Construct: sgRNA BRDN0001162224
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGCCAGGATGTGGGTCCGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ADCK5 (203054)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77317
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
144392003 |
- |
ADCK5 |
NNNCAGGATGTGGGTCCGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
120981854 |
- |
HNF1A |
NNNCAGGATGTGGGTACAAG |
NGG |
2 |
0.6923 |
Tier II |
| 3 |
NC_000010.11 |
10 |
49689976 |
+ |
C10orf53 |
NNNAAGGAGGTGGGTCCGAG |
NGG |
2 |
0.5213 |
Tier II |
| 4 |
NC_000012.12 |
12 |
4850296 |
+ |
KCNA6 |
NNNCAGGCTGAGGGTCCGAG |
NGG |
2 |
0.3214 |
Tier II |
| 5 |
NC_000018.10 |
18 |
80193743 |
+ |
PARD6G |
NNNCAGCATGTGGCTCCGAG |
NGG |
2 |
0.2946 |
Tier II |
| 6 |
NC_000005.10 |
5 |
10617353 |
- |
ANKRD33B |
NNNCAGGATGTGGGACAGAG |
NGG |
2 |
0.2702 |
Tier II |
| 7 |
NC_000010.11 |
10 |
71672034 |
- |
CDH23 |
NNNCAGGATGTGGCTCCCAG |
NGG |
2 |
0.2041 |
Tier II |
| 8 |
NC_000008.11 |
8 |
22201563 |
- |
BMP1 |
NNNGAGGATGGGGGTCCGAG |
NGG |
2 |
0.2 |
Tier II |
| 9 |
NC_000011.10 |
11 |
47414668 |
+ |
SLC39A13 |
NNNCAGGATGGGGGTCCCAG |
NGG |
2 |
0.1905 |
Tier II |
| 10 |
NC_000017.11 |
17 |
17119093 |
+ |
MPRIP |
NNNCAGGATGTGGGTTCCAG |
NGG |
2 |
0.1465 |
Tier II |
| 11 |
NC_000010.11 |
10 |
95400360 |
- |
SORBS1 |
NNNCAGGATGTGGTTCAGAG |
NGG |
2 |
0.1244 |
Tier II |
| 12 |
NC_000012.12 |
12 |
130829622 |
- |
STX2 |
NNNCAGGCTGTGGGCCCGAG |
NGG |
2 |
0.1169 |
Tier II |
| 13 |
NC_000012.12 |
12 |
4531815 |
- |
FERRY3 |
NNNCAGGATGTGGGGCAGAG |
NGG |
2 |
0.0233 |
Tier II |
| 14 |
NC_000007.14 |
7 |
158569506 |
+ |
PTPRN2 |
NNNCAGGCTGTGGGGCCGAG |
NGG |
2 |
0.0214 |
Tier II |
| 15 |
NC_000011.10 |
11 |
69090099 |
+ |
TPCN2 |
NNNCAGGATGTGGGTGCGAG |
NCG |
2 |
0.0165 |
Tier II |
| 16 |
NC_000013.11 |
13 |
100804744 |
- |
NALCN-AS1 |
NNNCAGGCTGTGGGTCCCAG |
NGG |
2 |
0.2041 |
Tier III |
| 17 |
NC_000015.10 |
15 |
39085333 |
+ |
LOC105370777 |
NNNCAGGATGTGGGTCAGAG |
NAG |
2 |
0.121 |
Tier III |
| 18 |
NC_000002.12 |
2 |
8133131 |
+ |
LINC00299 |
NNNCAGGATGTGGGGCAGAG |
NGG |
2 |
0.0233 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
121282351 |
- |
Farp1 |
NNNCAAGATGTGGGCCCGAG |
NGG |
2 |
0.2727 |
Tier I |
| 2 |
NC_000073.6 |
7 |
143495418 |
+ |
Slc22a18 |
NNNCAGGATGTAGGTCCAAG |
NGG |
2 |
0.6462 |
Tier II |
| 3 |
NC_000068.7 |
2 |
165695265 |
+ |
Eya2 |
NNNCAGGATGTGGGTACCAG |
NGG |
2 |
0.4762 |
Tier II |
| 4 |
NC_000077.6 |
11 |
6003043 |
- |
Camk2b |
NNNCAGGATGTGGGTCCCAG |
NGG |
1 |
0.4762 |
Tier II |
| 5 |
NC_000080.6 |
14 |
25631385 |
- |
Zmiz1 |
NNNCAGGAAGTGGGTCTGAG |
NGG |
2 |
0.4333 |
Tier II |
| 6 |
NC_000078.6 |
12 |
37388661 |
- |
Agmo |
NNNCAGGATGTGGGTCCGAC |
NGG |
1 |
0.4286 |
Tier II |
| 7 |
NC_000070.6 |
4 |
114427448 |
+ |
Trabd2b |
NNNCAGGATGTGGATCAGAG |
NGG |
2 |
0.35 |
Tier II |
| 8 |
NC_000079.6 |
13 |
102944839 |
- |
Mast4 |
NNNCAGGCTGTGGGTCCTAG |
NGG |
2 |
0.2857 |
Tier II |
| 9 |
NC_000084.6 |
18 |
75924630 |
+ |
Zbtb7c |
NNNCAGGATGTGGCTCTGAG |
NGG |
2 |
0.2 |
Tier II |
| 10 |
NC_000078.6 |
12 |
81748660 |
+ |
Map3k9 |
NNNCAGGATGTGGGTCCTAG |
NGC |
2 |
0.0148 |
Tier II |
| 11 |
NC_000073.6 |
7 |
36079189 |
+ |
Gm38991 |
NNNAATGATGTGGGTCCGAG |
NGG |
2 |
0.5614 |
Tier III |
| 12 |
NC_000079.6 |
13 |
102944839 |
- |
Gm30106 |
NNNCAGGCTGTGGGTCCTAG |
NGG |
2 |
0.2857 |
Tier III |
| 13 |
NC_000086.7 |
X |
3404454 |
+ |
Gm14352 |
NNNCAGGATGTGGGTCCCAG |
NGA |
2 |
0.0331 |
Tier III |
Other clones with same target sequence:
(none)
