Construct: sgRNA BRDN0001162233

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TACTCAAAAAGCATGCACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ROR1 (4919)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76044

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 64177686 - ROR1 NNNTCAAAAAGCATGCACAC NGG 0 1.0 Tier I
2 NC_000002.12 2 74170923 + MOB1A NNNTCAAGAAGCATACACAC NGG 2 0.6902 Tier II
3 NC_000002.12 2 57942599 - VRK2 NNNTTAAAAAGCATACACAC NGG 2 0.5989 Tier II
4 NC_000003.12 3 196490698 + RNF168 NNNACAAAAAACATGCACAC NGG 2 0.4762 Tier II
5 NC_000021.9 21 40399486 - DSCAM NNNTCAGATAGCATGCACAC NGG 2 0.4235 Tier II
6 NC_000008.11 8 39658271 - ADAM18 NNNTGAAATAGCATGCACAC NGG 2 0.36 Tier II
7 NC_000001.11 1 217526007 - GPATCH2 NNNTCAAAAAGTATGCACAA NGG 2 0.2692 Tier II
8 NC_000003.12 3 152351821 + MBNL1 NNNACAAAAAGCATGCAAAC NGG 2 0.2564 Tier II
9 NC_000002.12 2 63191506 - WDPCP NNNTCAAAAAGCCTGAACAC NGG 2 0.2105 Tier II
10 NC_000008.11 8 68004500 - PREX2 NNNTCAATAAGGATGCACAC NGG 2 0.1905 Tier II
11 NC_000010.11 10 6107138 + RBM17 NNNTCAAAATGCATGGACAC NGG 2 0.1357 Tier II
12 NC_000001.11 1 204839085 - NFASC NNNTCAAAAAGCATGCATCC NGG 2 0.133 Tier II
13 NC_000018.10 18 44786273 - SETBP1 NNNTCAATAAGCATGTACAC NGG 2 0.1319 Tier II
14 NC_000019.10 19 48042982 + CABP5 NNNTTAAAAAGCATGCCCAC NGG 2 0.1123 Tier II
15 NC_000001.11 1 165639310 + MGST3 NNNGCAAAAAGCATGGACAC NGG 2 0.0962 Tier II
16 NC_000002.12 2 74267263 - SLC4A5 NNNTCAAAAGGCATGCACAC NGA 2 0.0386 Tier II
17 NC_000003.12 3 183408127 - MCF2L2 NNNTCAAAAATCATGCACAC NGA 2 0.0267 Tier II
18 NC_000005.10 5 5451865 + ICE1 NNNTCAAATAGCATGCACAC NTG 2 0.0234 Tier II
19 NC_000013.11 13 28005688 + FLT3 NNNTCAAAAAGGATGCACAC NTG 2 0.0173 Tier II
20 NC_000010.11 10 78665361 - LOC105378379 NNNTCAAAAGGCATGCAAAC NGG 2 0.2991 Tier III
21 NC_000023.11 X 131025551 - LINC01201 NNNTCACAAAGCAAGCACAC NGG 2 0.2708 Tier III
22 NC_000011.10 11 125683336 - LOC105369551 NNNTCAACAATCATGCACAC NGG 2 0.1648 Tier III
23 NC_000008.11 8 133163599 - LOC105375769 NNNTCAAAAAACATGCAGAC NGG 2 0.1333 Tier III
24 NC_000002.12 2 66015352 - LINC02934 NNNCCAAAAAGCATGCACAC NGA 2 0.0449 Tier III
25 NC_000006.12 6 139825709 - FILNC1 NNNTCATAAAGCATGCACAC NTG 2 0.017 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 100441076 - Ror1 NNNTCAAAGAGCATACACAC NGG 2 0.6275 Tier I
2 NC_000080.6 14 4863939 - Gm3264 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier I
3 NC_000080.6 14 7650271 + Gm38418 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier I
4 NC_000073.6 7 29507430 + Sipa1l3 NNNTCAAAAAGCATGCACAC NGG 0 1.0 Tier II
5 NC_000072.6 6 61228697 - Ccser1 NNNTCAAGAAGCATACACAC NGG 2 0.6902 Tier II
6 NC_000075.6 9 27010728 - Vps26b NNNTCAGAAAGCATACACAC NGG 2 0.6644 Tier II
7 NC_000078.6 12 4892080 + Ubxn2a NNNTCAAAAAGCAAACACAC NGG 2 0.5826 Tier II
8 NC_000086.7 X 161085511 - Scml2 NNNCCAAAATGCATGCACAC NGG 2 0.5709 Tier II
9 NC_000086.7 X 161119637 - Scml2 NNNCCAAAATGCATGCACAC NGG 2 0.5709 Tier II
10 NC_000086.7 X 161152391 - Scml2 NNNCCAAAATGCATGCACAC NGG 2 0.5709 Tier II
11 NC_000067.6 1 52815513 - Inpp1 NNNTCCAAATGCATGCACAC NGG 2 0.4011 Tier II
12 NC_000083.6 17 34620169 + Ppt2 NNNTTAAAAAGCAAGCACAC NGG 2 0.3939 Tier II
13 NC_000077.6 11 50694099 - Adamts2 NNNTTAAAAAGCATGCAAAC NGG 2 0.3427 Tier II
14 NC_000080.6 14 54408055 - Slc7a7 NNNTCAAAAAGCAAGCAAAC NGG 2 0.3333 Tier II
15 NC_000068.7 2 139748916 - Ism1 NNNTCAGAAAGGATGCACAC NGG 2 0.3137 Tier II
16 NC_000086.7 X 36003721 - Dock11 NNNTCTATAAGCATGCACAC NGG 2 0.3061 Tier II
17 NC_000082.6 16 38543826 + Poglut1 NNNAGAAAAAGCATGCACAC NGG 2 0.2857 Tier II
18 NC_000074.6 8 39022172 - Tusc3 NNNTCCAAAAGCAAGCACAC NGG 2 0.2814 Tier II
19 NC_000079.6 13 108922967 + Pde4d NNNTCAGAAATCATGCACAC NGG 2 0.2715 Tier II
20 NC_000082.6 16 34063241 + Kalrn NNNGCAAAAACCATGCACAC NGG 2 0.2679 Tier II
21 NC_000080.6 14 23881112 + Kcnma1 NNNTCAATAAGTATGCACAC NGG 2 0.2308 Tier II
22 NC_000070.6 4 86838125 - Dennd4c NNNCCAAAACGCATGCACAC NGG 2 0.2157 Tier II
23 NC_000071.6 5 93141040 - Septin11 NNNTCAAAAGGCTTGCACAC NGG 2 0.1667 Tier II
24 NC_000084.6 18 77344295 - Loxhd1 NNNCCAAAAAGCATGCCCAC NGG 2 0.1142 Tier II
25 NC_000067.6 1 188583118 - Ush2a NNNACAAAAAGCATGCACCC NGG 2 0.0985 Tier II
26 NC_000077.6 11 68515281 + Pik3r6 NNNTCAAAAAGAATGCACAG NGG 2 0.042 Tier II
27 NC_000067.6 1 88229251 + Mroh2a NNNTCAAAAAGCATTCACAC NAG 2 0.037 Tier II
28 NC_000073.6 7 16580984 + Arhgap35 NNNTCAAAATGCATGCACAC NTG 2 0.0344 Tier II
29 NC_000074.6 8 9214846 - Fam155a NNNTAAAAAAGCATGCACAC NTG 2 0.0223 Tier II
30 NC_000075.6 9 104928478 - Cpne4 NNNTCAAAAAGCATGGTCAC NGG 2 0.0205 Tier II
31 NC_000071.6 5 7023932 - Zfp804b NNNTCAAAAACCATGCACAC NTG 2 0.0167 Tier II
32 NC_000078.6 12 79402907 - Rad51b NNNTCAACAAGCATGCACAC NTG 2 0.0167 Tier II
33 NC_000078.6 12 117700948 - Rapgef5 NNNTCAAACAGCATGCACAC NGT 2 0.0092 Tier II
34 NC_000075.6 9 74884508 + Onecut1 NNNTCAAAAAGCATGCACAC NCA 2 0.0 Tier II
35 NC_000080.6 14 3218080 - D830030K20Rik NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
36 NC_000080.6 14 3218080 - Gm38690 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
37 NC_000080.6 14 3957270 - Gm3095 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
38 NC_000080.6 14 4027463 - Gm5796 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
39 NC_000080.6 14 4027463 - Gm32966 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
40 NC_000080.6 14 4118860 - Gm8108 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
41 NC_000080.6 14 4118860 - Gm32966 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
42 NC_000080.6 14 4191004 - Gm2974 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
43 NC_000080.6 14 4341671 - 2610042L04Rik NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
44 NC_000080.6 14 4507541 - Gm3173 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
45 NC_000080.6 14 4657899 - Gm3239 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
46 NC_000080.6 14 4863939 - Gm26650 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
47 NC_000080.6 14 5157409 - Gm3317 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
48 NC_000080.6 14 5379706 + Gm3500 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
49 NC_000080.6 14 5451950 + Gm3194 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
50 NC_000080.6 14 5510050 - Gm3488 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
51 NC_000080.6 14 5732242 + Gm3373 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
52 NC_000080.6 14 5804441 + Gm3383 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
53 NC_000080.6 14 5952198 + Gm3248 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
54 NC_000080.6 14 6273806 + Gm3411 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
55 NC_000080.6 14 6418736 + Lamtor3-ps NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
56 NC_000080.6 14 6596858 + Gm33933 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
57 NC_000080.6 14 7019081 + Gm10406 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
58 NC_000080.6 14 7097101 + Gm3696 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
59 NC_000080.6 14 7097101 + Gm31035 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
60 NC_000080.6 14 7480285 + Gm3752 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
61 NC_000080.6 14 7559036 + Gm3558 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
62 NC_000080.6 14 19576742 + Gm2237 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier II
63 NC_000067.6 1 192931923 + Syt14 NNNTCAACAAGCAGGCACAC NGG 2 0.0 Tier II
64 NC_000083.6 17 3263572 + Gm34035 NNNTCAAAATGCATGCACAA NGG 2 0.4412 Tier III
65 NC_000067.6 1 188583118 - Gm36456 NNNACAAAAAGCATGCACCC NGG 2 0.0985 Tier III
66 NC_000078.6 12 8147506 - Gm33037 NNNTGAAAAAGCATGGACAC NGG 2 0.0923 Tier III
67 NC_000078.6 12 109535901 - Gm34081 NNNTCAACAAGCATGCACAC NCG 2 0.0459 Tier III
68 NC_000080.6 14 3420997 - Gm10409 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier III
69 NC_000080.6 14 3660413 - Gm3020 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier III
70 NC_000080.6 14 3818491 - Gm3002 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier III
71 NC_000080.6 14 4423822 - Gm3164 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier III
72 NC_000080.6 14 4734554 - Gm3252 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier III
73 NC_000080.6 14 5079419 - Gm8281 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier III
74 NC_000080.6 14 5157409 - Gm26552 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier III
75 NC_000080.6 14 5379706 + Gm41087 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier III
76 NC_000080.6 14 5732242 + Gm36476 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier III
77 NC_000080.6 14 5952198 + Gm51421 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier III
78 NC_000080.6 14 6418736 + Gm26630 NNNTCAAAAAGCATGCACAC NCT 2 0.0 Tier III
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Other clones with same target sequence:

(none)