Construct: sgRNA BRDN0001162228
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTTCTTCCAATGTAAACGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DCLK2 (166614)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75700
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
150220741 |
+ |
DCLK2 |
NNNTCTTCCAATGTAAACGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
105688879 |
- |
GRIA4 |
NNNTCTTCCAATATAAACAG |
NGG |
2 |
0.6593 |
Tier II |
| 3 |
NC_000016.10 |
16 |
72908312 |
+ |
ZFHX3 |
NNNTCTTCCATTGTAAAAGG |
NGG |
2 |
0.1657 |
Tier II |
| 4 |
NC_000006.12 |
6 |
8101303 |
+ |
EEF1E1 |
NNNTCTTCCAATTTAAACGG |
NTG |
2 |
0.0117 |
Tier II |
| 5 |
NC_000019.10 |
19 |
1006800 |
+ |
GRIN3B |
NNNTCTTCCAATGTAAACGC |
NGC |
2 |
0.0095 |
Tier II |
| 6 |
NC_000006.12 |
6 |
8101303 |
+ |
EEF1E1-BLOC1S5 |
NNNTCTTCCAATTTAAACGG |
NTG |
2 |
0.0117 |
Tier III |
| 7 |
NC_000009.12 |
9 |
134214525 |
- |
LOC107987137 |
NNNTCTTCCAATGGAAACCG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
86816396 |
- |
Dclk2 |
NNNTCTTCCAACGTAAACGG |
NGG |
1 |
0.9474 |
Tier I |
| 2 |
NC_000086.7 |
X |
93334276 |
- |
Pola1 |
NNNTCTTCAAATATAAACGG |
NGG |
2 |
0.7912 |
Tier II |
| 3 |
NC_000068.7 |
2 |
10099441 |
- |
Itih2 |
NNNTCTTCCAATGTAAACAT |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000077.6 |
11 |
104581558 |
- |
Myl4 |
NNNTCTTACAATGTAAACGC |
NGG |
2 |
0.2786 |
Tier II |
| 5 |
NC_000068.7 |
2 |
170371846 |
- |
Bcas1 |
NNNTCTTACACTGTAAACGG |
NGG |
2 |
0.26 |
Tier II |
| 6 |
NC_000074.6 |
8 |
11367713 |
+ |
Col4a2 |
NNNTCTTCCAATTTAAACAG |
NGG |
2 |
0.2143 |
Tier II |
| 7 |
NC_000068.7 |
2 |
170371846 |
- |
Bcas1os2 |
NNNTCTTACACTGTAAACGG |
NGG |
2 |
0.26 |
Tier III |
| 8 |
NC_000071.6 |
5 |
128706862 |
- |
Gm33995 |
NNNTCTTCCATTGTAAATGG |
NGG |
2 |
0.1978 |
Tier III |
Other clones with same target sequence:
(none)
