Construct: sgRNA BRDN0001162235

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCAGCAAAGGACGAAACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80261

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 197717208 + DENND1B NNNGCAAAGGAAGAAACAAA NGG 1 0.7143 Tier II
2 NC_000011.10 11 94306493 - IZUMO1R NNNGCAGAGGAAGAAACAAA NGG 2 0.5042 Tier II
3 NC_000016.10 16 13049913 - SHISA9 NNNGCAAATGACAAAACAAA NGG 2 0.4923 Tier II
4 NC_000010.11 10 132126107 - JAKMIP3 NNNGCAAAGGAGAAAACAAA NGG 2 0.4103 Tier II
5 NC_000014.9 14 79723557 - NRXN3 NNNGCAAAGGAGAAAACAAA NGG 2 0.4103 Tier II
6 NC_000006.12 6 44256170 + SLC35B2 NNNGAAAAGGAAGAAACAAA NGG 2 0.4082 Tier II
7 NC_000012.12 12 86822293 + MGAT4C NNNGAAAAGGAAGAAACAAA NGG 2 0.4082 Tier II
8 NC_000020.11 20 16505872 + KIF16B NNNGAAAAGGAAGAAACAAA NGG 2 0.4082 Tier II
9 NC_000014.9 14 23243151 - RNF212B NNNGCAAAGGAAGAAAAAAA NGG 2 0.3333 Tier II
10 NC_000005.10 5 113324114 + MCC NNNGCAAGGGAGGAAACAAA NGG 2 0.3259 Tier II
11 NC_000011.10 11 49168423 - FOLH1 NNNGCAAAGGACCAAACTAA NGG 2 0.2105 Tier II
12 NC_000006.12 6 51968018 - PKHD1 NNNGCAAAGGAGGAAAAAAA NGG 2 0.2074 Tier II
13 NC_000008.11 8 42319049 - IKBKB NNNGCAAAGGAAGACACAAA NGG 2 0.1948 Tier II
14 NC_000011.10 11 120767362 - GRIK4 NNNGCAAAGGACCAAACAGA NGG 2 0.1579 Tier II
15 NC_000011.10 11 66249642 + KLC2 NNNGCAAAGGAGGAAACCAA NGG 2 0.0847 Tier II
16 NC_000011.10 11 66249642 + LOC124902694 NNNGCAAAGGAGGAAACCAA NGG 2 0.0847 Tier II
17 NC_000005.10 5 59056347 + PDE4D NNNGCAAAGGACGAAAGAAG NGG 2 0.045 Tier II
18 NC_000001.11 1 167517966 + CD247 NNNGCAAAGGAAGAAAGAAA NGG 2 0.042 Tier II
19 NC_000016.10 16 61960720 - CDH8 NNNGCAAAGGAAGAAAGAAA NGG 2 0.042 Tier II
20 NC_000014.9 14 70726728 + MAP3K9 NNNCCAAAGGACGAAAGAAA NGG 2 0.0311 Tier II
21 NC_000008.11 8 26014500 - EBF2 NNNGCAAAGGAGGAAAGAAA NGG 2 0.0261 Tier II
22 NC_000017.11 17 29733155 + SSH2 NNNGCAAAGGAAGAACCAAA NGG 2 0.0 Tier II
23 NC_000005.10 5 113324114 + LOC124901045 NNNGCAAGGGAGGAAACAAA NGG 2 0.3259 Tier III
24 NC_000001.11 1 63277311 + LINC00466 NNNGCACAGGATGAAACAAA NGG 2 0.2356 Tier III
25 NC_000011.10 11 89676947 + FOLH1B NNNGCAAAGGACCAAACTAA NGG 2 0.2105 Tier III
26 NC_000012.12 12 2752333 - ITFG2-AS1 NNNGCAAAGGAGGAAATAAA NGG 2 0.2074 Tier III
27 NC_000014.9 14 100661197 + LINC00523 NNNGCAAAGGAGGAAACCAA NGG 2 0.0847 Tier III
28 NC_000002.12 2 234848750 - LOC101927896 NNNGCAAAGGATGAAAGAAA NGG 2 0.0317 Tier III
29 NC_000012.12 12 72925661 + LOC105369838 NNNGCAAAGGACGAAAGAAA NTG 2 0.0023 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 59328734 - Igsf10 NNNGCAAAAGACCAAACAAA NGG 2 0.2707 Tier I
2 NC_000075.6 9 65879147 - Trip4 NNNGGAAAGGACGAAACAAA NAG 2 0.1556 Tier I
3 NC_000071.6 5 108206728 + Ccdc18 NNNGCAAAGGATGAAAGAAA NGG 2 0.0317 Tier I
4 NC_000070.6 4 122846166 - Ppt1 NNNGCAAAGAAAGAAACAAA NGG 2 0.6667 Tier II
5 NC_000084.6 18 77992965 + Epg5 NNNAGAAAGGACGAAACAAA NGG 2 0.54 Tier II
6 NC_000082.6 16 41926615 - Lsamp NNNGCAAAGAAGGAAACAAA NGG 2 0.4148 Tier II
7 NC_000068.7 2 5210057 + Ccdc3 NNNGCAAAGGAAGAAATAAA NGG 2 0.3333 Tier II
8 NC_000067.6 1 104868923 + Cdh20 NNNGCCAAGGAAGAAACAAA NGG 2 0.3247 Tier II
9 NC_000086.7 X 138287043 - Il1rapl2 NNNGCAATGGAAGAAACAAA NGG 2 0.3061 Tier II
10 NC_000075.6 9 104012383 + Nphp3 NNNGTCAAGGACGAAACAAA NGG 2 0.2893 Tier II
11 NC_000068.7 2 58684683 + Upp2 NNNGCAAAGGTAGAAACAAA NGG 2 0.2198 Tier II
12 NC_000075.6 9 122928637 - Zfp105 NNNGCAGAGGACTAAACAAA NGG 2 0.2118 Tier II
13 NC_000077.6 11 89680467 + Ankfn1 NNNTCAAAGGATGAAACAAA NGG 2 0.1958 Tier II
14 NC_000071.6 5 92687845 - Shroom3 NNNGCAAAGGAAGAAGCAAA NGG 2 0.1374 Tier II
15 NC_000082.6 16 74201844 + Robo2 NNNGCAAAGGAAGAAGCAAA NGG 2 0.1374 Tier II
16 NC_000067.6 1 11114047 - Prex2 NNNGCAAAGGAGGAAACACA NGG 2 0.092 Tier II
17 NC_000082.6 16 96248873 + B3galt5 NNNGCAAAGGACCCAACAAA NGG 2 0.0902 Tier II
18 NC_000080.6 14 7930712 - Flnb NNNGCAAAGGAGGAAGCAAA NGG 2 0.0855 Tier II
19 NC_000074.6 8 117260924 - Cmip NNNGCAAAGGAAGAAAGAAA NGG 2 0.042 Tier II
20 NC_000077.6 11 114740366 - Dnaic2 NNNGCAAAGGACGAGAGAAA NGG 2 0.0382 Tier II
21 NC_000075.6 9 70380493 - Myo1e NNNGCAAAGGAGGAAACAAA NTG 2 0.0173 Tier II
22 NC_000070.6 4 94304394 + Gm12648 NNNGCAAAGAAAGAAACAAA NGG 2 0.6667 Tier III
23 NC_000068.7 2 132509650 - 1700026D11Rik NNNGCAAAGGACGAAACAAA NAG 1 0.2593 Tier III
24 NC_000069.6 3 149337167 + Gm5149 NNNGCAAAGGATGAAACGAA NGG 2 0.2154 Tier III
25 NC_000069.6 3 149337167 + Gm30382 NNNGCAAAGGATGAAACGAA NGG 2 0.2154 Tier III
26 NC_000073.6 7 114324273 - 4933406I18Rik NNNGCAAAGGAAGAAAGAAA NGG 2 0.042 Tier III
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Other clones with same target sequence:

(none)