Construct: sgRNA BRDN0001162219
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGTAATTCAGACCACACCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKD1 (5587)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77231
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
29663858 |
+ |
PRKD1 |
NNNTAATTCAGACCACACCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
27582598 |
- |
PPFIBP1 |
NNNGAATGCAGACCACACCC |
NGG |
2 |
0.4583 |
Tier II |
| 3 |
NC_000016.10 |
16 |
6944111 |
+ |
RBFOX1 |
NNNTACTTAAGACCACACCC |
NGG |
2 |
0.3896 |
Tier II |
| 4 |
NC_000003.12 |
3 |
105542490 |
+ |
ALCAM |
NNNTAATTCATACCACACCC |
NGG |
1 |
0.3846 |
Tier II |
| 5 |
NC_000006.12 |
6 |
147542922 |
+ |
SAMD5 |
NNNTAGTTCAGCCCACACCC |
NGG |
2 |
0.188 |
Tier II |
| 6 |
NC_000007.14 |
7 |
55383894 |
+ |
LANCL2 |
NNNTAATTCAGCCAACACCC |
NGG |
2 |
0.0921 |
Tier II |
| 7 |
NC_000002.12 |
2 |
235005273 |
- |
SH3BP4 |
NNNCAATTCAGACCACTCCC |
NGG |
2 |
0.0863 |
Tier II |
| 8 |
NC_000007.14 |
7 |
149250887 |
+ |
ZNF212 |
NNNTAATTCAGACCACACCT |
NTG |
2 |
0.0117 |
Tier II |
| 9 |
NC_000003.12 |
3 |
80529840 |
- |
LOC105377177 |
NNNTAATTCAGCCTACACCC |
NGG |
2 |
0.193 |
Tier III |
| 10 |
NC_000023.11 |
X |
33862575 |
+ |
LOC105373153 |
NNNTAGTTCAGCCCACACCC |
NGG |
2 |
0.188 |
Tier III |
| 11 |
NC_000015.10 |
15 |
32180398 |
- |
LOC102724078 |
NNNTAATTCAGACCACAACG |
NGG |
2 |
0.0317 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
50425598 |
+ |
Prkd1 |
NNNTAATTCAGACCACATCC |
NGA |
2 |
0.0446 |
Tier I |
| 2 |
NC_000076.6 |
10 |
45132837 |
- |
Prep |
NNNTAAAACAGACCACACCC |
NGG |
2 |
0.7 |
Tier II |
| 3 |
NC_000076.6 |
10 |
79683797 |
- |
Cdc34 |
NNNTAATTCAGACCACATCC |
NGG |
1 |
0.6429 |
Tier II |
| 4 |
NC_000084.6 |
18 |
71714903 |
+ |
Dcc |
NNNTAATTTAGACCACATCC |
NGG |
2 |
0.5625 |
Tier II |
| 5 |
NC_000082.6 |
16 |
75011183 |
- |
Robo2 |
NNNGAATTCTGACCACACCC |
NGG |
2 |
0.5515 |
Tier II |
| 6 |
NC_000078.6 |
12 |
38502399 |
- |
Dgkb |
NNNTATTTCAGATCACACCC |
NGG |
2 |
0.5 |
Tier II |
| 7 |
NC_000068.7 |
2 |
109258860 |
+ |
Mettl15 |
NNNTAATTCAGACAAAACCC |
NGG |
2 |
0.35 |
Tier II |
| 8 |
NC_000067.6 |
1 |
93584624 |
- |
Farp2 |
NNNTTATTTAGACCACACCC |
NGG |
2 |
0.3182 |
Tier II |
| 9 |
NC_000084.6 |
18 |
78830092 |
+ |
Setbp1 |
NNNTAATGCACACCACACCC |
NGG |
2 |
0.3143 |
Tier II |
| 10 |
NC_000075.6 |
9 |
8119554 |
+ |
Cep126 |
NNNTAATTAAGACCACACCC |
NAG |
2 |
0.2222 |
Tier II |
| 11 |
NC_000084.6 |
18 |
84261368 |
- |
Zfp407 |
NNNTAATTCTGACCACCCCC |
NGG |
2 |
0.1557 |
Tier II |
| 12 |
NC_000074.6 |
8 |
58213730 |
- |
Galntl6 |
NNNTACTTCAGACCACGCCC |
NGG |
2 |
0.0802 |
Tier II |
| 13 |
NC_000071.6 |
5 |
89009442 |
+ |
Slc4a4 |
NNNTAATTCAGACCTCACCT |
NGG |
2 |
0.06 |
Tier II |
| 14 |
NC_000080.6 |
14 |
28792205 |
- |
Gm35217 |
NNNTAAAACAGACCACACCC |
NGG |
2 |
0.7 |
Tier III |
| 15 |
NC_000081.6 |
15 |
41297579 |
+ |
Gm33557 |
NNNTAGTTCAGTCCACACCC |
NGG |
2 |
0.2381 |
Tier III |
| 16 |
NC_000071.6 |
5 |
72567941 |
+ |
Gm34144 |
NNNTAATTAAGACCACACCC |
NAG |
2 |
0.2222 |
Tier III |
| 17 |
NC_000073.6 |
7 |
17356885 |
+ |
Gm32772 |
NNNTAATTAAGACCACACCC |
NAG |
2 |
0.2222 |
Tier III |
Other clones with same target sequence:
(none)
