Construct: sgRNA BRDN0001162217
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTGGCCAGAATGATCATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- UCKL1 (54963)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76346
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
63946217 |
- |
UCKL1 |
NNNGGCCAGAATGATCATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
41027512 |
+ |
UNC5CL |
NNNGGCCAGAATGATCATCT |
NGA |
2 |
0.0486 |
Tier II |
| 3 |
NC_000014.9 |
14 |
36711516 |
+ |
SLC25A21 |
NNNGGCCAGAATGATCATCT |
NTG |
2 |
0.0273 |
Tier II |
| 4 |
NC_000001.11 |
1 |
7527659 |
+ |
CAMTA1 |
NNNGGCCAGAATGCTCATCG |
NGC |
2 |
0.0048 |
Tier II |
| 5 |
NC_000003.12 |
3 |
184524183 |
+ |
LINC01839 |
NNNGACCAGAATGTTCATCG |
NGG |
2 |
0.4622 |
Tier III |
| 6 |
NC_000002.12 |
2 |
698116 |
- |
LOC105373358 |
NNNGGCCAGAATGATCATCT |
NGA |
2 |
0.0486 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
181574707 |
- |
Uckl1 |
NNNGGCCAGAATGATCATTG |
NGG |
1 |
0.4615 |
Tier I |
| 2 |
NC_000071.6 |
5 |
131613805 |
+ |
Auts2 |
NNNGGTCAGAAAGATCATCG |
NGG |
2 |
0.7429 |
Tier II |
| 3 |
NC_000074.6 |
8 |
17458079 |
- |
C030002A05Rik |
NNNGGCCAGTATGATCATAG |
NGG |
2 |
0.3782 |
Tier II |
| 4 |
NC_000074.6 |
8 |
17458079 |
- |
Csmd1 |
NNNGGCCAGTATGATCATAG |
NGG |
2 |
0.3782 |
Tier II |
| 5 |
NC_000073.6 |
7 |
37773888 |
+ |
Zfp536 |
NNNGTCCAGAATAATCATCG |
NGG |
2 |
0.2769 |
Tier II |
| 6 |
NC_000077.6 |
11 |
58096894 |
- |
Cnot8 |
NNNTGCCAGACTGATCATCG |
NGG |
2 |
0.1455 |
Tier II |
| 7 |
NC_000077.6 |
11 |
77002174 |
+ |
Slc6a4 |
NNNGGCCAGAATGACCATAG |
NGG |
2 |
0.1169 |
Tier II |
| 8 |
NC_000072.6 |
6 |
113289928 |
+ |
Cpne9 |
NNNGGCCAGAATGATCATGC |
NGG |
2 |
0.0536 |
Tier II |
| 9 |
NC_000072.6 |
6 |
62929206 |
- |
Gm5001 |
NNNGGTAAGAATGATCATCG |
NGG |
2 |
0.6964 |
Tier III |
| 10 |
NC_000071.6 |
5 |
67108482 |
- |
Gm33167 |
NNNGCCCAGAATGATCACCG |
NGG |
2 |
0.3367 |
Tier III |
| 11 |
NC_000077.6 |
11 |
58096894 |
- |
Gm12247 |
NNNTGCCAGACTGATCATCG |
NGG |
2 |
0.1455 |
Tier III |
Other clones with same target sequence:
(none)
