Construct: sgRNA BRDN0001162236
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAGATCTATGCCATCCCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HK2 (3099)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76309
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
74867753 |
+ |
HK2 |
NNNGATCTATGCCATCCCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
204996850 |
+ |
NFASC |
NNNGCTCCATGCCATCCCTG |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000020.11 |
20 |
31257861 |
- |
DEFB115 |
NNNGATCTCAGCCATCCCTG |
NGG |
2 |
0.4898 |
Tier II |
| 4 |
NC_000004.12 |
4 |
30826540 |
+ |
PCDH7 |
NNNGACCTATGCCATCACTG |
NGG |
2 |
0.4242 |
Tier II |
| 5 |
NC_000018.10 |
18 |
58538820 |
+ |
ALPK2 |
NNNGATTTATGCCATCCCTC |
NGG |
2 |
0.3482 |
Tier II |
| 6 |
NC_000017.11 |
17 |
33110366 |
- |
ASIC2 |
NNNGATCTCTGGCATCCCTG |
NGG |
2 |
0.254 |
Tier II |
| 7 |
NC_000020.11 |
20 |
15291256 |
+ |
MACROD2 |
NNNCATCTATGCCATCACTG |
NGG |
2 |
0.2471 |
Tier II |
| 8 |
NC_000005.10 |
5 |
146969638 |
- |
PPP2R2B |
NNNGTTCTTTGCCATCCCTG |
NGG |
2 |
0.2182 |
Tier II |
| 9 |
NC_000004.12 |
4 |
76495367 |
- |
SHROOM3 |
NNNGTTCTAGGCCATCCCTG |
NGG |
2 |
0.1818 |
Tier II |
| 10 |
NC_000014.9 |
14 |
81328729 |
+ |
STON2 |
NNNGATCAATGCCCTCCCTG |
NGG |
2 |
0.1714 |
Tier II |
| 11 |
NC_000004.12 |
4 |
8851314 |
- |
HMX1 |
NNNGATCTAGGCCATCCCTG |
NGC |
2 |
0.0111 |
Tier II |
| 12 |
NC_000007.14 |
7 |
91485980 |
- |
LINC02932 |
NNNGATTAATGCCATCCCTG |
NGG |
2 |
0.65 |
Tier III |
| 13 |
NC_000022.11 |
22 |
47629717 |
+ |
LINC00898 |
NNNGATCTCAGCCATCCCTG |
NGG |
2 |
0.4898 |
Tier III |
| 14 |
NC_000013.11 |
13 |
22838159 |
- |
IPMKP1 |
NNNGATCGAGGCCATCCCTG |
NGG |
2 |
0.3667 |
Tier III |
| 15 |
NC_000023.11 |
X |
80574265 |
- |
HK2P1 |
NNNGATCTATGCCATCCCCG |
NGG |
1 |
0.2759 |
Tier III |
| 16 |
NC_000003.12 |
3 |
108719509 |
+ |
LOC105374033 |
NNNGATCTTTGCCATCCGTG |
NGG |
2 |
0.08 |
Tier III |
| 17 |
NC_000008.11 |
8 |
108383310 |
- |
LOC105375704 |
NNNGATCTATGCCATTCCTG |
NGC |
2 |
0.0068 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
100419913 |
- |
Ror1 |
NNNGATACATGCCATCCCTG |
NGG |
2 |
0.75 |
Tier II |
| 2 |
NC_000082.6 |
16 |
75573421 |
+ |
Lipi |
NNNGATCTATGCCAACCCTG |
NGG |
1 |
0.5789 |
Tier II |
| 3 |
NC_000067.6 |
1 |
191978717 |
+ |
Rd3 |
NNNGATCCATGCCATCTCTG |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000073.6 |
7 |
126289173 |
- |
Sbk1 |
NNNGATCAATGGCATCCCTG |
NGG |
2 |
0.3556 |
Tier II |
| 5 |
NC_000069.6 |
3 |
152787164 |
+ |
Pigk |
NNNGATCTATGCTGTCCCTG |
NGG |
2 |
0.3267 |
Tier II |
| 6 |
NC_000071.6 |
5 |
50050780 |
- |
Adgra3 |
NNNCATCTATCCCATCCCTG |
NGG |
2 |
0.2269 |
Tier II |
| 7 |
NC_000068.7 |
2 |
91136511 |
+ |
Mybpc3 |
NNNGATCTGTGCCATTCCTG |
NGG |
2 |
0.2051 |
Tier II |
| 8 |
NC_000075.6 |
9 |
97942499 |
+ |
Clstn2 |
NNNGATCTATGCCATCCCTG |
NGC |
1 |
0.0222 |
Tier II |
| 9 |
NC_000075.6 |
9 |
77176062 |
+ |
Mlip |
NNNGATCTATGCCTTCCCTG |
NGC |
2 |
0.0119 |
Tier II |
| 10 |
NC_000077.6 |
11 |
9570019 |
+ |
Abca13 |
NNNGATCTATTCCATCCCTG |
NGT |
2 |
0.0062 |
Tier II |
| 11 |
NC_000078.6 |
12 |
79924784 |
+ |
9430078K24Rik |
NNNGAGCTATACCATCCCTG |
NGG |
2 |
0.5714 |
Tier III |
| 12 |
NC_000078.6 |
12 |
79924784 |
+ |
Gm51985 |
NNNGAGCTATACCATCCCTG |
NGG |
2 |
0.5714 |
Tier III |
| 13 |
NC_000069.6 |
3 |
146347786 |
- |
Gm40167 |
NNNGATCTCTGCCATCCCTC |
NGG |
2 |
0.2449 |
Tier III |
| 14 |
NC_000071.6 |
5 |
50050780 |
- |
LOC115490186 |
NNNCATCTATCCCATCCCTG |
NGG |
2 |
0.2269 |
Tier III |
| 15 |
NC_000074.6 |
8 |
45175280 |
+ |
Gm30504 |
NNNCTTCTATGCCATCCCTG |
NGG |
2 |
0.1925 |
Tier III |
Other clones with same target sequence:
(none)
