Construct: sgRNA BRDN0001162235
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAGCAAAGGACGAAACAAA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000001.11 | 1 | 197717208 | + | DENND1B | NNNGCAAAGGAAGAAACAAA | NGG | 1 | 0.7143 | Tier II |
2 | NC_000011.10 | 11 | 94306493 | - | IZUMO1R | NNNGCAGAGGAAGAAACAAA | NGG | 2 | 0.5042 | Tier II |
3 | NC_000016.10 | 16 | 13049913 | - | SHISA9 | NNNGCAAATGACAAAACAAA | NGG | 2 | 0.4923 | Tier II |
4 | NC_000010.11 | 10 | 132126107 | - | JAKMIP3 | NNNGCAAAGGAGAAAACAAA | NGG | 2 | 0.4103 | Tier II |
5 | NC_000014.9 | 14 | 79723557 | - | NRXN3 | NNNGCAAAGGAGAAAACAAA | NGG | 2 | 0.4103 | Tier II |
6 | NC_000006.12 | 6 | 44256170 | + | POLR1C | NNNGAAAAGGAAGAAACAAA | NGG | 2 | 0.4082 | Tier II |
7 | NC_000006.12 | 6 | 44256170 | + | SLC35B2 | NNNGAAAAGGAAGAAACAAA | NGG | 2 | 0.4082 | Tier II |
8 | NC_000012.12 | 12 | 86822293 | + | MGAT4C | NNNGAAAAGGAAGAAACAAA | NGG | 2 | 0.4082 | Tier II |
9 | NC_000020.11 | 20 | 16505872 | + | KIF16B | NNNGAAAAGGAAGAAACAAA | NGG | 2 | 0.4082 | Tier II |
10 | NC_000014.9 | 14 | 23243151 | - | RNF212B | NNNGCAAAGGAAGAAAAAAA | NGG | 2 | 0.3333 | Tier II |
11 | NC_000005.10 | 5 | 113324114 | + | MCC | NNNGCAAGGGAGGAAACAAA | NGG | 2 | 0.3259 | Tier II |
12 | NC_000011.10 | 11 | 49168423 | - | FOLH1 | NNNGCAAAGGACCAAACTAA | NGG | 2 | 0.2105 | Tier II |
13 | NC_000006.12 | 6 | 51968018 | - | PKHD1 | NNNGCAAAGGAGGAAAAAAA | NGG | 2 | 0.2074 | Tier II |
14 | NC_000008.11 | 8 | 42319049 | - | IKBKB | NNNGCAAAGGAAGACACAAA | NGG | 2 | 0.1948 | Tier II |
15 | NC_000011.10 | 11 | 120767362 | - | GRIK4 | NNNGCAAAGGACCAAACAGA | NGG | 2 | 0.1579 | Tier II |
16 | NC_000011.10 | 11 | 66249642 | + | KLC2 | NNNGCAAAGGAGGAAACCAA | NGG | 2 | 0.0847 | Tier II |
17 | NC_000011.10 | 11 | 66249642 | + | LOC124902694 | NNNGCAAAGGAGGAAACCAA | NGG | 2 | 0.0847 | Tier II |
18 | NC_000005.10 | 5 | 59056347 | + | PDE4D | NNNGCAAAGGACGAAAGAAG | NGG | 2 | 0.045 | Tier II |
19 | NC_000001.11 | 1 | 167517966 | + | CD247 | NNNGCAAAGGAAGAAAGAAA | NGG | 2 | 0.042 | Tier II |
20 | NC_000016.10 | 16 | 61960720 | - | CDH8 | NNNGCAAAGGAAGAAAGAAA | NGG | 2 | 0.042 | Tier II |
21 | NC_000014.9 | 14 | 70726728 | + | MAP3K9 | NNNCCAAAGGACGAAAGAAA | NGG | 2 | 0.0311 | Tier II |
22 | NC_000008.11 | 8 | 26014500 | - | EBF2 | NNNGCAAAGGAGGAAAGAAA | NGG | 2 | 0.0261 | Tier II |
23 | NC_000017.11 | 17 | 29733155 | + | SSH2 | NNNGCAAAGGAAGAACCAAA | NGG | 2 | 0.0 | Tier II |
24 | NC_000005.10 | 5 | 113324114 | + | LOC124901045 | NNNGCAAGGGAGGAAACAAA | NGG | 2 | 0.3259 | Tier III |
25 | NC_000001.11 | 1 | 63277311 | + | LINC00466 | NNNGCACAGGATGAAACAAA | NGG | 2 | 0.2356 | Tier III |
26 | NC_000011.10 | 11 | 89676947 | + | FOLH1B | NNNGCAAAGGACCAAACTAA | NGG | 2 | 0.2105 | Tier III |
27 | NC_000012.12 | 12 | 2752333 | - | ITFG2-AS1 | NNNGCAAAGGAGGAAATAAA | NGG | 2 | 0.2074 | Tier III |
28 | NC_000014.9 | 14 | 100661197 | + | LINC00523 | NNNGCAAAGGAGGAAACCAA | NGG | 2 | 0.0847 | Tier III |
29 | NC_000002.12 | 2 | 234848750 | - | LOC101927896 | NNNGCAAAGGATGAAAGAAA | NGG | 2 | 0.0317 | Tier III |
30 | NC_000012.12 | 12 | 72925661 | + | LOC105369838 | NNNGCAAAGGACGAAAGAAA | NTG | 2 | 0.0023 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000069.6 | 3 | 59328734 | - | Igsf10 | NNNGCAAAAGACCAAACAAA | NGG | 2 | 0.2707 | Tier I |
2 | NC_000075.6 | 9 | 65879147 | - | Trip4 | NNNGGAAAGGACGAAACAAA | NAG | 2 | 0.1556 | Tier I |
3 | NC_000071.6 | 5 | 108206728 | + | Ccdc18 | NNNGCAAAGGATGAAAGAAA | NGG | 2 | 0.0317 | Tier I |
4 | NC_000070.6 | 4 | 122846166 | - | Ppt1 | NNNGCAAAGAAAGAAACAAA | NGG | 2 | 0.6667 | Tier II |
5 | NC_000084.6 | 18 | 77992965 | + | Epg5 | NNNAGAAAGGACGAAACAAA | NGG | 2 | 0.54 | Tier II |
6 | NC_000082.6 | 16 | 41926615 | - | Lsamp | NNNGCAAAGAAGGAAACAAA | NGG | 2 | 0.4148 | Tier II |
7 | NC_000068.7 | 2 | 5210057 | + | Ccdc3 | NNNGCAAAGGAAGAAATAAA | NGG | 2 | 0.3333 | Tier II |
8 | NC_000067.6 | 1 | 104868923 | + | Cdh20 | NNNGCCAAGGAAGAAACAAA | NGG | 2 | 0.3247 | Tier II |
9 | NC_000086.7 | X | 138287043 | - | Il1rapl2 | NNNGCAATGGAAGAAACAAA | NGG | 2 | 0.3061 | Tier II |
10 | NC_000075.6 | 9 | 104012383 | + | Nphp3 | NNNGTCAAGGACGAAACAAA | NGG | 2 | 0.2893 | Tier II |
11 | NC_000068.7 | 2 | 58684683 | + | Upp2 | NNNGCAAAGGTAGAAACAAA | NGG | 2 | 0.2198 | Tier II |
12 | NC_000075.6 | 9 | 122928637 | - | Zfp105 | NNNGCAGAGGACTAAACAAA | NGG | 2 | 0.2118 | Tier II |
13 | NC_000077.6 | 11 | 89680467 | + | Ankfn1 | NNNTCAAAGGATGAAACAAA | NGG | 2 | 0.1958 | Tier II |
14 | NC_000071.6 | 5 | 92687845 | - | Shroom3 | NNNGCAAAGGAAGAAGCAAA | NGG | 2 | 0.1374 | Tier II |
15 | NC_000082.6 | 16 | 74201844 | + | Robo2 | NNNGCAAAGGAAGAAGCAAA | NGG | 2 | 0.1374 | Tier II |
16 | NC_000067.6 | 1 | 11114047 | - | Prex2 | NNNGCAAAGGAGGAAACACA | NGG | 2 | 0.092 | Tier II |
17 | NC_000082.6 | 16 | 96248873 | + | B3galt5 | NNNGCAAAGGACCCAACAAA | NGG | 2 | 0.0902 | Tier II |
18 | NC_000080.6 | 14 | 7930712 | - | Flnb | NNNGCAAAGGAGGAAGCAAA | NGG | 2 | 0.0855 | Tier II |
19 | NC_000074.6 | 8 | 117260924 | - | Cmip | NNNGCAAAGGAAGAAAGAAA | NGG | 2 | 0.042 | Tier II |
20 | NC_000077.6 | 11 | 114740366 | - | Dnaic2 | NNNGCAAAGGACGAGAGAAA | NGG | 2 | 0.0382 | Tier II |
21 | NC_000075.6 | 9 | 70380493 | - | Myo1e | NNNGCAAAGGAGGAAACAAA | NTG | 2 | 0.0173 | Tier II |
22 | NC_000070.6 | 4 | 94304394 | + | Gm12648 | NNNGCAAAGAAAGAAACAAA | NGG | 2 | 0.6667 | Tier III |
23 | NC_000068.7 | 2 | 132509650 | - | 1700026D11Rik | NNNGCAAAGGACGAAACAAA | NAG | 1 | 0.2593 | Tier III |
24 | NC_000069.6 | 3 | 149337167 | + | Gm5149 | NNNGCAAAGGATGAAACGAA | NGG | 2 | 0.2154 | Tier III |
25 | NC_000069.6 | 3 | 149337167 | + | Gm30382 | NNNGCAAAGGATGAAACGAA | NGG | 2 | 0.2154 | Tier III |
26 | NC_000073.6 | 7 | 114324273 | - | 4933406I18Rik | NNNGCAAAGGAAGAAAGAAA | NGG | 2 | 0.042 | Tier III |