Construct: sgRNA BRDN0001162218
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTCTCGAACCTCATTGCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCI (5584)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76403
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
170235281 |
- |
PRKCI |
NNNCTCGAACCTCATTGCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
6321361 |
+ |
PPP2R2C |
NNNCTCGAACCTCTTTACAA |
NGG |
2 |
0.4978 |
Tier II |
| 3 |
NC_000021.9 |
21 |
45904007 |
- |
PCBP3 |
NNNCTCTAAACTCATTGCAA |
NGG |
2 |
0.4952 |
Tier II |
| 4 |
NC_000005.10 |
5 |
88214684 |
+ |
TMEM161B |
NNNCTCCATCCTCATTGCAA |
NGG |
2 |
0.4125 |
Tier II |
| 5 |
NC_000019.10 |
19 |
50450789 |
- |
MYBPC2 |
NNNCTCGAACCAGATTGCAA |
NGG |
2 |
0.1091 |
Tier II |
| 6 |
NC_000023.11 |
X |
101539126 |
+ |
PRKCIP1 |
NNNCTCGAACCACATTGTAA |
NGG |
2 |
0.5143 |
Tier III |
| 7 |
NC_000007.14 |
7 |
144213446 |
- |
ARHGEF35-AS1 |
NNNCTCTAACCTCAATGCAA |
NGG |
2 |
0.3308 |
Tier III |
| 8 |
NC_000007.14 |
7 |
144337546 |
+ |
OR2A1-AS1 |
NNNCTCTAACCTCAATGCAA |
NGG |
2 |
0.3308 |
Tier III |
| 9 |
NC_000014.9 |
14 |
106052443 |
+ |
IGH |
NNNCTGGAACCTCATTGCAA |
NTG |
2 |
0.0195 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
31018601 |
- |
Prkci |
NNNCTCGAACCTCACTGCAA |
NGT |
2 |
0.0044 |
Tier I |
| 2 |
NC_000073.6 |
7 |
81151113 |
- |
Slc28a1 |
NNNCTAGAGCCTCATTGCAA |
NGG |
2 |
0.619 |
Tier II |
| 3 |
NC_000076.6 |
10 |
7051072 |
+ |
Ipcef1 |
NNNCTCGAATCTCATTGCAA |
NAG |
2 |
0.244 |
Tier II |
Other clones with same target sequence:
(none)
