Construct: sgRNA BRDN0001162180
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTGCGGAAACCATACTCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- UHMK1 (127933)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77181
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
162498209 |
- |
UHMK1 |
NNNGCGGAAACCATACTCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
77489672 |
+ |
SEPTIN9 |
NNNGGGGAAACCATACTCTG |
NGG |
2 |
0.4 |
Tier II |
| 3 |
NC_000004.12 |
4 |
21596911 |
+ |
KCNIP4 |
NNNGCGGAAACCATCCTCCG |
NGG |
2 |
0.1223 |
Tier II |
| 4 |
NC_000006.12 |
6 |
41153600 |
- |
TREML1 |
NNNGCGGAAACCAAACTGGG |
NGG |
2 |
0.0825 |
Tier II |
| 5 |
NC_000004.12 |
4 |
21596911 |
+ |
LOC105374516 |
NNNGCGGAAACCATCCTCCG |
NGG |
2 |
0.1223 |
Tier III |
| 6 |
NC_000001.11 |
1 |
226914448 |
- |
LOC107985354 |
NNNGCGGAAACCATACAGGG |
NGG |
2 |
0.0711 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
170215033 |
+ |
Uhmk1 |
NNNGCGGAAACCATACTCCG |
NGG |
1 |
0.4483 |
Tier I |
| 2 |
NC_000074.6 |
8 |
105336709 |
- |
Fhod1 |
NNNGCGGAAGCCATCCTCGG |
NGG |
2 |
0.1515 |
Tier I |
| 3 |
NC_000067.6 |
1 |
163915781 |
- |
Kifap3 |
NNNGCGGAATCCATACGCGG |
NGG |
2 |
0.1038 |
Tier I |
| 4 |
NC_000070.6 |
4 |
152130040 |
- |
Espn |
NNNGTGGAAACCATACTGGG |
NGG |
2 |
0.0848 |
Tier II |
| 5 |
NC_000071.6 |
5 |
148798876 |
+ |
Gm36405 |
NNNGTGGAAACCAAACTCGG |
NGG |
2 |
0.3939 |
Tier III |
Other clones with same target sequence:
(none)
