Construct: sgRNA BRDN0001162232
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAGATAGTGCGGACACCCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DCLK2 (166614)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75699
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
150079382 |
- |
DCLK2 |
NNNATAGTGCGGACACCCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
120197463 |
+ |
RPLP0 |
NNNACATTGCGGACACCCTG |
NGG |
2 |
0.5714 |
Tier I |
| 3 |
NC_000007.14 |
7 |
143368132 |
- |
FAM131B |
NNNATGGTGAGGACACCCTG |
NGG |
2 |
0.619 |
Tier II |
| 4 |
NC_000011.10 |
11 |
282021 |
- |
NLRP6 |
NNNATGGTGAGGACACCCTG |
NGG |
2 |
0.619 |
Tier II |
| 5 |
NC_000021.9 |
21 |
36217677 |
- |
DOP1B |
NNNAAAGTGCAGACACCCTG |
NGG |
2 |
0.5 |
Tier II |
| 6 |
NC_000007.14 |
7 |
157955749 |
+ |
PTPRN2 |
NNNCTAGGGCGGACACCCTG |
NGG |
2 |
0.2588 |
Tier II |
| 7 |
NC_000007.14 |
7 |
22229050 |
+ |
RAPGEF5 |
NNNATAGTGGGGACACCATG |
NGG |
2 |
0.2094 |
Tier II |
| 8 |
NC_000010.11 |
10 |
3726482 |
- |
LOC105376360 |
NNNATAGTGAGGAAACCCTG |
NGG |
2 |
0.3033 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
142490473 |
- |
Ldhb |
NNNAGAGTGCGGACACCCTG |
NGG |
1 |
0.64 |
Tier I |
| 2 |
NC_000069.6 |
3 |
86919817 |
+ |
Dclk2 |
NNNATGGTGCGGACGCCCTG |
NGG |
2 |
0.4643 |
Tier I |
| 3 |
NC_000081.6 |
15 |
58943733 |
+ |
Mtss1 |
NNNATATTGCTGACACCCTG |
NGG |
2 |
0.2198 |
Tier I |
| 4 |
NC_000083.6 |
17 |
29367421 |
+ |
Fgd2 |
NNNAGAGTGAGGACACCCTG |
NGG |
2 |
0.5547 |
Tier II |
| 5 |
NC_000071.6 |
5 |
131029988 |
- |
Galnt17 |
NNNATAGTGTGGCCACCCTG |
NGG |
2 |
0.1981 |
Tier II |
| 6 |
NC_000079.6 |
13 |
23869135 |
+ |
Slc17a1 |
NNNATAGTGCGGACTTCCTG |
NGG |
2 |
0.0615 |
Tier II |
| 7 |
NC_000085.6 |
19 |
21938349 |
+ |
Ldhb-ps |
NNNAGAGTGCGGACACCCTG |
NGG |
1 |
0.64 |
Tier III |
| 8 |
NC_000070.6 |
4 |
114646472 |
+ |
Gm33655 |
NNNATATTGCAGACACCCTG |
NGG |
2 |
0.5714 |
Tier III |
Other clones with same target sequence:
(none)
