Construct: sgRNA BRDN0001162199

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCCAGAATACTTAACAGCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCLK2 (166614)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75701

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 150198041 - DCLK2 NNNAGAATACTTAACAGCTG NGG 0 1.0 Tier I
2 NC_000006.12 6 73617878 - SLC17A5 NNNAGAAAACTTAACAACTG NGG 2 0.7467 Tier II
3 NC_000006.12 6 125900257 - NCOA7 NNNACAATACTTAACAACTG NGG 2 0.7333 Tier II
4 NC_000019.10 19 37320652 + ZNF875 NNNAGAAGACTCAACAGCTG NGG 2 0.6947 Tier II
5 NC_000004.12 4 87518268 - SPARCL1 NNNAGAAGAATTAACAGCTG NGG 2 0.6356 Tier II
6 NC_000004.12 4 24538165 + DHX15 NNNAAAATACTTAACAGCTT NGG 2 0.6067 Tier II
7 NC_000010.11 10 98901814 - HPSE2 NNNTGAATATTTAACAGCTG NGG 2 0.5989 Tier II
8 NC_000023.11 X 112006592 - TRPC5 NNNAGAATGATTAACAGCTG NGG 2 0.5778 Tier II
9 NC_000005.10 5 41002855 + MROH2B NNNAGGAAACTTAACAGCTG NGG 2 0.5714 Tier II
10 NC_000007.14 7 96197215 + SLC25A13 NNNAGAAATCTTAACAGCTG NGG 2 0.48 Tier II
11 NC_000001.11 1 36352910 + STK40 NNNAGAATGCTTAGCAGCTG NGG 2 0.3111 Tier II
12 NC_000003.12 3 188703520 - LPP NNNAGAATACTTAACACCTG NGG 1 0.2353 Tier II
13 NC_000009.12 9 20593009 - MLLT3 NNNATAATACTTAACAGCTT NGG 2 0.21 Tier II
14 NC_000007.14 7 27590671 - HIBADH NNNAGAATGCTTTACAGCTG NGG 2 0.2 Tier II
15 NC_000009.12 9 92468787 + ASPN NNNATACTACTTAACAGCTG NGG 2 0.1313 Tier II
16 NC_000009.12 9 92468787 + CENPP NNNATACTACTTAACAGCTG NGG 2 0.1313 Tier II
17 NC_000011.10 11 17887843 + SERGEF NNNAGAATACTTTACAGCAG NGG 2 0.0857 Tier II
18 NC_000003.12 3 8679886 + SSUH2 NNNAGAATTCTTAAGAGCTG NGG 2 0.03 Tier II
19 NC_000009.12 9 32992724 + APTX NNNAGAACACTTAACAGCTG NGT 2 0.0161 Tier II
20 NC_000021.9 21 35486873 - LOC100506403 NNNGGAACACTTAACAGCTG NGG 2 0.625 Tier III
21 NC_000002.12 2 89128557 - IGK NNNAGAATACATTACAGCTG NGG 2 0.225 Tier III
22 NC_000002.12 2 90053508 + IGK NNNAGAATACATTACAGCTG NGG 2 0.225 Tier III
23 NC_000002.12 2 120465666 - LINC01101 NNNAGAATATTTAACAGCTG NGT 2 0.0152 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 37971835 - Ubr5 NNNAGAAGACTTAACAGCTG NGG 1 0.7333 Tier I
2 NC_000079.6 13 23934643 + H2ac1 NNNAGAATACTTGACAGCTG NGG 1 0.6522 Tier I
3 NC_000082.6 16 32620492 + Tfrc NNNAGAATACTTAACATCTT NGG 2 0.175 Tier I
4 NC_000069.6 3 86831796 + Dclk2 NNNAGAATACTTCGCAGCTG NGG 2 0.0982 Tier I
5 NC_000069.6 3 75087795 + Zbbx NNNAAAACACTTAACAGCTG NGG 2 0.8667 Tier II
6 NC_000085.6 19 31203873 + Prkg1 NNNAGAAAACTTAACAGCTA NGG 2 0.75 Tier II
7 NC_000079.6 13 103865686 + Erbin NNNAGAAAACTTAACAACTG NGG 2 0.7467 Tier II
8 NC_000073.6 7 115578666 + Sox6 NNNAGAGTATTTAACAGCTG NGG 2 0.6644 Tier II
9 NC_000070.6 4 148532472 + Mtor NNNAGAACACTTGACAGCTG NGG 2 0.6522 Tier II
10 NC_000070.6 4 140145705 - Igsf21 NNNTGAATACTCAACAGCTG NGG 2 0.6029 Tier II
11 NC_000070.6 4 15973060 + Nbn NNNAGAATACTGAACAGCTG NGG 1 0.5 Tier II
12 NC_000070.6 4 77466081 + Ptprd NNNAGAAAACTTAGCAGCTG NGG 2 0.3733 Tier II
13 NC_000080.6 14 75120589 - Lrrc63 NNNAGAATGCTTAGCAGCTG NGG 2 0.3111 Tier II
14 NC_000072.6 6 52742515 - Tax1bp1 NNNGGATTACTTAACAGCTG NGG 2 0.2734 Tier II
15 NC_000069.6 3 129924897 + Mcub NNNAGAATACTTAAAAACTG NGG 2 0.2074 Tier II
16 NC_000075.6 9 72843356 + Prtg NNNAGAATACTTTACAGCTG NAG 2 0.0778 Tier II
17 NC_000075.6 9 16327761 + Fat3 NNNAGAATACTTAACACCTG NAG 2 0.061 Tier II
18 NC_000072.6 6 118738820 + Cacna1c NNNAGAATACTTCACAGCTG NAG 2 0.0546 Tier II
19 NC_000080.6 14 122616179 + Pcca NNNAGAATTCTTAACAGCTG NGA 2 0.0417 Tier II
20 NC_000072.6 6 142112095 - Slco1a6 NNNAGAATACTTAGTAGCTG NGG 2 0.0311 Tier II
21 NC_000070.6 4 118071709 + St3gal3 NNNACAATACTTAACAGCTG NTG 2 0.0306 Tier II
22 NC_000069.6 3 67001249 + Rsrc1 NNNCGAATACTTAATAGCTG NGG 2 0.0235 Tier II
23 NC_000075.6 9 56490606 - Hmg20a NNNAGAACACTTAACAGCTG NGC 2 0.0222 Tier II
24 NC_000068.7 2 31914312 - Lamc3 NNNAGAGTACTTAACAGCTG NGC 2 0.0157 Tier II
25 NC_000085.6 19 32693330 + Atad1 NNNAGAATTCTTAACAGCTG NGT 2 0.0097 Tier II
26 NC_000070.6 4 72159729 + Tle1 NNNAGAATACTTCACAGCTG NGT 2 0.0034 Tier II
27 NC_000081.6 15 37971835 - Gm49085 NNNAGAAGACTTAACAGCTG NGG 1 0.7333 Tier III
28 NC_000079.6 13 23934643 + Gm33516 NNNAGAATACTTGACAGCTG NGG 1 0.6522 Tier III
29 NC_000080.6 14 79916979 + Gm6999 NNNAGAATTCTTAACAGCTC NGG 2 0.2571 Tier III
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Other clones with same target sequence:

(none)