Construct: sgRNA BRDN0001162220

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGGTTTCCCAACAGCAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XYLB (9942)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77648

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 38365201 + XYLB NNNGTTTCCCAACAGCAACA NGG 0 1.0 Tier I
2 NC_000014.9 14 73206388 + PSEN1 NNNCTTTCCCAACAGCAACA NTG 2 0.0206 Tier I
3 NC_000022.11 22 46184550 - PPARA NNNATTTTCCAACAGCAACA NGG 2 0.7875 Tier II
4 NC_000016.10 16 12274256 - SNX29 NNNGTTTCTAAACAGCAACA NGG 2 0.7583 Tier II
5 NC_000016.10 16 519651 - RAB11FIP3 NNNGTCTCCCAACAGCAACG NGG 2 0.6952 Tier II
6 NC_000004.12 4 153294563 + TRIM2 NNNGTTTCCTAATAGCAACA NGG 2 0.6588 Tier II
7 NC_000008.11 8 66754973 + SGK3 NNNGTTTAACAACAGCAACA NGG 2 0.5571 Tier II
8 NC_000008.11 8 66754973 + C8orf44-SGK3 NNNGTTTAACAACAGCAACA NGG 2 0.5571 Tier II
9 NC_000010.11 10 25496834 - GPR158 NNNGTTACCCAACTGCAACA NGG 2 0.4667 Tier II
10 NC_000003.12 3 156491006 + KCNAB1 NNNGATTCTCAACAGCAACA NGG 2 0.4375 Tier II
11 NC_000003.12 3 31566250 - STT3B NNNGATTCCAAACAGCAACA NGG 2 0.4333 Tier II
12 NC_000009.12 9 4311647 + GLIS3 NNNCTTCCCCAACAGCAACA NGG 2 0.364 Tier II
13 NC_000002.12 2 167426979 + B3GALT1 NNNGTTCCCCAACAGCAAAA NGG 2 0.2946 Tier II
14 NC_000002.12 2 69027144 - ANTXR1 NNNGATGCCCAACAGCAACA NGG 2 0.2941 Tier II
15 NC_000015.10 15 50014127 + ATP8B4 NNNATTTCCCACCAGCAACA NGG 2 0.2368 Tier II
16 NC_000023.11 X 114889311 - HTR2C NNNGTGTCCCAACAGCAACA NAG 2 0.1481 Tier II
17 NC_000011.10 11 126588115 + KIRREL3 NNNGTTTCCCATCAGCAAAA NGG 2 0.1429 Tier II
18 NC_000014.9 14 79634498 - NRXN3 NNNTTTTCCCAAAAGCAACA NGG 2 0.1399 Tier II
19 NC_000018.10 18 58578666 - ALPK2 NNNGTTTCCCAACACCATCA NGG 2 0.1364 Tier II
20 NC_000015.10 15 80576065 - ARNT2 NNNGTTTCCCAACAGAAAGA NGG 2 0.125 Tier II
21 NC_000005.10 5 31992726 - PDZD2 NNNCTTTCCCAAGAGCAACA NGG 2 0.0722 Tier II
22 NC_000001.11 1 25652300 - MAN1C1 NNNGTTTCCCAACAGCAACC NAG 2 0.0589 Tier II
23 NC_000001.11 1 197586177 + DENND1B NNNGTTTCCCAAAAGCTACA NGG 2 0.0513 Tier II
24 NC_000001.11 1 20078560 + PLA2G5 NNNGTTTCCCACCAGCCACA NGG 2 0.0464 Tier II
25 NC_000001.11 1 244502603 - CATSPERE NNNGTTTCCTAACAGCAACA NTG 2 0.0367 Tier II
26 NC_000010.11 10 68102638 - MYPN NNNATTTCCCAACAGCAACA NTG 2 0.0351 Tier II
27 NC_000008.11 8 105570061 - ZFPM2 NNNGTTTCCCAAGAGGAACA NGG 2 0.021 Tier II
28 NC_000013.11 13 42915573 + EPSTI1 NNNGTTTCCCAACAACAACA NGT 2 0.0152 Tier II
29 NC_000014.9 14 39651207 - LOC105370461 NNNGTTTCTCAACAGCATCA NGG 2 0.4375 Tier III
30 NC_000009.12 9 7315021 - LOC105375970 NNNTTTTCCCAGCAGCAACA NGG 2 0.2626 Tier III
31 NC_000011.10 11 126588115 + KIRREL3-AS1 NNNGTTTCCCATCAGCAAAA NGG 2 0.1429 Tier III
32 NC_000008.11 8 7893770 + LOC124901865 NNNGTTTCCCATCACCAACA NGG 2 0.0909 Tier III
33 NC_000015.10 15 25067083 - SNHG14 NNNGTTTCCCAACAGCACAA NGG 2 0.0816 Tier III
34 NC_000015.10 15 25067083 - LOC128966705 NNNGTTTCCCAACAGCACAA NGG 2 0.0816 Tier III
35 NC_000008.11 8 5912649 + LOC105377795 NNNCTTTCCCAACATCAACA NGG 2 0.0756 Tier III
36 NC_000021.9 21 14038055 - LOC105377134 NNNGTTTCCCAAAATCAACA NGG 2 0.0549 Tier III
37 NC_000008.11 8 88531669 + LOC105375629 NNNGTTTCCCCACAGCAAGA NGG 2 0.05 Tier III
38 NC_000008.11 8 88531669 + LOC105375630 NNNGTTTCCCCACAGCAAGA NGG 2 0.05 Tier III
39 NC_000013.11 13 42915573 + LOC124903165 NNNGTTTCCCAACAACAACA NGT 2 0.0152 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 4038010 - Dnase1 NNNGCTTCCCAACAGCAACC NGG 2 0.2273 Tier I
2 NC_000072.6 6 8630722 + Ica1 NNNTTTTCCCAACAGCATCA NGG 2 0.1818 Tier I
3 NC_000073.6 7 45800560 + Lmtk3 NNNGTTTCCCAACAGCGACA NCG 2 0.0189 Tier I
4 NC_000071.6 5 131185314 + Galnt17 NNNGTATCCCAACAGAAACA NGG 2 0.8667 Tier II
5 NC_000068.7 2 157011270 - Soga1 NNNGTTTGCCAACAGCAACA NGG 1 0.6429 Tier II
6 NC_000068.7 2 27678693 - Rxra NNNGTTTCTCAACTGCAACA NGG 2 0.4667 Tier II
7 NC_000068.7 2 18853405 - Pip4k2a NNNCTTACCCAACAGCAACA NGG 2 0.4632 Tier II
8 NC_000067.6 1 5102274 - Atp6v1h NNNAATTCCCAACAGCAACA NGG 2 0.45 Tier II
9 NC_000076.6 10 69544671 + Ank3 NNNATTTCCCAACAGCATCA NGG 2 0.45 Tier II
10 NC_000069.6 3 117618557 + Plppr5 NNNGCTTCCCCACAGCAACA NGG 2 0.4 Tier II
11 NC_000072.6 6 128298901 + Tead4 NNNGTTTCCCCACAGAAACA NGG 2 0.4 Tier II
12 NC_000083.6 17 90699644 - Nrxn1 NNNGTTTTCCAACAGCAAAA NGG 2 0.375 Tier II
13 NC_000085.6 19 30678248 - Prkg1 NNNTTTTCCCAACAGCAACA NGG 1 0.3636 Tier II
14 NC_000084.6 18 67980153 - Ldlrad4 NNNGATTCCCAGCAGCAACA NGG 2 0.3611 Tier II
15 NC_000081.6 15 91529006 + Slc2a13 NNNATTTCCCCACAGCAACA NGG 2 0.36 Tier II
16 NC_000074.6 8 58350680 - Galntl6 NNNCTTGCCCAACAGCAACA NGG 2 0.3114 Tier II
17 NC_000071.6 5 51537820 + Ppargc1a NNNGTTTCCCAACAACAACA NAG 2 0.244 Tier II
18 NC_000083.6 17 33703314 - Marchf2 NNNTTTTCCCAACAGCAACT NGG 2 0.2182 Tier II
19 NC_000070.6 4 145698562 - Zfp980 NNNGTTTCCCAGCACCAACA NGG 2 0.197 Tier II
20 NC_000070.6 4 146192625 - Zfp600 NNNGTTTCCCAGCACCAACA NGG 2 0.197 Tier II
21 NC_000070.6 4 146715250 - Zfp993 NNNGTTTCCCAGCACCAACA NGG 2 0.197 Tier II
22 NC_000070.6 4 147054919 - Rex2 NNNGTTTCCCAGCACCAACA NGG 2 0.197 Tier II
23 NC_000070.6 4 147387641 - Zfp978 NNNGTTTCCCAGCACCAACA NGG 2 0.197 Tier II
24 NC_000070.6 4 147616093 + Zfp979 NNNGTTTCCCAGCACCAACA NGG 2 0.197 Tier II
25 NC_000075.6 9 65466303 - Spg21 NNNGTTTCCCAACAGCACCA NGG 1 0.1905 Tier II
26 NC_000067.6 1 181040369 - 9130409I23Rik NNNTATTCCCAACAGCAACA NGG 2 0.1818 Tier II
27 NC_000078.6 12 108417252 - Eml1 NNNCTTTCCCATCAGCAACA NGG 2 0.1765 Tier II
28 NC_000068.7 2 144406249 + Kat14 NNNGGTTCCCAACACCAACA NGG 2 0.1745 Tier II
29 NC_000069.6 3 87129266 - Kirrel NNNGTCTCCCAACAGCACCA NGG 2 0.1732 Tier II
30 NC_000077.6 11 118655578 - Rbfox3 NNNGTGTCCCAACACCAACA NGG 2 0.1558 Tier II
31 NC_000076.6 10 74090915 + Pcdh15 NNNGTTTTCCAACAGCCACA NGG 2 0.1544 Tier II
32 NC_000081.6 15 55432552 - Col14a1 NNNGATTCCCAACAGTAACA NGG 2 0.1538 Tier II
33 NC_000075.6 9 67300685 + Tln2 NNNGTTTCCCATCAGCAAAA NGG 2 0.1429 Tier II
34 NC_000081.6 15 91308621 + Slc2a13 NNNGTTTCCCAGCAGCCACA NGG 2 0.1275 Tier II
35 NC_000082.6 16 13794633 - Rrn3 NNNATTTCCCAACAGCTACA NGG 2 0.12 Tier II
36 NC_000071.6 5 112374372 - Hps4 NNNGTTTCCCAACACCAGCA NGG 2 0.1091 Tier II
37 NC_000074.6 8 4337704 + Ccl25 NNNGGTTCCCAACATCAACA NGG 2 0.0914 Tier II
38 NC_000076.6 10 122925881 + Ppm1h NNNGATTCCCAACAGCCACA NGG 2 0.0882 Tier II
39 NC_000077.6 11 119999536 + Baiap2 NNNCTTTCCCAAGAGCAACA NGG 2 0.0722 Tier II
40 NC_000079.6 13 55558847 + Fam193b NNNGATTCCCAACAGCTACA NGG 2 0.0667 Tier II
41 NC_000082.6 16 43993307 + Gramd1c NNNGATTCCCAACAGCTACA NGG 2 0.0667 Tier II
42 NC_000083.6 17 29620850 - Rnf8 NNNTTTTCCCAACAGCCACA NGG 2 0.0642 Tier II
43 NC_000067.6 1 58746852 - Cflar NNNGTTTCCCAAAAGCAAGA NGG 2 0.0481 Tier II
44 NC_000074.6 8 107305035 + Nfat5 NNNGTTTCCCAACACCCACA NGG 2 0.0481 Tier II
45 NC_000076.6 10 60042453 - Ascc1 NNNGTTTCCCAACACCTACA NGG 2 0.0364 Tier II
46 NC_000071.6 5 144852172 + Trrap NNNGTTTCCCAACATCCACA NGG 2 0.0252 Tier II
47 NC_000071.6 5 19888687 + Magi2 NNNGTTTCCCAACAGGAAGA NGG 2 0.0192 Tier II
48 NC_000076.6 10 69544671 + Gm33464 NNNATTTCCCAACAGCATCA NGG 2 0.45 Tier III
49 NC_000076.6 10 69544671 + Gm51806 NNNATTTCCCAACAGCATCA NGG 2 0.45 Tier III
50 NC_000075.6 9 43608993 + Gm29909 NNNGTCTCCCAAAAGCAACA NGG 2 0.3497 Tier III
51 NC_000070.6 4 146192625 - Vmn2r-ps17 NNNGTTTCCCAGCACCAACA NGG 2 0.197 Tier III
52 NC_000070.6 4 146192625 - Gm13165 NNNGTTTCCCAGCACCAACA NGG 2 0.197 Tier III
53 NC_000073.6 7 61182262 - A230006K03Rik NNNGTTTCCAAACAGCCACA NGG 2 0.1529 Tier III
54 NC_000071.6 5 112374372 - Gm20636 NNNGTTTCCCAACACCAGCA NGG 2 0.1091 Tier III
55 NC_000073.6 7 36076388 - Gm38991 NNNGTTTCCCACCAGCAGCA NGG 2 0.1053 Tier III
56 NC_000074.6 8 4337704 + LOC108167518 NNNGGTTCCCAACATCAACA NGG 2 0.0914 Tier III
57 NC_000083.6 17 29620850 - Gm28043 NNNTTTTCCCAACAGCCACA NGG 2 0.0642 Tier III
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Other clones with same target sequence:

(none)