Construct: sgRNA BRDN0001162229
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGGATGCCTTCTACAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K6 (9064)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76015
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
27366264 |
- |
MAP3K6 |
NNNGGATGCCTTCTACAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
20807435 |
- |
EIF4G3 |
NNNGGATGCCTTCTACAAAT |
NGG |
2 |
0.3 |
Tier I |
| 3 |
NC_000005.10 |
5 |
159263655 |
- |
UBLCP1 |
NNNGGATGCCTTCAACAAGG |
NGG |
2 |
0.0774 |
Tier II |
| 4 |
NC_000012.12 |
12 |
109930927 |
- |
GIT2 |
NNNGGATGCCTTCTAGAAGG |
NGG |
2 |
0.0192 |
Tier II |
| 5 |
NC_000009.12 |
9 |
94825369 |
- |
AOPEP |
NNNAGATGCCTTCGACAACG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000002.12 |
2 |
162159511 |
- |
LOC101929532 |
NNNTGATTCCTTCTACAACG |
NGG |
2 |
0.2273 |
Tier III |
| 7 |
NC_000014.9 |
14 |
101470028 |
+ |
LINC03117 |
NNNGGATGCCTGCTACACCG |
NGG |
2 |
0.0952 |
Tier III |
| 8 |
NC_000017.11 |
17 |
5927587 |
- |
LOC339166 |
NNNGGAGGCCTTCTACAACG |
NTG |
2 |
0.0229 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
133241426 |
+ |
Map3k6 |
NNNGGACTCCTTCTACAACG |
NGG |
2 |
0.4297 |
Tier I |
| 2 |
NC_000070.6 |
4 |
138206039 |
+ |
Eif4g3 |
NNNGGATGCCTTCTACAAAT |
NGG |
2 |
0.3 |
Tier I |
| 3 |
NC_000068.7 |
2 |
126152995 |
+ |
Dtwd1 |
NNNGGATGCCTTCTACAGAG |
NGG |
2 |
0.1714 |
Tier II |
| 4 |
NC_000075.6 |
9 |
47755651 |
+ |
Cadm1 |
NNNGAATGCCTTCTACCACG |
NGG |
2 |
0.1529 |
Tier II |
| 5 |
NC_000072.6 |
6 |
136044364 |
- |
Grin2b |
NNNGGATGCGTTCCACAACG |
NGG |
2 |
0.1111 |
Tier II |
Other clones with same target sequence:
(none)
