Construct: sgRNA BRDN0001162222
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGACTCAAGCGTAAGAGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HIPK2 (28996)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76750
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
139716632 |
- |
HIPK2 |
NNNACTCAAGCGTAAGAGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
102351780 |
- |
GBF1 |
NNNACTCAAGGGTAAGAGAG |
NGG |
2 |
0.1071 |
Tier II |
| 3 |
NC_000007.14 |
7 |
28529549 |
+ |
CREB5 |
NNNACTCAAGAGTAAGAGGG |
NGG |
2 |
0.0938 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
38818930 |
- |
Hipk2 |
NNNACTCAAGCGCAAGAGTG |
NGG |
2 |
0.3644 |
Tier I |
| 2 |
NC_000067.6 |
1 |
127659316 |
- |
Tmem163 |
NNNACTCAAGAGTAAGAGCT |
NGG |
2 |
0.525 |
Tier II |
| 3 |
NC_000072.6 |
6 |
30586681 |
- |
Cpa4 |
NNNACTCAAGCATAAGAGCC |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000084.6 |
18 |
20833085 |
+ |
Trappc8 |
NNNACTCAAGGGTAAGAGCA |
NGG |
2 |
0.2344 |
Tier II |
| 5 |
NC_000077.6 |
11 |
83302248 |
+ |
Ap2b1 |
NNNACTCAAGCCTAAGGGCG |
NGG |
2 |
0.0934 |
Tier II |
Other clones with same target sequence:
(none)
