Construct: sgRNA BRDN0001162198
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCGGGTGAATATGTATGACA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- HIPK2 (28996)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000007.14 | 7 | 139620508 | - | HIPK2 | NNNGGTGAATATGTATGACA | NGG | 0 | 1.0 | Tier I |
2 | NC_000012.12 | 12 | 122801333 | - | CCDC62 | NNNGGTGAATATGGATGACA | NCG | 2 | 0.0 | Tier I |
3 | NC_000001.11 | 1 | 65868044 | + | PDE4B | NNNGGTAAATATGTAAGACA | NGG | 2 | 0.9091 | Tier II |
4 | NC_000006.12 | 6 | 72643991 | + | KCNQ5 | NNNGGTGGATATGTATGACA | NGG | 1 | 0.7333 | Tier II |
5 | NC_000011.10 | 11 | 14643031 | + | PSMA1 | NNNGGCGGATATGTATGACA | NGG | 2 | 0.6667 | Tier II |
6 | NC_000016.10 | 16 | 29803692 | - | KIF22 | NNNGGAGAATATGAATGACA | NGG | 2 | 0.5365 | Tier II |
7 | NC_000022.11 | 22 | 30187212 | - | HORMAD2 | NNNAGTTAATATGTATGACA | NGG | 2 | 0.5143 | Tier II |
8 | NC_000001.11 | 1 | 35075132 | - | ZMYM1 | NNNGGTCATTATGTATGACA | NGG | 2 | 0.4125 | Tier II |
9 | NC_000018.10 | 18 | 27168677 | + | CHST9 | NNNGTTGAATATGTAAGACA | NGG | 2 | 0.2727 | Tier II |
10 | NC_000023.11 | X | 112070384 | - | TRPC5 | NNNGGGGAATATGTATGAAA | NGG | 2 | 0.2449 | Tier II |
11 | NC_000013.11 | 13 | 23795992 | - | MIPEP | NNNTGTGTATATGTATGACA | NGG | 2 | 0.1558 | Tier II |
12 | NC_000009.12 | 9 | 112125988 | - | SUSD1 | NNNGGTGAATATGCATGAAA | NGG | 2 | 0.1224 | Tier II |
13 | NC_000011.10 | 11 | 16039097 | + | SOX6 | NNNGGTAAATATGTATGACA | NGA | 2 | 0.0694 | Tier II |
14 | NC_000001.11 | 1 | 92576174 | + | EVI5 | NNNGGTAAATATGTATGACA | NGT | 2 | 0.0161 | Tier II |
15 | NC_000022.11 | 22 | 40194109 | + | TNRC6B | NNNGGTGAATATGCATGACA | NTG | 2 | 0.0111 | Tier II |
16 | NC_000003.12 | 3 | 47076784 | + | SETD2 | NNNGGTGAATATGTATGAGA | NGA | 2 | 0.0087 | Tier II |
17 | NC_000003.12 | 3 | 192171680 | - | FGF12 | NNNGGTGAATATGTCTGACA | NGT | 2 | 0.0044 | Tier II |
18 | NC_000004.12 | 4 | 153549775 | - | TMEM131L | NNNGGTGAATTTGGATGACA | NGG | 2 | 0.0 | Tier II |
19 | NC_000013.11 | 13 | 65862311 | - | LOC105370241 | NNNGGCAAATATGTATGACA | NGG | 2 | 0.9091 | Tier III |
20 | NC_000015.10 | 15 | 34783070 | + | GJD2-DT | NNNGCTAAATATGTATGACA | NGG | 2 | 0.7857 | Tier III |
21 | NC_000006.12 | 6 | 72643991 | + | KCNQ5-IT1 | NNNGGTGGATATGTATGACA | NGG | 1 | 0.7333 | Tier III |
22 | NC_000020.11 | 20 | 49347527 | - | LOC105372649 | NNNGGCGAATGTGTATGACA | NGG | 2 | 0.5909 | Tier III |
23 | NC_000022.11 | 22 | 30187212 | - | LOC105372988 | NNNAGTTAATATGTATGACA | NGG | 2 | 0.5143 | Tier III |
24 | NC_000004.12 | 4 | 14586172 | - | LINC00504 | NNNGGTAAATATGTATCACA | NGG | 2 | 0.2353 | Tier III |
25 | NC_000011.10 | 11 | 4195860 | - | LINC02749 | NNNTGTGTATATGTATGACA | NGG | 2 | 0.1558 | Tier III |
26 | NC_000014.9 | 14 | 44028569 | + | LINC02307 | NNNGTTGAATATGTATGACA | NAG | 2 | 0.0778 | Tier III |
27 | NC_000011.10 | 11 | 16039097 | + | LOC105376572 | NNNGGTAAATATGTATGACA | NGA | 2 | 0.0694 | Tier III |
28 | NC_000005.10 | 5 | 12690233 | - | LINC01194 | NNNGGTGAATATGTATGACT | NTG | 2 | 0.0234 | Tier III |
29 | NC_000001.11 | 1 | 92576174 | + | LOC107985727 | NNNGGTAAATATGTATGACA | NGT | 2 | 0.0161 | Tier III |
30 | NC_000016.10 | 16 | 59933527 | - | LINC02141 | NNNGGTGAATCTGTATGACA | NGC | 2 | 0.0089 | Tier III |
31 | NC_000016.10 | 16 | 59933527 | - | LOC105371300 | NNNGGTGAATCTGTATGACA | NGC | 2 | 0.0089 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000072.6 | 6 | 38737465 | - | Hipk2 | NNNGGTGAATATGTATGACA | NAG | 1 | 0.2593 | Tier I |
2 | NC_000072.6 | 6 | 87277052 | + | Antxr1 | NNNGGTAAATATGTAAGACA | NGG | 2 | 0.9091 | Tier II |
3 | NC_000071.6 | 5 | 96763376 | - | Fras1 | NNNAATGAATATGTATGACA | NGG | 2 | 0.78 | Tier II |
4 | NC_000068.7 | 2 | 10471510 | + | Sfmbt2 | NNNGGAGATTATGTATGACA | NGG | 2 | 0.52 | Tier II |
5 | NC_000076.6 | 10 | 27412431 | - | Lama2 | NNNAGTGAATATGTATGGCA | NGG | 2 | 0.36 | Tier II |
6 | NC_000072.6 | 6 | 8729685 | + | Ica1 | NNNTGTGAATATATATGACA | NGG | 2 | 0.3357 | Tier II |
7 | NC_000070.6 | 4 | 139364036 | - | Emc1 | NNNGGTGAAGATGTGTGACA | NGG | 2 | 0.325 | Tier II |
8 | NC_000082.6 | 16 | 44526509 | - | Boc | NNNGGGGAATATGTATGTCA | NGG | 2 | 0.2857 | Tier II |
9 | NC_000068.7 | 2 | 124262751 | + | Sema6d | NNNGATGAATATGTATCACA | NGG | 2 | 0.2039 | Tier II |
10 | NC_000069.6 | 3 | 76108623 | - | Fstl5 | NNNGATGAATATGTATCACA | NGG | 2 | 0.2039 | Tier II |
11 | NC_000067.6 | 1 | 139327671 | - | Crb1 | NNNGGTGAATATATTTGACA | NGG | 2 | 0.1846 | Tier II |
12 | NC_000068.7 | 2 | 60679547 | - | Itgb6 | NNNGGTGAAAATGTATGCCA | NGG | 2 | 0.1633 | Tier II |
13 | NC_000068.7 | 2 | 7389596 | + | Celf2 | NNNGGTGACTATGTATCACA | NGG | 2 | 0.1345 | Tier II |
14 | NC_000079.6 | 13 | 53245299 | + | Ror2 | NNNGGTGAATATGTGTGCCA | NGG | 2 | 0.1238 | Tier II |
15 | NC_000072.6 | 6 | 136128553 | - | Grin2b | NNNGGTGAATATGTTTGATA | NGG | 2 | 0.0923 | Tier II |
16 | NC_000086.7 | X | 92564634 | - | Mageb18 | NNNGGTGGATATGTATGACA | NGA | 2 | 0.0509 | Tier II |
17 | NC_000081.6 | 15 | 22590108 | - | Cdh18 | NNNGGTGAATATGTATGAGA | NTG | 2 | 0.0049 | Tier II |
18 | NC_000069.6 | 3 | 21781457 | + | 7530428D23Rik | NNNGGAGAATATGTGTGACA | NGG | 2 | 0.5633 | Tier III |
19 | NC_000078.6 | 12 | 88286247 | - | Gm7104 | NNNGGTGAAGATGTAGGACA | NGG | 2 | 0.1731 | Tier III |
20 | NC_000073.6 | 7 | 138523524 | - | Gm30751 | NNNGGTGAATATCTATGGCA | NGG | 2 | 0.1684 | Tier III |