Construct: sgRNA BRDN0001162211
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGCCTCTGCCATGACATAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ADCK5 (203054)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77320
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
144383162 |
- |
ADCK5 |
NNNCCTCTGCCATGACATAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
111424829 |
+ |
OVGP1 |
NNNCCTCAGCCATGAAATAG |
NGG |
2 |
0.8 |
Tier II |
| 3 |
NC_000006.12 |
6 |
46357127 |
+ |
RCAN2 |
NNNCCTCTGCCAAGACATAA |
NGG |
2 |
0.649 |
Tier II |
| 4 |
NC_000015.10 |
15 |
38521525 |
- |
RASGRP1 |
NNNCCTTTGCCATGGCATAG |
NGG |
2 |
0.5281 |
Tier II |
| 5 |
NC_000001.11 |
1 |
100367019 |
+ |
CDC14A |
NNNCCTCTGCCAAGACAAAG |
NGG |
2 |
0.4615 |
Tier II |
| 6 |
NC_000011.10 |
11 |
14154308 |
+ |
SPON1 |
NNNCTTCTGCCATGACATAC |
NGG |
2 |
0.2727 |
Tier II |
| 7 |
NC_000014.9 |
14 |
72010140 |
- |
RGS6 |
NNNCCACTGCCATGACATAG |
NAG |
2 |
0.2247 |
Tier II |
| 8 |
NC_000007.14 |
7 |
132168893 |
- |
PLXNA4 |
NNNGCTCTGCCATGACACAG |
NGG |
2 |
0.2143 |
Tier II |
| 9 |
NC_000003.12 |
3 |
149895242 |
- |
RNF13 |
NNNCCTCTGCCATGAATTAG |
NGG |
2 |
0.1333 |
Tier II |
| 10 |
NC_000012.12 |
12 |
129532374 |
- |
TMEM132D |
NNNCCTCTGGCATGACAGAG |
NGG |
2 |
0.1296 |
Tier II |
| 11 |
NC_000014.9 |
14 |
79776667 |
+ |
NRXN3 |
NNNCCTCTGCCTTGACATAG |
NTG |
2 |
0.013 |
Tier II |
| 12 |
NC_000005.10 |
5 |
135695999 |
+ |
SLC25A48 |
NNNCATCTGCCATGACATAG |
NGC |
2 |
0.0127 |
Tier II |
| 13 |
NC_000009.12 |
9 |
3010884 |
+ |
CARM1P1 |
NNNACACTGCCATGACATAG |
NGG |
2 |
0.7298 |
Tier III |
| 14 |
NC_000006.12 |
6 |
46357127 |
+ |
LOC101926915 |
NNNCCTCTGCCAAGACATAA |
NGG |
2 |
0.649 |
Tier III |
| 15 |
NC_000016.10 |
16 |
2951362 |
+ |
LOC105371055 |
NNNCCTCTGCCAAGACAGAG |
NGG |
2 |
0.2308 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
48868241 |
- |
Kcnip4 |
NNNCCTTTGCCATGACACAG |
NGG |
2 |
0.3482 |
Tier II |
| 2 |
NC_000069.6 |
3 |
35963867 |
+ |
Mccc1 |
NNNCCTCTCCCATGACACAG |
NGG |
2 |
0.2308 |
Tier II |
| 3 |
NC_000076.6 |
10 |
115083059 |
- |
Tph2 |
NNNCCTCTTCCATGACATAC |
NGG |
2 |
0.2286 |
Tier II |
| 4 |
NC_000071.6 |
5 |
112775501 |
+ |
Myo18b |
NNNCCTCTCCCATGACATGG |
NGG |
2 |
0.2019 |
Tier II |
| 5 |
NC_000073.6 |
7 |
25776827 |
+ |
Axl |
NNNCCTCTGCCAGGACAAAG |
NGG |
2 |
0.1739 |
Tier II |
| 6 |
NC_000077.6 |
11 |
43852835 |
- |
Adra1b |
NNNCTTCTGCCAGGACATAG |
NGG |
2 |
0.166 |
Tier II |
| 7 |
NC_000067.6 |
1 |
107554431 |
- |
Serpinb10 |
NNNCCTCTGCCTTGACACAG |
NGG |
2 |
0.1429 |
Tier II |
| 8 |
NC_000071.6 |
5 |
20602849 |
- |
Magi2 |
NNNCCTCTGCCATGGCTTAG |
NGG |
2 |
0.0867 |
Tier II |
| 9 |
NC_000068.7 |
2 |
33609194 |
- |
Lmx1b |
NNNCCTCTGCCAGGACATAG |
NAG |
2 |
0.0676 |
Tier II |
| 10 |
NC_000072.6 |
6 |
141285285 |
+ |
Pde3a |
NNNCCACTGCCATGACATAG |
NTG |
2 |
0.0338 |
Tier II |
| 11 |
NC_000077.6 |
11 |
47486141 |
+ |
Sgcd |
NNNCCTCTGCCATGACACAG |
NGA |
2 |
0.0298 |
Tier II |
| 12 |
NC_000081.6 |
15 |
31244185 |
+ |
Dap |
NNNCCTCTGCCATCACATAG |
NTG |
2 |
0.0167 |
Tier II |
| 13 |
NC_000086.7 |
X |
101854589 |
+ |
Nhsl2 |
NNNCCTCTGCCATGACATTG |
NGT |
2 |
0.0087 |
Tier II |
| 14 |
NC_000073.6 |
7 |
90642574 |
- |
Dlg2 |
NNNCCTCTGCCATGACATAG |
NCC |
2 |
0.0 |
Tier II |
| 15 |
NC_000083.6 |
17 |
79443458 |
+ |
Gm32954 |
NNNCATCTGCCCTGACATAG |
NGG |
2 |
0.1504 |
Tier III |
Other clones with same target sequence:
(none)
