Construct: sgRNA BRDN0001162182
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTATCGAGGTCACTGAACAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EEF2K (29904)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77854
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
22260473 |
- |
EEF2K |
NNNTCGAGGTCACTGAACAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
50216070 |
- |
TENT4B |
NNNTCGATGTCACTGAACAC |
NGA |
2 |
0.0434 |
Tier I |
| 3 |
NC_000016.10 |
16 |
12194337 |
- |
SNX29 |
NNNTCAAGGTCACAGAACAC |
NGG |
2 |
0.619 |
Tier II |
| 4 |
NC_000012.12 |
12 |
128933245 |
+ |
GLT1D1 |
NNNCCGACGTCACTGAACAC |
NGG |
2 |
0.3982 |
Tier II |
| 5 |
NC_000020.11 |
20 |
14684482 |
- |
MACROD2 |
NNNTTGATGTCACTGAACAC |
NGG |
2 |
0.3977 |
Tier II |
| 6 |
NC_000008.11 |
8 |
141393080 |
+ |
PTP4A3 |
NNNAGGAGGTCACTGAACAC |
NGG |
2 |
0.2857 |
Tier II |
| 7 |
NC_000017.11 |
17 |
42839653 |
- |
PSME3 |
NNNTCGAGGTCACACAACAC |
NGG |
2 |
0.1688 |
Tier II |
| 8 |
NC_000012.12 |
12 |
24382560 |
+ |
SOX5 |
NNNTGGAGGTCACTGAGCAC |
NGG |
2 |
0.1059 |
Tier II |
| 9 |
NC_000018.10 |
18 |
46731458 |
+ |
ST8SIA5 |
NNNTAGAGGTCACTGAGCAC |
NGG |
2 |
0.1008 |
Tier II |
| 10 |
NC_000020.11 |
20 |
36234872 |
- |
LOC105372602 |
NNNTCCAGGACACTGAACAC |
NGG |
2 |
0.5844 |
Tier III |
| 11 |
NC_000023.11 |
X |
39018817 |
- |
LOC124905177 |
NNNTCGAGGTCACTGAATAC |
NAG |
2 |
0.1667 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
120887870 |
- |
Eef2k |
NNNTCGAGGTCACCAAACAC |
NGG |
2 |
0.2689 |
Tier I |
| 2 |
NC_000075.6 |
9 |
100468413 |
- |
Il20rb |
NNNTGGAGGTCACTGAACAT |
NGG |
2 |
0.18 |
Tier I |
| 3 |
NC_000081.6 |
15 |
58990402 |
+ |
Mtss1 |
NNNTCGAGCACACTGAACAC |
NGG |
2 |
0.4615 |
Tier II |
| 4 |
NC_000070.6 |
4 |
85825787 |
- |
Adamtsl1 |
NNNTTGAGGCCACTGAACAC |
NGG |
2 |
0.3394 |
Tier II |
| 5 |
NC_000077.6 |
11 |
120238918 |
- |
Bahcc1 |
NNNTGGAGGGCACTGAACAC |
NGG |
2 |
0.3 |
Tier II |
| 6 |
NC_000072.6 |
6 |
114433932 |
- |
Hrh1 |
NNNACGAGGTCACTGAAAAC |
NGG |
2 |
0.2564 |
Tier II |
| 7 |
NC_000079.6 |
13 |
108126115 |
+ |
Ndufaf2 |
NNNTCAAGGTGACTGAACAC |
NGG |
2 |
0.25 |
Tier II |
| 8 |
NC_000067.6 |
1 |
89482287 |
- |
Agap1 |
NNNTCGATGTCACTGAACAG |
NGG |
2 |
0.0368 |
Tier II |
| 9 |
NC_000072.6 |
6 |
89669742 |
+ |
Txnrd3 |
NNNTCTAGGTCACTGAACAC |
NGC |
2 |
0.0148 |
Tier II |
| 10 |
NC_000081.6 |
15 |
58990402 |
+ |
Gm41330 |
NNNTCGAGCACACTGAACAC |
NGG |
2 |
0.4615 |
Tier III |
| 11 |
NC_000080.6 |
14 |
76832276 |
- |
Gm30365 |
NNNTCGGGGTCACTGAAGAC |
NGG |
2 |
0.0941 |
Tier III |
| 12 |
NC_000076.6 |
10 |
24360509 |
- |
Gm15270 |
NNNTGGAGGTCACTGAACAG |
NGG |
2 |
0.0353 |
Tier III |
Other clones with same target sequence:
(none)
