGT |
NGG |
1 |
0.7222 |
Tier III |
| 10 |
NC_000016.10 |
16 |
14925827 |
- |
LOC100288162 |
NNNGCCCTCCAGGTACACGT |
NGG |
1 |
0.7222 |
Tier III |
| 11 |
NC_000016.10 |
16 |
14925827 |
- |
PKD1P3-NPIPA1 |
NNNGCCCTCCAGGTACACGT |
NGG |
1 |
0.7222 |
Tier III |
| 12 |
NC_000016.10 |
16 |
15134252 |
+ |
PKD1P6 |
NNNGCCCTCCAGGTACACGT |
NGG |
1 |
0.7222 |
Tier III |
| 13 |
NC_000016.10 |
16 |
16324478 |
- |
PKD1P1 |
NNNGCCCTCCAGGTACACGT |
NGG |
1 |
0.7222 |
Tier III |
| 14 |
NC_000016.10 |
16 |
16324478 |
- |
LOC131696449 |
NNNGCCCTCCAGGTACACGT |
NGG |
1 |
0.7222 |
Tier III |
| 15 |
NC_000016.10 |
16 |
16368766 |
- |
PKD1P2 |
NNNGCCCTCCAGGTACACGT |
NGG |
1 |
0.7222 |
Tier III |
| 16 |
NC_000016.10 |
16 |
18344190 |
+ |
PKD1P4 |
NNNGCCCTCCAGGTACACGT |
NGG |
1 |
0.7222 |
Tier III |
| 17 |
NC_000016.10 |
16 |
18344190 |
+ |
PKD1P4-NPIPA8 |
NNNGCCCTCCAGGTACACGT |
NGG |
1 |
0.7222 |
Tier III |
| 18 |
NC_000016.10 |
16 |
18383416 |
+ |
PKD1P5 |
NNNGCCCTCCAGGTACACGT |
NGG |
1 |
0.7222 |
Tier III |
| 19 |
NC_000016.10 |
16 |
18383416 |
+ |
PKD1P5-LOC105376752 |
NNNGCCCTCCAGGTACACGT |
NGG |
1 |
0.7222 |
Tier III |
| 20 |
NC_000006.12 |
6 |
118310182 |
+ |
LOC124901387 |
NNNGACCTCCAAGTACACTT |
NGG |
2 |
0.381 |
Tier III |
| 21 |
NC_000012.12 |
12 |
53974893 |
- |
HOTAIR |
NNNGCCCTCCAGGTACACGT |
NCG |
2 |
0.0774 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
24577613 |
- |
Pkd1 |
NNNACCCTCCAAGTACACAT |
NGG |
2 |
0.6429 |
Tier I |
| 2 |
NC_000067.6 |
1 |
168329398 |
- |
Pbx1 |
NNNGCCTTCCAAGTACACAT |
NGG |
2 |
0.5804 |
Tier II |
| 3 |
NC_000083.6 |
17 |
49533710 |
- |
Daam2 |
NNNGCCCTTCAAGAACACGT |
NGG |
2 |
0.5417 |
Tier II |
| 4 |
NC_000086.7 |
X |
20121149 |
+ |
Slc9a7 |
NNNTCCCTCCAAGTACACTT |
NGG |
2 |
0.2424 |
Tier II |
| 5 |
NC_000081.6 |
15 |
27816253 |
+ |
Trio |
NNNGCCCTCCAAGTTCACGT |
NCG |
2 |
0.0214 |
Tier II |
| 6 |
NC_000077.6 |
11 |
75774537 |
+ |
Doc2b |
NNNGCCCTCCAAGTGCACGT |
NGT |
2 |
0.0105 |
Tier II |
| 7 |
NC_000074.6 |
8 |
128340771 |
- |
A930035F09Rik |
NNNACCCTCCAAGTACACGT |
NGG |
1 |
0.9 |
Tier III |
| 8 |
NC_000080.6 |
14 |
54211196 |
+ |
Tcra |
NNNGCCCTCCAGGTCCACGT |
NGG |
2 |
0.197 |
Tier III |
Other clones with same target sequence:
(none)
